RESUMO
Student-athletes at the Division I institutions face a slew of challenges and stressors that can have negative impacts in eliciting different emotional responses during the COVID-19 pandemic. We employed machine-learning-based natural language processing techniques to analyze the user-generated content posted on Twitter of Atlantic Coast Conference (ACC) student-athletes to study changes in their sentiment as it relates to the COVID-19 crisis, major societal events, and policy decisions. Our analysis found that positive sentiment slightly outweighed negative sentiment overall, but that there was a noticeable uptick in negative sentiment in May and June 2020 in conjunction with the Black Lives Matter protests. The most commonly expressed emotions by these athletes were joy, trust, anticipation, and fear, suggesting that they used social media as an outlet to share primarily optimistic sentiments, while still publicly expressing strong negative sentiments like fear and trepidation about the pandemic and other important contemporary events. Athletic administrators, ACC coaches, support staff, and other professionals can use findings like these to guide sound, evidence-based decision-making and to better track and promote the emotional wellness of student-athletes.
RESUMO
Ips pini bark beetles use myrcene hydroxylases to produce the aggregation pheromone component, ipsdienol, from myrcene. The enantiomeric ratio of pheromonal ipsdienol is an important prezygotic mating isolation mechanism of I. pini and differs among geographically distinct populations. We explored the substrate and product ranges of myrcene hydroxylases (CYP9T2 and CYP9T3) from reproductively-isolated western and eastern I. pini. The two cytochromes P450 share 94% amino acid identity. CYP9T2 mRNA levels were not induced in adults exposed to myrcene-saturated atmosphere. Functional assays of recombinant enzymes showed both hydroxylated myrcene, (+)- and (-)-α-pinene, 3-carene, and R-(+)-limonene, but not α-phellandrene, (-)-ß-pinene, γ-terpinene, or terpinolene, with evidence that CYP9T2 strongly preferred myrcene over other substrates. They differed in the enantiomeric ratios of ipsdienol produced from myrcene, and in the products resulting from different α-pinene enantiomers. These data provide new information regarding bark beetle pheromone evolution and factors affecting cytochrome P450 structure-function relationships.