Contrast-Specific Spherical Lesion Phantoms and Ancillary Analysis Software for the Objective Evaluation of Transrectal Ultrasound System Contrast Detectability.

Brachytherapy is an efficacious treatment option because of its benefits for patient recovery, dose localization and conformity, but these favorable outcomes can be ensured only if the transrectal ultrasound (TRUS) system is optimized for the specific application of ultrasound-guided prostate brachytherapy. The ability to delineate the prostate from surrounding tissue during TRUS-guided prostate brachytherapy is vital for treatment planning, and consequently, so is the contrast resolution. This study describes the development of task-specific contrast-detail phantoms with clinically relevant contrast and spherical target sizes for contrast-detail performance evaluation of TRUS systems used in the brachytherapy procedure. The procedure for objective assessment of the contrast detectability of the TRUS systems is also described; a program was developed in MATLAB (R2017a, The MathWorks, Natick, MA, USA) to quantitatively analyze image quality in terms of the lesion signal-to-noise ratio (LSNR) and validated with representative control test images. The LSNR of the Hitachi EUB-7500A (2013, Hitachi, Ltd, Tokyo, Japan) TRUS system was measured on sagittal and transverse TRUS images of the contrast-detail phantoms described in this work. Results revealed the efficacy of the device as an image quality evaluation tool and the impact of the size, depth and relative contrast of the targets to the surrounding tissue on the contrast detectability of a TRUS system for both transducer arrays. The MATLAB program objectively measured the contrast detectability of the TRUS system and has the potential to determine optimized imaging parameters that could be designed as part of standardization of the imaging protocol used in TRUS-guided prostate brachytherapy for prostate cancer.

Use of a novel anthropomorphic prostate simulator in a prostate brachytherapy transrectal ultrasound imaging workshop for medical physicists.

PURPOSE: Ultrasound imaging training is not required as part of radiation oncology training programs nor does any objective competency measure exist to independently assess performance. Physical simulation training can provide a structured approach to this training but only if suitably challenging training simulators exist. This study describes the design and preliminary evaluation of a simulation-based transrectal ultrasound (TRUS) imaging training workshop developed for medical physicists involved in low-dose-rate (LDR) prostate brachytherapy (PBT). METHODS: The study incorporated novel high-fidelity anthropomorphic PBT TRUS training simulators and a TRUS imaging module with a blended-learning pedagogical approach, to address TRUS image optimisation and managing image quality. RESULTS: Results demonstrated a significant improvement in knowledge, with an average increase in multiple choice question score of 61% (P < 0.0002), and that there was a 46% (P < 0.0001) average increase in the participants perceived understanding of TRUS scanner operation, and an increase of 36% (P < 0.001) in participants readiness to optimise image quality and mitigate image artefacts. Focus group data explored participants' experiences, perceptions and challenges with TRUS LDR PBT. CONCLUSIONS: This study suggests a benefit in offering a simulation training workshop to medical physicists and the potential benefit to other healthcare professionals involved in prostate brachytherapy, by incorporating novel high-fidelity anthropomorphic PBT TRUS training simulators, in a simulated environment to practice ultrasound image optimisation for PBT image guidance. This approach to training would enable competency-based skill acquisition and continued proficiency or health professionals in the TRUS PBT procedure, outside of the surgical environment without direct exposure to patients.

Development and Preliminary Evaluation of an Anthropomorphic Trans-rectal Ultrasound Prostate Brachytherapy Training Phantom.

The quality of the trans-rectal ultrasound (TRUS) image, and thus seed placement during the prostate brachytherapy (PBT) procedure, relies on the user's technical and clinical competency. Simulation-based medical education can provide a structured approach for the acquisition of clinical competencies, but the efficacy of the training relies on the fidelity of the training simulators. In this work, the design, development and preliminary evaluation of an anthropomorphic training phantom for TRUS PBT is described. TRUS clinical patient data informed the design of 3-D printed moulds to fabricate prostate targets. Tissue-mimicking materials were included that had the sonographic characteristics of the prostate and overlying tissues, as well as the clinically relevant physical response, to provide haptic feedback to the user. Through an iterative design process, prototypes were constructed. These prototypes were quantitatively evaluated using a specification list and evaluated by an experienced clinical brachytherapy oncologist; their feedback was implemented, and the results of this evaluation are presented.

Not Reinventing the Wheel: Adapting Treatment Foster Care Oregon to Public Mental Health.

Intervention adaptations expand the availability of evidence-based treatments across clinical settings and demographically different populations. These adaptations utilize systematic strategies to preserve core components of an intervention. Intervention adaptations made from one public system to another (e.g., juvenile justice to mental health) reduce the need to invent a new intervention. In this column, the authors discuss the adaptation of Treatment Foster Care Oregon, an evidence-based program for treating youths with serious emotional and behavioral disturbance, to Michigan's public mental health system. Challenges encountered in this adaptation and solutions are presented.

Perception of medical radiation risk in Ireland: Results of a public survey.

The Basic Safety Standards (BSS) directive 2013/59/EURATOM places a new emphasis on the practitioner and/or referrer to inform the patient of the benefits and risks associated with radiation dose. To communicate effectively, a prior knowledge of what a person comprehends is beneficial. The aim of this study was to evaluate the Irish public's current level of understanding of ionising radiation and its associated risks. We designed a survey consisting of ten multiple choice questions and asked members of the public to respond. There were 326 responses to the public survey. All survey data was collected anonymously using snowball sampling. Overall, the data collected from this survey indicates that the respondents do not have a clear understanding of radiation risks. In particular there was a misunderstanding in which modalities use ionising radiation and there was a perceived limit in the number of X-rays a person can have in a year, implying that the public have not been informed of the principle of justification. Patients must be presented with the benefits of an exam involving ionising radiation together with a clear explanation of the risks.

An investigation of the detection capability of pulsed wave duplex Doppler of low grade stenosis using ultrasound contrast agent microbubbles - An in-vitro study.

OBJECTIVE: The objective of the study was to investigate whether clinically used ultrasonic contrast agents improved the accuracy of spectral Doppler ultrasound in the detection of low grade (<50%) renal artery stenosis. Low grade stenoses in the renal artery are notoriously difficult to reliably detect using Doppler ultrasound due to difficulties such as overlying fat and bowel gas. METHODS: A range of anatomically-realistic renal artery phantoms with varying low degrees of stenosis (0, 30 and 50%) were constructed and peak velocity data was measured from within the pre-stenotic and mid-stenotic regions in each phantom, for both unenhanced and contrast-enhanced spectral Doppler data acquisitions. The effect of a 20 mm overlying fat layer on the ultrasound beam distortion and phase aberration, and hence on the measured peak velocity data, was also investigated. RESULTS: The overlying fat layer produced a statistically significant underestimation (p < 0.01) in both the peak velocity and peak velocity ratio [Stenotic Region(Vmax)/Pre-stenotic Region(Vmax)] for the 0% and 30% stenosis models, but not the 50% model. A statistically significant increase (p < 0.01) in the peak velocity was found in the contrast-enhanced Doppler spectra; however, no significant difference was found between the unenhanced and contrast enhanced peak velocity ratio data, which suggests that the ratio metric has better diagnostic accuracy. The peak velocity ratios determined for each of the contrast-enhanced phantoms correctly predicted if the phantom had a stenosis and furthermore correctly classified the degree of stenosis. CONCLUSION: Contrast-enhanced Doppler ultrasound could significantly assist in the early detection of renal artery disease.

Investigation of the potential for sera from cattle persistently infected with bovine viral diarrhea virus to generate false-negative antibody ELISA results in pooled serum from seropositive and seronegative cattle.

We investigated the potential for viremic sera from cattle persistently infected with bovine viral diarrhea virus to create false-negative antibody results when testing pools of 10 sera by indirect or blocking ELISAs. Seronegative viremic sera ( n = 23) were each added to a series of artificially constructed pools containing various percentages (0-90%) of antibody-positive sera, and the resulting pools were assayed for antibody. In all 23 cases, a negative antibody result was obtained in the pool containing no seropositive sera. In contrast, all pools containing ≥10% seropositive serum, representing a single seropositive animal in a pool of 10 samples, returned a positive result in both antibody ELISAs. We concluded that the likelihood of a false-negative antibody result occurring as a result of the presence of serum from a viremic animal was low, and therefore did not preclude the use of pooled sera for serosurveillance.

A review of the recommendations governing quality assurance of ultrasound systems used for guidance in prostate brachytherapy.

Ultrasound guided brachytherapy for the treatment of prostate cancer has become a routine treatment option, due to many benefits including patient recovery and dose localisation [1]; however it is not clear whether the standards which govern the image quality for these systems are adequate. Upon review of the recommended standards for ultrasound systems used in prostate brachytherapy procedures, the recommended tests do not appear to be specific to the clinical application of ultrasound guided prostate brachytherapy. Rather they are generic and similar to those recommended for other clinical applications such as general abdominal scanning [2]. Furthermore, there is growing evidence that these tests should be specific to the clinical application [3,4] in order to gain meaningful data about the performance of the system for the application, and also to detect clinically relevant changes in quality control results. An additional problem is that there are no clinically relevant test phantom recommended for the quality assurance of ultrasound systems used in prostate brachytherapy. The image quality for this application of ultrasound needs to be monitored to ensure consistent levels of confidence in the procedure. This paper reviews the currently recommended test guidelines and test phantoms for ultrasound systems used in prostate brachytherapy from the different standard bodies and professional organisations. A critical analysis of those tests which are most reflective of the imaging and guidance tasks undertaken in an ultrasound guided prostate brachytherapy procedure will also be presented to inform the design of a TRUS quality assurance protocol.

Sperm-Coating Beta-Defensin 126 Is a Dissociation-Resistant Dimer Produced by Epididymal Epithelium in the Bovine Reproductive Tract.

Beta-defensins are innate immune molecules, often described as antimicrobial peptides because of their bactericidal activity and are now known to have diverse additional functions, including cell signaling, chemoattraction, immunoregulation, and reproduction. In humans and primates, beta-defensin 126 has been shown to regulate the ability of sperm to swim through cervical mucus and to protect sperm from attack by the female immune system during transit toward the oviduct. Bovine beta-defensin 126 (BBD126) is the ortholog of human defensin 126, and computational analysis here revealed significant conservation between BBD126 and other mammalian orthologs at the N-terminus, although extensive sequence differences were detected at the C-terminus, implying possible species-specific roles for this beta-defensin in reproduction. We had previously demonstrated preferential expression of this and related beta-defensin genes in the bovine male reproductive tract, but no studies of bovine beta-defensin proteins have been performed to date. Here, we analyzed BBD126 protein using a monoclonal antibody (a-BBD126) generated against a 14 amino acid peptide sequence from the secreted fragment of BBD126. The specificity of a-BBD126 was validated by testing against the native form of the peptide recovered from bovine caudal epididymal fluid and recombinant BBD126 generated using a prokaryotic expression system. Western blot analysis of the native and recombinant forms showed that BBD126 exists as a dimer that was highly resistant to standard methods of dissociation. Immunohistochemical staining using a-BBD126 demonstrated BBD126 protein expression by epithelial cells of the caudal epididymis and vas deferens from both mature and immature bulls. BBD126 could also be seen (by confocal microscopy) to coat caudal sperm, with staining concentrated on the tail of the sperm cells. This study is the first to demonstrate beta-defensin 126 protein expression in the bovine reproductive tract and on bull sperm. Its dissociation-resistant dimeric structure is likely to have important functional implications for the role of BBD126 in bovine reproduction.

Use of a radio frequency shield during 1.5 and 3.0 Tesla magnetic resonance imaging: experimental evaluation.

Radiofrequency (RF) shields have been recently developed for the purpose of shielding portions of the patient's body during magnetic resonance imaging (MRI) examinations. We present an experimental evaluation of a commercially available RF shield in the MRI environment. All tests were performed on 1.5 T and 3.0 T clinical MRI scanners. The tests were repeated with and without the RF shield present in the bore, for comparison. Effects of the shield, placed within the scanner bore, on the RF fields generated by the scanner were measured directly using tuned pick-up coils. Attenuation, by as much as 35 dB, of RF field power was found inside the RF shield. These results were supported by temperature measurements of metallic leads placed inside the shield, in which no measurable RF heating was found. In addition, there was a small, simultaneous detectable increase (∼1 dB) of RF power just outside the edges of the shield. For these particular scanners, the autocalibrated RF power levels were reduced for scan locations prescribed just outside the edges of the shield, which corresponded with estimations based on the pick-up coil measurements. Additionally, no significant heating during MRI scanning was observed on the shield surface. The impact of the RF shield on the RF fields inside the magnet bore is likely to be dependent on the particular model of the RF shield or the MRI scanner. These results suggest that the RF shield could be a valuable tool for clinical MRI practices.

Assessment of three methods for detection of ultrasound artifacts.

PURPOSE: The purpose of this work is to measure the performance (sensitivity and specificity) of three different visually based methods of detecting ultrasound artifacts using dynamic clips obtained with a liquid phantom. It is important to detect the presence of these non uniformities as early as possible, so they can be assessed, tracked, and addressed well before clinical image quality is impacted. METHODS: A total of 28 transducers of varying models containing a single artificial artifact created by stretching thin filaments across the transducer face were prepared. A second set of 28 matching transducers contained no artifacts. A 10 s clip was recorded of a dynamic speckle pattern from a custom liquid phantom ("dynamic clip"). A single-frame image was obtained by computing the median values at each pixel location over all frames of the clip ("median image"). This single-frame median image was then subtracted from a baseline image previously obtained with no induced artifact ("subtracted median"). All images were evaluated by six observers. The mean sensitivity and specificity with 95% exact binomial confidence intervals for the three artifact detection methods were estimated. Evaluation time and observer confidence were recorded. RESULTS: Both single-frame median and subtracted median images had a higher sensitivity than the dynamic clip. Overall the subtracted median images had the highest sensitivity of 97%, while maintaining a high specificity of 92%. Observers identified artifacts in the shortest time and with the highest confidence with this method. The subtracted median method removed the original structural variations and non-uniformities, and reduced the likelihood of false-positives. CONCLUSIONS: From the three methods assessed in this study, subtracted median images allow detection of artifacts with very good sensitivity and specificity, low image evaluation times, and a high degree of observer confidence, making these ideal for routine QC. For acceptance testing, where there are no previous baseline images available for subtraction, the use of median images is useful, although comparison with median images from different transducers of the same model and ∕ or comparison of impressions from multiple observers should be made to decrease the incidence of false-positive findings. If median and subtracted median images are not available, direct inspection of the dynamic B-mode clips is useful for acceptance testing and quality control, but with lower sensitivity and somewhat longer evaluation time.

Four-year experience with a clinical ultrasound quality control program.

Ultrasound (US) quality control (QC) program data over a 4-year period from more than 45 scanners and more than 265 transducers were reviewed to optimize the program in terms of efficiency and effectiveness. Our program included evaluations of mechanical integrity, image uniformity, distance measurement accuracy and maximum depth of penetration (DOP). We computed failure rates and fraction of failures detected by each test. A total of 187 equipment problems were identified. Average annual scanner component and transducer failure rates were 10.5% and 13.9%, respectively. The mechanical integrity and uniformity evaluations detected 25.1% and 66.3% of all failures, respectively. Those evaluations plus defects detected by sonographers accounted for 98.4% of all detected failures. DOP and distance measurement accuracy were not effective at detecting equipment failures. For routine US QC, we recommend quarterly mechanical integrity and uniformity assessments of all transducers. A scanner with five transducers could be tested in an estimated 30 min or less.

Comparative imaging study in ultrasound, MRI, CT, and DSA using a multimodality renal artery phantom.

PURPOSE: A range of anatomically realistic multimodality renal artery phantoms consisting of vessels with varying degrees of stenosis was developed and evaluated using four imaging techniques currently used to detect renal artery stenosis (RAS). The spatial resolution required to visualize vascular geometry and the velocity detection performance required to adequately characterize blood flow in patients suffering from RAS are currently ill-defined, with the result that no one imaging modality has emerged as a gold standard technique for screening for this disease. METHODS: The phantoms, which contained a range of stenosis values (0%, 30%, 50%, 70%, and 85%), were designed for use with ultrasound, magnetic resonance imaging, x-ray computed tomography, and x-ray digital subtraction angiography. The construction materials used were optimized with respect to their ultrasonic speed of sound and attenuation coefficient, MR relaxometry (T1, T2) properties, and Hounsfield number/x-ray attenuation coefficient, with a design capable of tolerating high-pressure pulsatile flow. Fiducial targets, incorporated into the phantoms to allow for registration of images among modalities, were chosen to minimize geometric distortions. RESULTS: High quality distortion-free images of the phantoms with good contrast between vessel lumen, fiducial markers, and background tissue to visualize all stenoses were obtained with each modality. Quantitative assessments of the grade of stenosis revealed significant discrepancies between modalities, with each underestimating the stenosis severity for the higher-stenosed phantoms (70% and 85%) by up to 14%, with the greatest discrepancy attributable to DSA. CONCLUSIONS: The design and construction of a range of anatomically realistic renal artery phantoms containing varying degrees of stenosis is described. Images obtained using the main four diagnostic techniques used to detect RAS were free from artifacts and exhibited adequate contrast to allow for quantitative measurements of the degree of stenosis in each phantom. Such multimodality phantoms may prove useful in evaluating current and emerging US, MRI, CT, and DSA technology.

Development of a vessel-mimicking material for use in anatomically realistic Doppler flow phantoms.

Polyvinyl alcohol cryogel (PVA-C) is presented as a vessel-mimicking material for use in anatomically realistic Doppler flow phantoms. Three different batches of 10% wt PVA-C containing (i) PVA-C alone, (ii) PVA-C with antibacterial agent and (iii) PVA-C with silicon carbide particles were produced, each with 1-6 freeze-thaw cycles. The resulting PVA-C samples were characterized acoustically (over a range 2.65 to 10.5 MHz) and mechanically to determine the optimum mixture and preparation for mimicking the properties of healthy and diseased arteries found in vivo. This optimum mix was reached with the PVA-C with antibacterial agent sample, prepared after two freeze/thaw cycles, which achieved a speed of sound of 1538 ± 5 m s(-1) and a Young's elastic modulus of 79 ± 11 kPa. This material was used to make a range of anatomically realistic flow phantoms with varying degrees of stenoses, and subsequent flow experiments revealed that higher degrees of stenoses and higher velocities could be achieved without phantom rupturing compared with a phantom containing conventional wall-less vessels.

Evaluation of a low-cost liquid ultrasound test object for detection of transducer artefacts.

Routine quality control of ultrasound scanners and transducers is important for maintaining image quality. Our experience suggests that artefact and uniformity evaluation is the most effective single phantom test for detecting equipment problems. Current methods for assessing ultrasound images for artefacts have important limitations. To overcome these limitations, we have developed a novel, low-cost, liquid phantom with a flexible surface for assessing artefacts. A range of materials were evaluated and the optimal liquid phantom was found to be a water/cornstarch solution contained within a flexible latex balloon. When compared to a rigid tissue-mimicking phantom no deficiencies in overall image appearance or artefact detection for any transducer model was observed for the liquid phantom. With minimal training, reproducible clips were obtained by clinical sonographers with low inter- and intra-operator dependence, for a range of transducers models. The flexible scanning surface of the liquid phantom allows complete rapid coupling of all transducers. Due to its ease of use and low cost this liquid phantom appears superior to rigid phantoms for assessment of non-uniformity artefacts, and should allow clinical practices to perform routine artefact assessments of all ultrasound scanners and transducers.

Development of a range of anatomically realistic renal artery flow phantoms.

Computer-aided modelling techniques were used to generate a range of anatomically realistic phantoms of the renal artery from medical images of a 64-slice CT data set acquired from a healthy volunteer. From these data, models of a normal healthy renal artery and diseased renal arteries with 30%, 50%, 70% and 85% stenoses were generated. Investment casting techniques and a low melting point alloy were used to create the vessels with varying degrees of stenosis. The use of novel inserts significantly reduced the time, materials and cost required in the fabrication of these anatomically realistic phantoms. To prevent residual metal remaining in the final phantom lumens a technique employing clingfilm was used to remove all molten metal from the lumen. These novel flow phantoms developed using efficient methods for producing vessels with various degrees of stenosis can provide a means of evaluation of current and emerging ultrasound technology.