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1.
Food Sci Biotechnol ; 30(11): 1417-1425, 2021 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-34790425

RESUMO

BARLEYmax, a barley variety, and cocoa polyphenols (CPPs) have been reported to affect bacterial metabolites in the colon. This study aimed to evaluate the combined effects of BARLEYmax and CPPs supplementation on fecal microbiota in vitro using pig feces for 48 h. The relative abundances of the family Clostridiaceae and the genus Clostridium and ammonia-nitrogen production were decreased by both BARLEYmax and CPP supplementation, and there was a positive correlation between their abundances and the ammonia-nitrogen concentration. Although acetate and n-butyrate production was decreased by CPP supplementation, their concentrations were maintained at a higher level in the BARLEYmax + CPP group than in the cellulose (control) and cellulose + CPP groups. Therefore, this study demonstrated that a combination of BARLEYmax and CPPs may be beneficial in maintaining higher short-chain fatty acid production and the elimination of potentially harmful factors. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s10068-021-00959-z.

2.
Int J Biol Macromol ; 189: 151-159, 2021 Oct 31.
Artigo em Inglês | MEDLINE | ID: mdl-34400230

RESUMO

Gut microbial fermentation of soluble dietary fibers promotes general and substrate-specific health benefits. In this study, the fermentation characteristics of two soluble branched-dietary fibers, namely, agavin (a type of agave fructans) and digestion-resistant maltodextrin (RD) were investigated against cellulose, using a simulated colonic fermenter apparatus employing a mixed culture of swine fecal bacteria. After 48 h of complete fermentation period, the microbial composition was different among all groups, where Bifidobacterium spp. and Lactobacillus spp. dominated the agavin treatment, while the members of the families Lachnospiraceae and Prevotellaceae dominated the RD treatment. Agavin treatment exhibited a clearly segregated two-phased prolonged fermentation trend compared to RD treatment as manifested by the fermentation rates. Further, the highest short-chain fatty acids production even at the end of the fermentation cycle, acidic pH, and the negligible concentration of ammonia accumulation demonstrated favorable fermentation attributes of agavin compared to RD. Therefore, agavin might be an effective and desirable substrate for the colonic microbiota than RD with reference to the expressed microbial taxa and fermentation attributes. This study revealed a notable significance of the structural differences of fermentable fibers on the subsequent fermentation characteristics.


Assuntos
Técnicas de Cultura Celular por Lotes , Colo/fisiologia , Fermentação , Frutanos/metabolismo , Polissacarídeos/metabolismo , Amônia/análise , Animais , Bifidobacterium/crescimento & desenvolvimento , Biodiversidade , Contagem de Colônia Microbiana , Digestão , Ácidos Graxos Voláteis/metabolismo , Concentração de Íons de Hidrogênio , Lactobacillus/crescimento & desenvolvimento , Microbiota , Nitrogênio/análise , Filogenia , Suínos , Fatores de Tempo
3.
Biosci Biotechnol Biochem ; 84(7): 1486-1496, 2020 Jul.
Artigo em Inglês | MEDLINE | ID: mdl-32281519

RESUMO

Inulin-type fructans are known to exert different effects on the fermentation profile depending on the average and range of the degree of polymerization (DP). Here, swine fecal cultures were used to investigate the prebiotic properties of native chicory inulin (NIN), extracted from the chicory root, and synthetic inulin (SIN), which has a narrower DP distribution than NIN. Both NIN and SIN showed prebiotic effects, but NIN exhibited a significant decrease in pH and increase in the production of propionate and butyrate compared to SIN. There were also differences in the production of succinate and lactate, the precursors of propionate and butyrate, and the relative abundance of associated genes. Furthermore, NIN induced the growth of certain species of Bifidobacterium and Lactobacillus more strongly than SIN. These results suggest that NIN and SIN exhibit different prebiotic properties due to differences in DP, and that NIN might be more beneficial to host health.


Assuntos
Cichorium intybus/química , Fezes/microbiologia , Inulina/farmacologia , Extratos Vegetais/farmacologia , Raízes de Plantas/química , Prebióticos , Animais , Bifidobacterium/efeitos dos fármacos , Bifidobacterium/crescimento & desenvolvimento , Butiratos/metabolismo , Fermentação , Inulina/síntese química , Inulina/química , Lactobacillus/efeitos dos fármacos , Lactobacillus/crescimento & desenvolvimento , Extratos Vegetais/química , Polimerização , Propionatos/metabolismo , Suínos
4.
PLoS One ; 14(6): e0218118, 2019.
Artigo em Inglês | MEDLINE | ID: mdl-31185060

RESUMO

We investigated whether supplementation with the barley line BARLEYmax (Tantangara; BM), which contains three fermentable fibers (fructan, ß-glucan, and resistant starch), modifies the microbiota in cecal and distal colonic digesta in addition to short-chain fatty acids (SCFAs) production more favorably than supplementation with a high-ß-glucan barley line (BG012; BG). Male Sprague-Dawley rats were randomly divided into 3 groups that were fed an AIN-93G-based diet that contained 5% fiber provided by cellulose (control), BM or BG. Four weeks after starting the respective diets, the animals were sacrificed and digesta from the cecum, proximal colon and distal colon were collected and the SCFA concentrations were quantified. Microbiota in the cecal and distal colonic digesta were analyzed by 16S rRNA sequencing. The concentrations of acetate and n-butyrate in cecal digesta were significantly higher in the BM and BG groups than in the control group, whereas the concentration of total SCFAs in cecal digesta was significantly higher only in the BM group than in the control group. The concentrations of acetate and total SCFAs in the distal colonic digesta were significantly higher only in the BM group than in the control group. The abundance of Bacteroidetes in cecal digesta was significantly higher in the BM group than in the control group. In contrast, the abundance of Firmicutes in cecal digesta was significantly lower in the BM and BG groups than in the control group. These results indicated that BM increased the concentration of total SCFAs in the distal colonic digesta. These changes might have been caused by fructan and resistant starch in addition to ß-glucan. In conclusion, fermentable fibers in BM reached the distal colon and modified the microbiota, leading to an increase in the concentration of total SCFAs in the distal colonic digesta, more effectively compared with the high-ß-glucan barley line (BG).


Assuntos
Bacteroidetes/metabolismo , Ceco/microbiologia , Colo/microbiologia , Ácidos Graxos/biossíntese , Hordeum , Microbiota/efeitos dos fármacos , beta-Glucanas/farmacologia , Ração Animal , Animais , Ratos , Ratos Sprague-Dawley
5.
Microb Cell Fact ; 11: 49, 2012 Apr 30.
Artigo em Inglês | MEDLINE | ID: mdl-22545774

RESUMO

BACKGROUND: Benzoic acid is one of the most useful aromatic compounds. Despite its versatility and simple structure, benzoic acid production using microbes has not been reported previously. Streptomyces are aerobic, Gram-positive, mycelia-forming soil bacteria, and are known to produce various kinds of antibiotics composed of many aromatic residues. S. maritimus possess a complex amino acid modification pathway and can serve as a new platform microbe to produce aromatic building-block compounds. In this study, we carried out benzoate fermentation using S. maritimus. In order to enhance benzoate productivity using cellulose as the carbon source, we constructed endo-glucanase secreting S. maritimus. RESULTS: After 4 days of cultivation using glucose, cellobiose, or starch as a carbon source, the maximal level of benzoate reached 257, 337, and 460 mg/l, respectively. S. maritimus expressed ß-glucosidase and high amylase-retaining activity compared to those of S. lividans and S. coelicolor. In addition, for effective benzoate production from cellulosic materials, we constructed endo-glucanase-secreting S. maritimus. This transformant efficiently degraded the phosphoric acid swollen cellulose (PASC) and then produced 125 mg/l benzoate. CONCLUSIONS: Wild-type S. maritimus produce benzoate via a plant-like ß-oxidation pathway and can assimilate various carbon sources for benzoate production. In order to encourage cellulose degradation and improve benzoate productivity from cellulose, we constructed endo-glucanase-secreting S. maritimus. Using this transformant, we also demonstrated the direct fermentation of benzoate from cellulose. To achieve further benzoate productivity, the L-phenylalanine availability needs to be improved in future.


Assuntos
Ácido Benzoico/metabolismo , Celulose/metabolismo , Amido/metabolismo , Streptomyces/metabolismo , Amilases/genética , Amilases/metabolismo , Fermentação , Oxirredução , Streptomyces/genética , beta-Glucosidase/genética , beta-Glucosidase/metabolismo
6.
Int J Biol Macromol ; 45(1): 33-41, 2009 Jul 01.
Artigo em Inglês | MEDLINE | ID: mdl-19447257

RESUMO

Effective application of elastin materials for vascular grafts in tissue engineering requires these materials to retain the elastic and biological properties of native elastin. To clarify the influence of soluble elastin isotypes on vascular smooth muscle cells (VSMCs), soluble elastin was prepared from insoluble elastin by hydrolysis with oxalic acid. Its fractions were separated and classified into three isotypes. Elastin retaining 2.25 mol% of cross-linked structures exhibited significant differentiation of VSMCs, which adhered to the elastin with contraction phenotypes similar to that of native elastin, causing proliferation to cease. This trend was more strongly demonstrated in cotton-like elastin fibers with a new cross-linker. The results suggest that elastin isotypes could be applied as new effective biomaterials for suppressing intimal hyperplasia in vascular grafts.


Assuntos
Diferenciação Celular/efeitos dos fármacos , Elastina/síntese química , Elastina/farmacologia , Hiperplasia/prevenção & controle , Engenharia Tecidual/métodos , Aminoácidos/análise , Animais , Bromodesoxiuridina , Cromatografia em Gel , Elastina/química , Citometria de Fluxo , Estrutura Molecular , Peso Molecular , Músculo Liso Vascular/efeitos dos fármacos , Espectrofotometria Ultravioleta , Sus scrofa
7.
Ann Thorac Surg ; 82(4): 1489-93, 2006 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-16996959

RESUMO

PURPOSE: A temporary epicardial pacing wire (TEPW) has been routinely placed in patients undergoing cardiac surgery. However, its fixation or removal occasionally causes troublesome complications. The aim of this study is to develop a novel TEPW using biodegradable film to fix the electrode to the epicardium without needle stabbing. DESCRIPTION: A biodegradable film was prepared with poly(L-lactide-co-epsilon-caprolactone). The film has a honeycomb-patterned structure that serves as a temporary adhesive for the myocardial surface, and the electrode was incorporated within the film. The novel TEPW was placed on the ventricular epicardium of dogs (group A, n = 5). As a control, conventional TEPW was inserted (group B, n = 6). The pacing threshold, R wave amplitude, impedance, and slew rate were measured at postoperative days 0, 1, 3, 5, 7, and 14, and complications after removal were checked. EVALUATION: All measurements in both groups were identified and differences were not observed. In addition, the novel TEPWs could be easily removed without related complications. CONCLUSIONS: This novel TEPW is safe and feasible for postoperative management of cardiac surgeries.


Assuntos
Implantes Absorvíveis , Arritmias Cardíacas/terapia , Marca-Passo Artificial , Adesivos Teciduais/uso terapêutico , Animais , Arritmias Cardíacas/etiologia , Caproatos/uso terapêutico , Estimulação Cardíaca Artificial , Procedimentos Cirúrgicos Cardíacos/efeitos adversos , Materiais Revestidos Biocompatíveis/uso terapêutico , Cães , Eletrodos Implantados , Desenho de Equipamento , Feminino , Lactonas/uso terapêutico , Masculino , Pericárdio , Poliésteres/uso terapêutico , Técnicas de Sutura
8.
Biomaterials ; 27(9): 1797-802, 2006 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-16293301

RESUMO

Honeycomb-patterned films have been reported to be useful for scaffolds of cell culture in tissue engineering. In the present study, we investigated a new compound, dioleoylphosphatidylethanolamine (DOPE), a naturally derived phospholipid having unsaturated fatty acid moieties, as a surfactant for fabricating honeycomb-patterned poly(d,l-lactide) (PLA) film. Only DOPE among commercially available phospholipids was useful as a surfactant, and it showed good solubility in PLA/chloroform solution and an excellent property for fabricating honeycomb-patterned film (the concentration of DOPE was from 0.2% to 20% by weight based on the weight of PLA). The pore size of the honeycomb was uniform, and all pores were interconnected with each other. The contact angle of water on the honeycomb-patterned film was affected by the amount of DOPE. Time-of-flight secondary ion mass spectrometer (TOF-SIMS) data suggested that DOPE was concentrated on the surface of the honeycomb-patterned film. To investigate cell proliferation and adhesion on the honeycomb-patterned film, NIH3T3 fibroblast cells were cultured on the film. The NIH3T3 cells adhered well on the honeycomb-patterned PLA film with DOPE (PLA-DOPE) and showed good cell proliferation compared to that on honeycomb-patterned PLA film fabricated with a copolymer (CAP) of dodecylacrylamide and omega-carboxyhexylacrylamide (PLA-CAP). These results suggest that the honeycomb-patterned PLA-DOPE can be applicable as a scaffold for cells with better profiles in comparison with PLA-CAP.


Assuntos
Materiais Biocompatíveis/química , Ácido Láctico/química , Fosfatidiletanolaminas/química , Polímeros/química , Tensoativos/química , Animais , Materiais Biocompatíveis/síntese química , Biodegradação Ambiental , Adesão Celular , Proliferação de Células , Espectrometria de Massas , Camundongos , Células NIH 3T3 , Poliésteres , Solubilidade , Propriedades de Superfície
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