1.
Org Lett
; 2(9): 1307-10, 2000 May 04.
Artigo
em Inglês
| MEDLINE
| ID: mdl-10810734
RESUMO
[formula: see text] The enzyme S-adenosylmethionine:tRNA ribosyltransferase-isomerase (QueA) catalyzes the penultimate step in the biosynthesis of the tRNA nucleoside queuosine, a unique ribosyl transfer from the cofactor S-adenosylmethionine (AdoMet) to a modified-tRNA precursor. The use of AdoMet in this way is fundamentally new to the chemistry of this important biological cofactor. We report here the first mechanistic studies of this remarkable enzyme, and we propose a chemical mechanism for the reaction consistent with our experimental observations.
Assuntos
Pentosiltransferases/química , Pentosiltransferases/metabolismo , S-Adenosilmetionina/química , Isomerases , Espectroscopia de Ressonância Magnética , Nucleosídeo Q/biossíntese , RNA de Transferência/metabolismo , Ribose/metabolismo
2.
Can Fam Physician
; 45: 633-4, 1999 Mar.
Artigo
em Inglês
| MEDLINE
| ID: mdl-10099802