RESUMO
Background: The regeneration of lost/damaged support tissue in the periodontium, including the alveolar bone, periodontal ligament, and cementum, is an ambitious purpose of periodontal regenerative therapy and might effectively reduce periodontitis-caused tooth loss. Guided tissue regeneration (GTR) is a technique currently used in dentistry for periodontal surgery, which allows osseous regeneration prior to soft tissue migration into the area of interest. Calcium phosphate-based bone grafts (mostly Tricalcium Phosphate or Hydroxyapatite) are bio ceramics that show the greatest similarity to the mineral found in the bone. Thereby, giving calcium-phosphate excellent biocompatibility, biodegradability and osteoconductivity. The aim of the study is to fabricate hydroxyapatite reinforced polymeric hydrogel membrane for regeneration. Materials and Method: Pure alginate fabrication was done by cross linking sodium alginate with calcium chloride. Hydroxyapatite (HAP) alginate (Alg) was formulated by adding nanoparticles to the alginate mixture, which was then cross-linked with calcium chloride to formulate a HAP alginate polymeric membrane. The Fourier-transform infrared spectroscopy (FT-IR), Scanning Electron Microscope (SEM), and biocompatibility tests were performed to analyse the membrane characteristics. Results: Fabricated Hydroxyapatite- alginate (Hap- Alg) membrane has longer durability, because of strong crystal structure which in turn might take a longer time to regenerate. The membrane was found to be biocompatible and HAp induces faster mineralisation which in turn will increase the tissue regeneration rate of the membrane. Conclusion: The findings of our study suggests that the HAP-Alg hydro gel membrane is highly durable and hemocompatible and it has faster mineralisation capability thus making it superior from the clinically available membranes for GTR. Further analyses needs to be conducted to evaluate the potential of this membrane to be used for regeneration.
RESUMO
Pit-and-fissure sealants of the occlusal surfaces for the posterior teeth are well susceptible to develop dental caries compared to the smooth surfaces due to its complex morphology, which makes dental hygiene a challenging fact which give rise to increased plaque accumulation. The use of pit-and-fissure sealants is to provide inhibition toward microorganisms and aggregation of food particles, thereby inhibiting caries initiating and preventing caries progression. The study aimed to assess the effect of pit-and-fissure sealants postbrushing simulation through photography. For this photogenic original study, eight teeth were selected, with two groups each and four teeth present in each of the groups. Brushing simulator machines are well-established methods for analyzing the efficacy of toothbrushes. A total of 10,000 cycles were accounted for by the machine with linear X-axis and Y-axis = 2500 in each group. The clockwise and counterclockwise = 2500 in each group. From the research, it can be concluded that Group A is found to be more effective than Group B, where Group A is Clinpro sealant and Group B is YuSeal. This pit-and-fissure sealant application is very important in clinical practice to prevent the tooth from getting decayed. This research helps us to establish the amount of reduction in both the groups of sealants postbrushing as various brushing patterns influence the retention of pit-and-fissure sealants.
