Proteomic Analysis Reveals Salt-Tolerant Mechanism in Soybean Applied with Plant-Derived Smoke Solution.

Salt stress of soybean is a serious problem because it reduces plant growth and seed yield. To investigate the salt-tolerant mechanism of soybean, a plant-derived smoke (PDS) solution was used. Three-day-old soybeans were subjected to PDS solution under 100 mM NaCl for 2 days, resulting in PDS solution improving soybean root growth, even under salt stress. Under the same condition, proteins were analyzed using the proteomic technique. Differential abundance proteins were associated with transport/formaldehyde catabolic process/sucrose metabolism/glutathione metabolism/cell wall organization in the biological process and membrane/Golgi in the cellular component with or without PDS solution under salt stress. Immuno-blot analysis confirmed that osmotin, alcohol dehydrogenase, and sucrose synthase increased with salt stress and decreased with additional PDS solution; however, H+ATPase showed opposite effects. Cellulose synthase and xyloglucan endotransglucosylase/hydrolase increased with salt and decreased with additional PDS solution. Furthermore, glycoproteins decreased with salt stress and recovered with additional treatment. As mitochondrion-related events, the contents of ATP and gamma-aminobutyric acid increased with salt stress and recovered with additional treatment. These results suggest that PDS solution improves the soybean growth by alleviating salt stress. Additionally, the regulation of energy metabolism, protein glycosylation, and cell wall construction might be an important factor for the acquisition of salt tolerance in soybean.

Race categorization in noise.

People are typically faster to categorize the race of a face if it belongs to a race different from their own. This Other Race Categorization Advantage (ORCA) is thought to reflect an enhanced sensitivity to the visual race signals of other race faces, leading to faster response times. The current study investigated this sensitivity in a cross-cultural sample of Swiss and Japanese observers with a race categorization task using faces that had been parametrically degraded of visual structure, with normalized luminance and contrast. While Swiss observers exhibited an increasingly strong ORCA in both reaction time and accuracy as the face images were visually degraded up to 20% structural coherence, the Japanese observers manifested this pattern most distinctly when the faces were fully structurally-intact. Critically, for both observer groups, there was a clear accuracy effect at the 20% structural coherence level, indicating that the enhanced sensitivity to other race visual signals persists in significantly degraded stimuli. These results suggest that different cultural groups may rely on and extract distinct types of visual race signals during categorization, which may depend on the available visual information. Nevertheless, heavily degraded stimuli specifically favor the perception of other race faces, indicating that the visual system is tuned by experience and is sensitive to the detection of unfamiliar signals.

Computational chemical analysis of Eu(iii) and Am(iii) complexes with pnictogen-donor ligands using DFT calculations.

We demonstrated density functional calculations of Eu(iii) and Am(iii) complexes with pnictogen-donor (X) ligands, (CH3)2X-CH2-CH2-X(CH3)2 (X = N, P, As and Sb). We investigated the optimized structures of the complexes and the Gibbs energy differences in the complex formation reactions. The results indicated that the N- and P-donor ligands exhibit Am(iii) ion selectivity over Eu(iii) ions; especially, the P-donor ligand showed the highest selectivity. The tendency of the Am(iii)/Eu(iii) selectivity by the pnictogen-donor ligands was found to be comparable to that of the soft acid classification in the hard and soft acids and bases rule. Mulliken's spin population analysis indicated that the bonding properties between the metal ion and the pnictogen atoms correlated with the Am(iii)/Eu(iii) selectivity. In particular, the participation of f-orbital electrons of the metal ion in the covalency was indicated to play an important role in the selectivity.