RESUMO
A marine bacterial strain, designated A71(T), was isolated from marine algae collected from the South Sea, Republic of Korea. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain A71(T) belonged to the family Flavobacteriaceae and was closely related to Aequorivita antarctica SW49(T) (96.5 % sequence similarity). Cells of strain A71(T) were Gram-negative, aerobic, oxidase-negative, catalase-positive, yellow/orange-pigmented and non-motile. The major fatty acids were iso-C(15 : 0) (20.6 %), iso-C(17 : 1)omega9c (13.3 %), anteiso-C(15 : 0) (13.1 %), iso-C(17 : 0) 3-OH (12.7 %) and summed feature 3 (comprising iso-C(15 : 0) 2-OH and/or C(16 : 1)omega7c; 6.6 %). The DNA G+C content was 36.9 mol%. Several phenotypic characteristics served to differentiate the isolate from recognized members of the genus Aequorivita. Data from this polyphasic study clearly demonstrated that strain A71(T) represents a novel species of the genus Aequorivita. The name Aequorivita capsosiphonis sp. nov. is proposed, with strain A71(T) (=KCTC 22183(T) =JCM 15070(T)) as the type strain. In addition, an emended description of the genus Aequorivita is presented.
Assuntos
Clorófitas/microbiologia , Flavobacteriaceae/classificação , Flavobacteriaceae/isolamento & purificação , Técnicas de Tipagem Bacteriana , Composição de Bases , Análise por Conglomerados , DNA Bacteriano/química , DNA Bacteriano/genética , DNA Ribossômico/química , DNA Ribossômico/genética , Ácidos Graxos/análise , Flavobacteriaceae/genética , Flavobacteriaceae/fisiologia , Coreia (Geográfico) , Dados de Sequência Molecular , Filogenia , RNA Ribossômico 16S/genética , Análise de Sequência de DNA , Homologia de Sequência do Ácido NucleicoRESUMO
A non-motile and rod shaped bacterium, designated strain B1(T), was isolated from forest soil at Mt. Baekwoon, Republic of Korea. Cells were Gram-negative, catalase-positive, and oxidase-negative. The major fatty acids were 9-octadecenoic acid (C(18:1) omega9c; 42%) and hexadecanoic acid (C(16:0); 25.9%) and summed feature 3 (comprising iso-C(15:0) 2-OH and/or C(16:1) omega7c; 10.0%). The DNA G+C content was 44.1 mol%. A phylogenetic tree based on 16S rRNA gene sequences showed that strain B1(T) formed a lineage within the genus Acinetobacter and was closely related to A. baylyi DSM 14961(T) (98.6% sequence similarity), followed by A. baumannii DSM 30007(T) (97.4%), A. calcoaceticus DSM 30006(T) (97.0%) and 3 genomic species (96.8 approximately 7.6%). Phenotypic characteristics, gyrB gene sequence analysis and DNA-DNA relatedness data distinguished strain B1(T) from type strains of A. baylyi, A. baumannii, and A. calcoaceticus. On the basis of the evidence presented in this study, strain B1(T) represents a novel species of the genus Acinetobacter, for which the name Acinetobacter soli sp. nov. is proposed. The type strain is B1(T) (= KCTC 22184(T)= JCM 15062(T)).
