[Immunoenzyme demonstration of viruses and virus-specific antibodies by using conjugates based on gene engineering-produced beta-lactamase]. / Immunofermentnaia indikatsiia virusov i virusspetsificheskikh antitel s isol'zovaniem kon''iugatov na osnove beta-laktamazy, poluchennoi genno-inzhenernym sposobom.

Enzyme immunoassays for the detection of viral antigens and virus-specific antibodies in biological samples have been described. Molecular complexes of antibodies and beta-lactamase (penicillinase) have been used as anti-specific conjugates. To synthesize the conjugate, the enzyme obtained with the aid of genetic engineering has been used. Enzyme immunoassays have been tested for the indication of the influenza virus and virus-induced specific antibodies. Enzyme immunoassays were shown to possess certain advantages (e.g., the use of simple and nontoxic substrate) along with the sensitivity identical to that of other methods, employing peroxidase-based conjugates.

[The effect of exogenous RNA and their fragments on yeast beta-galactosidase activity]. / Deistvie ékzogennykh RNK i ikh fragmentov na aktivnost' beta-galaktozidazy drozhzhei.

It is shown that a degradation level of exogenic high-polymeric RNA does not change their stimulating effect on beta-galactosidase synthesis in yeast protoplasts. Nucleoside monophosphates are a product of RNA degradation which promotes an increase in the beta-galactosidase activity. The exogenic RNA is established to act at the transcription level. RNA and its degradation products stimulate the beta-galactosidase synthesis without any effect on the intensity of the total RNA and protein synthesis in the yeast protoplasts. All mentioned permitted a conclusion that products of RNA decay participate in the regulation of the beta-galactosidase synthesis of yeast. A possible mechanism of their action is under discussion.