High resolution MRI of the brain at 4.7 Tesla using fast spin echo imaging.

Over recent years, high field MR scanners (3 T and above) have become increasingly widespread due to potential advantages such as higher signal-to-noise ratio. However, few examples of high resolution images covering the whole brain in reasonable acquisition times have been published to date and none have used fast spin echo (FSE), a sequence commonly employed for the acquisition of T(2) weighted images at 1.5 T. This is mostly due to the increased technical challenges associated with uniform signal generation and the increasingly restrictive constraints of current safety guidelines at high field. We investigated 10 volunteers using an FSE sequence optimized to the 4.7 T environment. This sequence allows the acquisition of 17- and 34-slice data sets with an in-plane resolution of approximately 500 microm x 500 microm and a slice thickness of 2 mm, in 5 min 40 s and 11 min 20 s, respectively. The images appear T(2) weighted, although the contrast is due to the combined effects of chosen echo time, magnetization transfer, direct radio frequency saturation and diffusion as well as the T(1) and T(2) relaxation times of the tissue. The result is an excellent detailed visualization of anatomical structures, demonstrating the great potential of 4.7 T MRI for clinical applications. This paper shows that, with careful optimization of sequence parameters, FSE imaging can be used at high field to generate images with high spatial resolution and uniform contrast across the whole brain within the prescribed power deposition limits.

Stray field nuclear magnetic resonance of soil water: development of a new, large probe and preliminary results.

Development, characterization, and preliminary results of a recent technique capable of local measurements of pore-size distribution by a spatially resolved low resolution nuclear magnetic resonance (NMR) technique are described. Potential environmental uses include studying the change in pore-size distribution caused by surface compaction, which influences surface runoff, and obtaining information on the physical state of non-aqueous compounds in porous materials, which should aid the selection of appropriate soil remediation methods. Stray field (STRAFI) imaging is an NMR technique that allows distortion-free imaging of materials with short NMR relaxation times. The sample is placed in the strong axial fringe field gradient of a superconducting NMR magnet. We report on a new, unique, large 5-cm-diameter STRAFI probe, and its use for three preliminary test cases: water in ceramics of known pore size, paraffin wax and oil in sandstone rock, and water in soil at different matric potentials. The imaging is confined to one dimension with a spatial resolution of the order of 100 microm for protons. The optimum position for imaging occurs at 2.62 T and a gradient of 12.1 T/m. Water relaxation decay curves can be measured at any position in the 8-cm-long sample. These curves are decomposed into a series of terms each corresponding to a different pore size. Preliminary results show continuum fits to decay curves for a soil drained to three different matric potentials. Such information will be useful for interpreting water retention curves and will lead to understanding of the behavior of fluids in the vadose zone.

STRAFI-NMR studies of water transport in soil.

1-D STRAFI (STRAy FIeld) imaging is used to study water distribution in a sandy loam. The matric potential of the soil can be varied during acquisition of 1-D profiles. Results at a range of potentials are presented showing both the equilibrium distribution and the evolution of the profile following an abrupt change in matric potential. The air breakthrough point and variations in draining behaviour due to differences in soil compaction are identified.

Nuclear magnetic imaging of an osmotic water uptake and delivery process.

The water absorption of NH4F-doped silicone cylinders was studied by spin-echo Fourier nuclear magnetic resonance imaging, in conjunction with gravimetric (mass uptake) and ionic concentration (release of NH4F) measurements. The addition of NH4F greatly increases the water uptake as the inclusions are surrounded by droplets of water when wetted by water diffusing in the polymer. These droplets then expand due to the osmotic pressure difference between the internal droplet and external solution; this expansion is opposed by an elastic restraining force. The rate of ingress into the polymer will depend on the relative chemical potential of water associated with the polymer and the droplet, as well as the diffusion coefficient. Later stages of the absorption process are dominated by the droplets. The dilution of the droplets (from water diffusing into the droplets) slowly reduces the chemical potential driving the process. Hence, the later stages occur without any notable concentration gradient but a slight chemical potential gradient. If the droplet expansion exceeds that which can be restrained by the material, failure around the droplet occurs resulting in the release of the salt and the solution within the droplet.

Calibration of the stray field gradient by a heteronuclear method and by field profiling

Heteronuclear and field-profiling stray field (STRAFI) techniques are used to calibrate the STRAFI gradient. Both methods compare very favorably indeed with the conventional method of calibration which uses a standard with a known self-diffusion constant. The distinct advantages of the techniques presented here are that the constraints on both sample purity and sample temperature that are inherent to the conventional method are completely eliminated. The accuracy of the heteronuclear method typically matches that of the conventional method with a pure sample and temperature stability to within 0.4 degrees C. The field-profiling method is more accurate than the heteronuclear method in the form that it is presented here. Copyright 2000 Academic Press.

Fetal programming of hepatic lobular architecture in the rat demonstrated ex vivo with magnetic resonance imaging.

We demonstrate that MRI imaging at sub-millimetre resolution can distinguish between periportal and perivenous zones of the rat liver lobule. This made it possible to measure the hepatic lobular radius in ex-vivo perfused fixed livers using MRI. Comparisons of histomorphometric and MRI measurements of lobular radius were in good agreement, although MRI measurements were significantly smaller (P< 0.001). Male rats whose mothers were fed 40% of the protein of controls during gestation and lactation, had a significantly larger hepatic lobular radius than that of controls [449+/-11 microm vs. 373+/-9 microm (mean +/- SEM), respectively, p<0.001, n = 12; histomorphometry data]. The proton T(2) in periportal and perivenous zones was mapped both before and after antegrade or retrograde perfusion of 10 ml of digitonin (4 mg ml(-1)). Only the T(2) of the hypointense regions increased significantly following antegrade perfusion of digitonin and conversely only that of the intense regions following retrograde perfusion. Digitonin causes permeabilization of cells in specific hepatic zones, determined by the direction of perfusion. The intense and hypointense regions of the hepatic MR images thus arise from the perivenous and periportal zones of the hepatic lobule, respectively.

Magnetic resonance imaging (MRI) as a method to investigate movement of water during the extrusion of pastes.

PURPOSE: To assess the potential of Magnetic Resonance Imaging (MRI) as a method of detecting water movement during the extrusion of pastes. METHODS: Plug samples were made from mixtures of model materials and microcrystalline cellulose with two water contents at two different ram speeds to simulate ram extrusion. The extrusion process was stopped at different stages and analyzed for water distribution using MRI to assess the influence of water content and the speed of ram on water movement as the extrusion process progresses. RESULTS: Two types of water movement were detected: vertical and radial. When extruding at the faster ram speed, water moved predominantly in the vertical direction, whereas when extruding at a slower ram speed it moved predominantly in the radial direction. At the beginning of the extrusion process a greater water movement in the wetter formulations was observed. CONCLUSIONS: MRI appears to be a useful approach to non-invasive water mapping, and is expected to contribute towards a greater understanding of the role of water in the extrusion of pastes.

A new ytterbium chelate as contrast agent in chemical shift imaging and temperature sensitive probe for MR spectroscopy.

A paramagnetic Yb(III) complex that is the prototype of a novel class of probes for MRI and MRS has been developed. The complex displays highly shifted 1H resonances that are characterized by short relaxation times and, as such, may prove to be a valuable alternative in applications that currently require fluorine-containing probes. Selective excitation of the paramagnetically shifted resonances allows the spatial distribution of the complex to be mapped. This communication reports the images that were obtained by selectively exciting the most intense methyl group (-14.2 ppm at 27 degrees C) for complex concentrations ranging from 0.003-0.1 M. Spectroscopically, the complex may be used as a temperature probe since the proton chemical shifts exhibit a strong temperature dependence. In human serum the chemical shift difference of a selected pair of proton resonances was observed to follow a gradient of -0.42 +/- 0.01 ppm/degrees C. Furthermore, since the chemical shift of the methyl resonance displays a temperature coefficient of -0.04 +/- 0.01 ppm/degrees C, it should be possible to use the image phase for thermal mapping.

Magnetic susceptibility effects in imaging: distortion-free images of plant tissue in soil.

Magnetic susceptibility effects (MSE) in NMR spectra are well known, and indeed the NMR technique has frequently been applied to measure magnetic susceptibility. In the case of imaging, MSE can lead to image distortion when the sample is heterogeneous. We have performed experiments on a soil sample (iron content approximately 2%) containing plant tissue which gave a NMR signal that was spread over about 15 kHz in the 1H spectrum. We present some results from a 128 x 128 x 128 3D 1H image (voxel size = 150 x 150 x 150 micron3) generated by the stray field imaging (STRAFI) technique in which the use of a 5 kG cm-1 magnetic flux density gradient reduced the magnetic susceptibility distortion to less than 10 microns.