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1.
EJIFCC ; 24(1): 14-20, 2013 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-27683434

RESUMO

Opportunities to advance one's knowledge and position are available within the clinical laboratory arena. By obtaining a specialist credential in chemistry, hematology or microbiology, a laboratorian has demonstrated advance knowledge and ability in their respective discipline. These specialist certifications open doors within and outside the laboratory profession and may lead to promotion. The specialist in blood banking credential is unique in that accredited training programs are available, some of which are affiliated with universities and graduate credit is granted for program completion. Other avenues available include pathologist assistants programs, diplomats in laboratory management and Master of Science degrees in clinical laboratory science. There are a number of choices available to achieve your professional goal.

2.
Adv Skin Wound Care ; 20(6): 331-45, 2007 Jun.
Artigo em Inglês | MEDLINE | ID: mdl-17538259

RESUMO

OBJECTIVES: To evaluate cytotoxicity and bactericidal effects of chloramine-T. METHODS: In vitro study of various concentrations and exposure times to preparations containing human fibroblasts or 1.5 x 10 colony forming units per milliliter (CFU/mL) of 3 gram-positive bacteria-Staphylococcus aureus, methicillin-resistant S aureus, and vancomycin-resistant Enterococcus faecalis-and 2 gram-negative bacteria-Escherichia coli and Pseudomonas aeruginosa-with and without fetal bovine serum present. MAIN OUTCOME MEASURES: Percentage reduction of bacterial growth and percentage of viable fibroblasts 48 hours after exposure. RESULTS: All gram-positive growth was reduced by 95% to 100%, regardless of dose, with or without serum. E coli (gram-negative; with/without serum) was reduced 94% to 100% at antiseptic concentrations of 300 and 400 ppm. At 200 ppm, E coli growth was fully inhibited without serum present and by 50% with serum. P aeruginosa (gram-negative) was not significantly affected under any conditions. At 100 and 200 ppm, cell viability remained greater than 90% under all experimental conditions. A 300-ppm, 3-minute exposure to chloramine-T resulted in cell viability of up to 70%, with longer exposures producing lower viabilities. Serum did not affect cell viability in any condition. CONCLUSIONS: In vitro, chloramine-T at 200 ppm for 5 to 20 minutes was effective against 3 virulent gram-positive bacteria without fibroblast damage. At 300 ppm and 3 and 5 minutes, 30% of fibroblasts were damaged and 95% to 100 % of E coli were inhibited, respectively.


Assuntos
Anti-Infecciosos Locais/uso terapêutico , Infecções Bacterianas/tratamento farmacológico , Cloraminas/uso terapêutico , Fibroblastos/efeitos dos fármacos , Compostos de Tosil/uso terapêutico , Infecção dos Ferimentos/tratamento farmacológico , Anti-Infecciosos Locais/química , Anti-Infecciosos Locais/farmacologia , Infecções Bacterianas/microbiologia , Técnicas de Cultura de Células , Sobrevivência Celular/efeitos dos fármacos , Cloraminas/química , Cloraminas/farmacologia , Contagem de Colônia Microbiana , Avaliação Pré-Clínica de Medicamentos , Enterococcus faecalis , Infecções por Escherichia coli/tratamento farmacológico , Fibroblastos/ultraestrutura , Infecções por Bactérias Gram-Positivas/tratamento farmacológico , Humanos , Resistência a Meticilina , Testes de Sensibilidade Microbiana , Infecções por Pseudomonas/tratamento farmacológico , Pseudomonas aeruginosa , Infecções Estafilocócicas/tratamento farmacológico , Staphylococcus aureus , Fatores de Tempo , Compostos de Tosil/química , Compostos de Tosil/farmacologia , Resistência a Vancomicina , Infecção dos Ferimentos/microbiologia
3.
Clin Lab Sci ; 18(3): 150-9, 2005.
Artigo em Inglês | MEDLINE | ID: mdl-16134475

RESUMO

OBJECTIVE: To evaluate clinical laboratory science (CLS) student attitudes toward teamwork when using cooperative learning (CL) as compared to individual learning (IL) in a course and to determine if learning method affects student attitudes toward the course itself. DESIGN/SETTING/PARTICIPANTS: This was a multi-institutional study involving eight classrooms in seven states. The effects of CL and IL on student attitudes were compared for 216 student participants. INTERVENTION: One group of students learned the course material through a CL approach while a second group of students learned via a traditional IL approach. For each course, the instructor, class material, and examination content was identical for the CL and IL students; the only variable was learning method. MAIN OUTCOME MEASURE: Student attitudes toward teamwork and toward the course were evaluated with a 35-item Attitude Questionnaire administered as a posttest. Mean scores for the CL and IL groups were compared using the Student t-test for independent samples. RESULTS: No significant difference was seen between the CL and IL students when assessing the first 30 questions on student attitudes toward teamwork (means = 98.42 and 98.22, respectively) when all institutions were combined. Comparable results were seen for each of the eight institutions. For the five questions comparing attitudes toward the course itself, there usually was no significant difference in attitude between CL and IL students. The only classrooms where CL students had more positive attitudes were those with instructors who had more than 10 years experience with CL. CONCLUSION: Results suggest that CL produces similar student attitudes toward teamwork and toward a CLS course as does IL.


Assuntos
Química Clínica/educação , Comportamento Cooperativo , Laboratórios , Pessoal de Laboratório Médico/educação , Pessoal de Laboratório Médico/psicologia , Estudantes de Ciências da Saúde/psicologia , Atitude , Humanos , Individualidade
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