COVID-19 vaccination intention and vaccine hesitancy among citizens of the Métis Nation of Ontario.

OBJECTIVE: The study objective is to measure the influence of psychological antecedents of vaccination on COVID-19 vaccine intention among citizens of the Métis Nation of Ontario (MNO). METHODS: A population-based online survey was implemented by the MNO when COVID-19 vaccines were approved in Canada. Questions included vaccine intention, the short version of the "5C" psychological antecedents of vaccination scale (confidence, complacency, constraint, calculation, collective responsibility), and socio-demographics. Census sampling via the MNO Registry was used achieving a 39% response rate. Descriptive statistics, bivariate analyses, and multinomial logistic regression models (adjusted for sociodemographic variables) were used to analyze the survey data. RESULTS: The majority of MNO citizens (70.2%) planned to be vaccinated. As compared with vaccine-hesitant individuals, respondents with vaccine intention were more confident in the safety of COVID-19 vaccines, believed that COVID-19 is severe, were willing to protect others from getting COVID-19, and would research the vaccines (Confident OR = 19.4, 95% CI 15.5-24.2; Complacency OR = 6.21, 95% CI 5.38-7.18; Collective responsibility OR = 9.83, 95% CI 8.24-11.72; Calculation OR = 1.43, 95% CI 1.28-1.59). Finally, respondents with vaccine intention were less likely to let everyday stress prevent them from getting COVID-19 vaccines (OR = 0.47, 95% CI 0.42-0.53) compared to vaccine-hesitant individuals. CONCLUSION: This research contributes to the knowledge base for Métis health and supported the MNO's information sharing and educational activities during the COVID-19 vaccines rollout. Future research will examine the relationship between the 5Cs and actual uptake of COVID-19 vaccines among MNO citizens.

RéSUMé: OBJECTIF: Nous avons cherché à mesurer l'influence des antécédents psychologiques de vaccination sur l'intention de se faire vacciner contre la COVID-19 chez les citoyennes et citoyens de la Nation métisse de l'Ontario (NMO). MéTHODE: Un sondage populationnel en ligne a été mis en œuvre par la NMO quand des vaccins contre la COVID-19 ont été approuvés au Canada. Les questions posées ont porté sur l'intention de se faire vacciner, la version abrégée du modèle « 5C ¼ de l'échelle de vaccination (Confiance, Contraintes, Complaisance, Calcul et responsabilité Collective) et le profil sociodémographique. Nous avons utilisé l'échantillonnage fondé sur le recensement via le registre de la NMO pour obtenir un taux de réponse de 39 %. Des statistiques descriptives, des analyses bivariées et des modèles de régression logistique multinomiale (ajustés selon les variables sociodémographiques) ont servi à analyser les données du sondage. RéSULTATS: La majorité (70,2 %) des citoyennes et citoyens de la NMO prévoyaient se faire vacciner. Comparativement aux personnes réticentes à l'égard de la vaccination, les personnes ayant l'intention de se faire vacciner avaient plus confiance en l'innocuité des vaccins contre la COVID-19, considéraient la COVID-19 comme une maladie grave, étaient disposées à protéger les autres contre la COVID-19 et cherchaient à se renseigner au sujet des vaccins (Confiance : RC = 19,4, IC95% 15,5­24,2; Complaisance : RC = 6,21, IC95% 5,38­7,18; responsabilité Collective : RC = 9,83, IC95% 8,24­11,72; Calcul : RC = 1,43, IC95% 1,28­1,59). Enfin, les répondantes et les répondants ayant l'intention de se faire vacciner étaient moins susceptibles de laisser le stress quotidien les empêcher de se faire vacciner contre la COVID-19 (RC = 0,47, IC95% 0,42­0,53) comparativement aux personnes réticentes à l'égard de la vaccination. CONCLUSION: Cette étude contribue à la base de connaissances sur la santé des Métis et a appuyé les activités de sensibilisation et d'échange d'informations de la NMO pendant le déploiement des vaccins contre la COVID-19. Une étude future portera sur la relation entre les « 5C ¼ et le recours réel aux vaccins contre la COVID-19 chez les citoyennes et citoyens de la NMO.

COVID-19 vaccine behaviour among citizens of the Métis Nation of Ontario: A qualitative study.

BACKGROUND: The burden of the current COVID-19 pandemic is not shared equally in Canadian society, with Indigenous Peoples being disproportionately affected. Moreover, there is a lack of research pertaining to vaccination behaviour in Métis communities. This Métis-specific and Métis-led qualitative study endeavours to understand COVID-19 vaccine behaviour among citizens of the Métis Nation of Ontario (MNO). METHODS: Data was collected via one-on-one interviews. Participants were recruited via the MNO's existing social media channels. Participants filled out a screening survey indicating their intention to vaccinate against COVID-19 as yes, no, or unsure. Sixteen participants (9 yes, 3 unsure, 4 no) were interviewed. Interviews averaged 30 min, and the questions and probes were developed in collaboration with the MNO. The interviewer received Métis-specific cultural safety training. Interviews were transcribed verbatim and uploaded to NVivo 12. RESULTS: A deductive analysis using the Social Ecological Model framework (SEM) for vaccine behaviour and two blinded coders was used to understand the data. An additional factor, COVID-19 public health measures, was added to the framework to better capture the experiences of participants during the COVID-19 pandemic. Overall, the factors with the greatest number of coded references included Vaccine roll-out and availability, Organization of the public into priority groups, Public discourse, Interpersonal influences, Interface with health professionals, Knowledge state, Trust, and Vaccine risk perception. Bandwagoning (following others' behaviour) and Freeloading (perceiving enough people have been vaccinated), both factors of the SEM, were not discussed. Yes, no, and unsure participant groups were compared to understand the influences of each factor based on COVID-19 vaccination intention. CONCLUSIONS: MNO citizens COVID-19 vaccine behaviour was negatively and positively influenced by a number of factors. This information will allow the MNO and public health units to better tailor their messaging for COVID-19 vaccine uptake campaigns and future pandemic emergencies.

Inclusion of intersectionality in studies of immunization uptake in Canada: A scoping review.

INTRODUCTION: Intersectionality refers to the interconnectedness of various social locations creating unique experiences for individuals and groups, in the context of systems of privilege and oppression. As part of immunization coverage research, intersectionality allows healthcare professionals and policymakers to become aware of the constellation of characteristics contributing to low vaccine uptake. The objective of this study was to examine the application of intersectionality theory or concepts, and the appropriate use of sex and gender terminology, in Canadian immunization coverage research. MATERIALS AND METHODS: The eligibility criteria for this scoping review included English or French language studies on immunization coverage among Canadians of all ages. Six research databases were searched without date restrictions. We searched provincial and federal websites, as well as the Proquest Dissertations and Theses Global database for grey literature. RESULTS: Of 4725 studies identified in the search, 78 were included in the review. Of these, 20 studies included intersectionality concepts, specifically intersections of individual-level characteristics influencing vaccine uptake. However, no studies explicitly used an intersectionality framework to guide their research. Of the 19 studies that mentioned "gender", 18 had misused this term, conflating it with "sex". CONCLUSIONS: Based on our findings, there is an evident lack of intersectionality framework utilization in immunization coverage research in Canada, as well as misuse of the terms "gender" and "sex". Rather than only focusing on discrete characteristics, research should explore the interaction between numerous characteristics to better understand the barriers to immunization uptake in Canada.

Improved vaccine coverage for First Nations children receiving first dose on-reserve: a retrospective cohort study in western Canada.

INTRODUCTION: Fragmentation in immunisation reporting systems pose challenges in measuring vaccine coverage for First Nations children in Canada. Some Nations have entered into data-sharing agreements with the province of Alberta's health ministry, enabling novel opportunities to calculate coverage. METHODS: Partnering with a First Nations community in Alberta, this retrospective cohort study calculated routine childhood vaccine coverage. Administrative data for vaccines delivered within and outside the community were linked to calculate partial and complete immunisation coverage in 2013-2019 at ages 2 and 7 years for children living in the community. We also compared vaccine coverage each year for (a) children who were and were not continuous community residents and (b) children who received or not their first vaccine at the on-reserve community health centre. We also calculated the mean complete coverage across all study years with 95% CIs. RESULTS: For most vaccines, coverage was higher (p<0.05) at ages 2 and 7 years for children that received their first vaccine at the First Nations health centre, compared with those who received their first dose elsewhere. For example, for pneumococcal vaccine, the mean level of complete coverage in 2-year-olds was 55.7% (52.5%-58.8%) for those who received their first vaccine in the community, compared with 33.3% (29.4%-37.3%) for those who did not; it was also higher at 7 years (75.6%, 72.7%-78.5%, compared with 55.5%, 49.7%-61.3%). CONCLUSION: Initiating the vaccine series at the on-reserve community health centre had a positive impact on coverage. The ability to measure accurate coverage through data-sharing agreements and vaccine record linkage will support First Nations communities in identifying individual and community immunity. The findings also support the transfer of health funding and service delivery to First Nations to improve childhood immunisation uptake.

Practice report: an Alberta Métis model for COVID-19 vaccine delivery.

SETTING: In January 2021, the COVID-19 vaccine became available to First Nations, Métis, and Inuit (FNMI) over the age of 65 living in First Nations communities or Métis settlements in Alberta. In March, vaccine eligibility in Alberta expanded to include FNMI peoples of younger ages and in urban settings. The Métis Nation of Alberta (MNA) and other Indigenous organizations recognized that FNMI populations might be better served by tailored vaccine programs. INTERVENTION: The MNA is the government for the Métis people in Alberta. During the COVID-19 pandemic, the MNA has supported its citizens, through financial and mental wellness support, access to personal protective equipment, and messaging regarding public health orders. When vaccines became available, culturally appropriate virtual vaccine information sessions were provided. In March 2021, the MNA delivered the first Métis-led COVID-19 vaccination clinic. Unique to the clinic's success was the location, online booking process, and community presence. The clinic focused on cultural safety, including the availability of Indigenous health professionals to community members, and cultural reference points throughout the clinic. OUTCOMES: In the first MNA clinic, over 1300 people were vaccinated. Visitors shared appreciation for the culturally specific aspects of the clinic, which contributed to increased safety and comfort. IMPLICATIONS: Based on the success of the first Métis-led vaccination clinic, similar services in communities with high numbers of Métis people have been approved. This innovative practice initiative could provide a model of COVID-19 vaccine service delivery that could be used to meet the needs of Métis citizens in other jurisdictions in Canada.

RéSUMé: LIEU: En janvier 2021, le vaccin anti-COVID-19 est devenu accessible aux Premières Nations, Inuits et Métis (PNIM) de 65 ans et plus vivant dans les communautés des Premières nations ou les établissements métis de l'Alberta. En mars, l'admissibilité au vaccin en Alberta a été étendue aux personnes des PNIM plus jeunes et vivant en milieu urbain. La Nation métisse de l'Alberta (NMA) et d'autres organismes autochtones ont pensé que les populations PNIM seraient peut-être mieux servies par des programmes de vaccination adaptés. INTERVENTION: La NMA est le gouvernement des Métis en Alberta. Durant la pandémie, la NMA a appuyé ses citoyens en leur offrant des mesures d'aide financière et de bien-être mental, en leur donnant accès à de l'équipement de protection individuelle et en diffusant des messages sur les ordonnances de santé publique. Quand les vaccins sont devenus disponibles, des séances d'information virtuelles culturellement appropriées ont été offertes. En mars 2021, la NMA a monté la première clinique de vaccination contre la COVID-19 dirigée par des Métis. Le succès de cette clinique a reposé sur son emplacement, sur le processus de prise de rendez-vous et sur la présence communautaire. La clinique a mis l'accent sur la sécurisation culturelle, notamment en mettant à la disposition des résidents des professionnels de la santé autochtones et en plaçant des références culturelles à plusieurs endroits. RéSULTATS: Au cours de la première clinique de la NMA, plus de 1 300 personnes se sont fait vacciner. Les visiteurs ont dit apprécier les aspects culturellement spécifiques de la clinique, qui ont contribué à en accroître la sécurité et le confort. CONSéQUENCES: Avec le succès de la première clinique de vaccination dirigée par des Métis, des services semblables ont été approuvés dans des communautés où les Métis sont nombreux. Cette initiative novatrice pourrait être un modèle de prestation de services de vaccination contre la COVID-19 adaptés aux besoins des citoyens de la Nation métisse dans d'autres provinces et territoires du Canada.

Growth dynamics and the proximate biochemical composition and fatty acid profile of the heterotrophically grown diatom Cyclotella cryptica.

To investigate the nutritional value of the diatom Cyclotella cryptica as an alternative feed for aquaculture, its heterotrophic growth characteristics were studied. First, the proximate biochemical composition and fatty acid profiles were studied under a controlled heterotrophic growth condition. The approximate total ash, carbohydrate, lipid, and protein content were 245 mg g(-1) (dry weight), 360 mg g(-1), 165 mg g(-1) and 260 mg g(-1), respectively. Polyunsaturated fatty acids accounted for 24.5, 31.3, 45.1 and 17.3% of the total lipids in the phospholipid, sterol, free fatty acid and triglyceride classes. Secondly, the effect of aeration and agitation rates on the specific growth rate of C. cryptica under heterotrophic conditions was studied. The maximum specific growth rate was not significantly affected (P > 0.05) by the rate of agitation within the range of 100 to 160 rpm, but it was significantly affected (P > 0.05) by the rate of aeration. Optimal growth occurred when the aeration rate was within the range of 0.44 to 1.07 v/v/min. Viability measurements throughout the growth period showed that the C. cryptica cells remained viable in spite of the varied cultivation conditions. Hydrodynamic forces are an important parameter within biological systems, and optimisation is crucial for the successful scale-up of microalgal cultivation systems. Whilst the investigation was preliminary in nature, the information gained in this study will be useful for the continual development of an alternative and cost-effective feed for bivalve spat rations.

Heterotrophic growth and nutritional aspects of the diatom Cyclotella cryptica (Bacillariophyceae): Effect of some environmental factors.

To investigate the nutritional value of the diatom Cyclotella cryptica (Reimann, Lewin, and Guillard) as an alternative feed for use in the aquaculture industry, the heterotrophic growth characteristics and resultant fatty acid profile of the microalga were studied when cultivated under a variety of controlled salinity and temperature conditions. In addition, the effects of pH on the growth characteristics were also studied. The maximum specific growth rate was affected by initial pH and cultivation temperature, but not by salinity. The optimal pH and temperature ranges for growth were 7.2 to 8.1 and 22.5 to 25.0 degrees C, respectively. Lipid accumulation and the fatty acid composition were also affected by cultivation temperature and salinity. The optimal temperature range and salinity level for lipid accumulation were 18.0 to 25.0 degrees C and 11.2 psu, respectively. In all cases the fatty acid distribution was similar, with the most abundant fatty acids being palmitic acid (16:0), palmitoleic acid (16:1 n-7), stearidonic acid (18:4 n-3, SDA), eicosapentaenoic acid (20:5 n-3, EPA), and decosahexaenoic acid (22:6 n-3, DHA).

CF3CH3 --> HF + CF2CH2: a non-RRKM reaction?

The experimental shock tube data recently reported by Kiefer et al. [J. Phys. Chem. A 2004, 108, 2443-2450] for the title reaction at temperatures between 1600 and 2400 K have been compared to master equation simulations using three models: (a) standard RRKM theory, (b) RRKM theory modified by local random matrix theory, which introduces dynamical corrections arising from slow intramolecular vibrational energy randomization, and (c) an ad hoc empirical non-RRKM model. Only the third model provides a good fit of the Kiefer et al. unimolecular reaction rate data. In separate simulations, all three models accurately reproduce the experimental 300 K chemical activation data of Marcoux and Setser [J. Phys. Chem. 1978, 82, 97-108] when the energy transfer parameters are freely varied to fit the data. When experimental energy transfer parameters for a geometrical isomer (1,1,2-trifluoroethane) are used, the standard RRKM model fits the chemical activation data better than the other models, but if energy transfer in the 1,1,1-trifluoroethane is significantly reduced in comparison to the 1,1,2 isomer, then the empirical ad hoc non-RRKM model also gives a good fit. While the ad hoc empirical non-RRKM model can be made to fit the data, it is not based on theory, and we argue that it is physically unrealistic. We also show that the master equation simulations can mimic the Kiefer et al. vibrational relaxation data, which was the first shock tube observation of double-exponential relaxation. We conclude that, until more data on the trifluoroethanes become available, the current evidence is insufficient to decide with confidence whether non-RRKM effects are important in this reaction, or whether the Kiefer et al. data can be explained in some other way.