Assuntos
Aberrações Cromossômicas , Leucemia/genética , Adulto , Idoso , Feminino , Humanos , Cariotipagem , Masculino , Pessoa de Meia-Idade , Linfócitos TRESUMO
Asymptomatic, female, 56-year-old identical Japanese twins were found to have a severe abnormality in the surface-mediated intrinsic coagulation, fibrinolysis and esterolytic activity. These defects were thought to be due to the lack of Fitzgerald factor, because of the prolongations of kaolin-activated partial thromboplastin time and kaolin-activated euglobulin lysis time that were not corrected by the addition of Fitzgerald trait-plasma but were corrected to normal levels by the addition of isolated bovine high-molecular-weight kininogen, Fletcher trait-plasma or Hageman trait-plasma.
Assuntos
Transtornos da Coagulação Sanguínea/epidemiologia , Animais , Transtornos da Coagulação Sanguínea/tratamento farmacológico , Testes de Coagulação Sanguínea , Bovinos , Deficiência do Fator XI/sangue , Deficiência do Fator XII/sangue , Feminino , Humanos , Japão , Cininogênios/uso terapêutico , Pessoa de Meia-Idade , Tempo de Tromboplastina Parcial , Linhagem , Pré-Calicreína/metabolismoAssuntos
Nucleotídeos de Adenina/sangue , Transtornos Plaquetários/sangue , Plaquetas/análise , Glicoproteínas/sangue , Proteínas de Membrana/sangue , Trombocitopenia/sangue , Difosfato de Adenosina/sangue , Adenosina Trifosfatases/sangue , Adulto , Membrana Celular/análise , Feminino , Humanos , MasculinoRESUMO
A coil planet centrifuge (CPC) is an apparatus aiming to obtain a centrifugal force exerting on particles suspended in a fluid in a coiled polyethylene tube (coil) which makes self-rotation around its axis and revolution around the principal axis simultaneously. If a linear osmotic gradient is prepared in the coil and a small amount of blood is applied to it and subjected to centrifugation, one is able to observe the membrane properties of erythrocytes in terms of the shift of hemolysis band appearing in the coil with high accuracy and precision. Coil planet centrifugation was applied to the observation of altered membrane properties of erythrocytes in hepatobiliary diseases. Remarkable shifts of end-point of the hemolysis band going in parallel with the severity of damage were visualized. Study as to its cause revealed that increased free cholesterol content of erythrocyte membrane which was the result of plasma cholesterol Esterstürz due to inhibition of lecithin-cholesterol acyltransferase by stagnant bile acids was responsible for this phenomenon. Elevation of serum bile acid concentration and increased serum leucine-aminopeptidase were closely correlated to the shift of hemolysis end-point toward the low osmolal side. CPC may be useful for detection and chasing the clinical course of patients with hepatobiliary disorders.
