RESUMO
Retinoic acid receptor α (RARA), a retinoic acid-dependent transcription factor, is expressed in both somatic and germ cells of the testis. Rara-null male mice with global Rara mutations displayed severely degenerated testis and infertility phenotypes. To elucidate the specific responsibility of germ cell RARA in spermatogenesis, Rara was deleted in germ cells, generating germ cell-specific Rara conditional knockout (cKO) mice. These Rara cKO animals exhibited phenotypes of quantitatively reduced epididymal sperm counts and disorganized germ cell layers in the seminiferous tubules, which worsened with aging. Abnormal tubules lacked lumen, contained vacuoles, and showed massive germ cell sloughing, all characteristics similar to those observed in Rara-null tubules. Spermatocyte chromosomal spreads revealed a novel role for germ cell RARA in modulating the integrity of synaptonemal complexes and meiotic progression. Furthermore, the initiation of spermatogenesis from spermatogonial stem cells was decreased in Rara cKO testes following busulfan treatment, supporting a role of germ cell RARA in spermatogonial proliferation. Collectively, the evidence in this study indicates that RARA produced in male germ cells has a broad spectrum of functions throughout spermatogenesis, which includes the maintenance of seminiferous epithelium organization, the integrity of the meiotic genome, and spermatogonial proliferation and differentiation. The results further suggest that germ cell RARA has dual functions: intrinsically in germ cells, balancing proliferation and differentiation of spermatogonia, and controlling genome integrity during meiosis; and extrinsically in the crosstalks with Sertoli cells, controlling the cell junctional physiology for coordinating proper spatial and temporal development of germ cells during spermatogenesis.
Assuntos
Meiose/genética , Receptor alfa de Ácido Retinoico/genética , Espermatogênese/genética , Espermatozoides/metabolismo , Animais , Diferenciação Celular/genética , Proliferação de Células/genética , Epididimo , Células Germinativas , Junções Intercelulares/metabolismo , Masculino , Camundongos , Camundongos Knockout , Receptor alfa de Ácido Retinoico/metabolismo , Epitélio Seminífero/metabolismo , Túbulos Seminíferos/metabolismo , Células de Sertoli/metabolismo , Transdução de Sinais , Contagem de Espermatozoides , Espermatócitos/crescimento & desenvolvimento , Espermatócitos/metabolismo , Espermatogônias/crescimento & desenvolvimento , Espermatogônias/metabolismo , TestículoRESUMO
A series of 5-fluorocytosine derivatives, 5'-deoxy-N-alkyloxycarbonyl-5-fluorocytosine-5'-carboxylic acid 6, were synthesized and evaluated for their antitumor activity.
