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1.
Intern Med ; 62(18): 2743-2746, 2023.
Artigo em Inglês | MEDLINE | ID: mdl-37722924

RESUMO

We herein report a 90-year-old immunocompromised woman who developed right upper limb weakness and right ptosis with a miotic pupil 1 week after oral therapy for zoster on the right T2 dermatome. The right pupil was dilated with instillation of 1% apraclonidine, indicating Horner's syndrome. The patient was treated with intravenous acyclovir and methylprednisolone. Focal weakness related to zoster, generally known as segmental zoster paresis, improved over five months, but Horner's syndrome remained. We suggest that aggressive intravenous treatment should be considered for rare cases of zoster that occur with a combination of these two neurological conditions.


Assuntos
Blefaroptose , Herpes Zoster , Síndrome de Horner , Idoso de 80 Anos ou mais , Feminino , Humanos , Aciclovir/uso terapêutico , Herpes Zoster/complicações , Herpes Zoster/diagnóstico , Herpes Zoster/tratamento farmacológico , Síndrome de Horner/complicações , Síndrome de Horner/diagnóstico , Paresia/etiologia
2.
Mol Plant Pathol ; 10(3): 361-74, 2009 May.
Artigo em Inglês | MEDLINE | ID: mdl-19400839

RESUMO

In order to clone and analyse the avirulence gene AVR-Pia from Japanese field isolates of Magnaporthe oryzae, a mutant of the M. oryzae strain Ina168 was isolated. This mutant, which was named Ina168m95-1, gained virulence towards the rice cultivar Aichi-asahi, which contains the resistance gene Pia. A DNA fragment (named PM01) that was deleted in the mutant and that co-segregated with avirulence towards Aichi-asahi was isolated. Three cosmid clones that included the regions that flanked PM01 were isolated from a genomic DNA library. One of these clones (46F3) complemented the mutant phenotype, which indicated clearly that this clone contained the avirulence gene AVR-Pia. Clone 46F3 contained insertions of transposable elements. The 46F3 insert was divided into fragments I-VI, and these were cloned individually into a hygromycin-resistant vector for the transformation of the mutant Ina168m95-1. An inoculation assay of the transformants revealed that fragment V (3.5 kb) contained AVR-Pia. By deletion analysis of fragment V, AVR-Pia was localized to an 1199-bp DNA fragment, which included a 255-bp open reading frame with weak homology to a bacterial cytochrome-c-like protein. Restriction fragment length polymorphism analysis of this region revealed that this DNA sequence co-segregated with the AVR-Pia locus in a genetic map that was constructed using Chinese isolates.


Assuntos
Agricultura , Genes Fúngicos , Magnaporthe/genética , Magnaporthe/isolamento & purificação , Oryza/microbiologia , Pareamento de Bases/genética , Sequência de Bases , Segregação de Cromossomos , Clonagem Molecular , Sequência Conservada , Cosmídeos , Cruzamentos Genéticos , DNA Fúngico/genética , Teste de Complementação Genética , Japão , Magnaporthe/patogenicidade , Dados de Sequência Molecular , Fases de Leitura Aberta/genética , Fenótipo , Técnica de Amplificação ao Acaso de DNA Polimórfico , Deleção de Sequência , Transformação Genética , Virulência
3.
Biosci Biotechnol Biochem ; 73(4): 861-4, 2009 Apr 23.
Artigo em Inglês | MEDLINE | ID: mdl-19352027

RESUMO

Twenty-one strains of Amylomyces rouxii isolated from starters of Asian fermented foods were divided into two groups, lactic acid (LA) and fumaric and malic acid (FMA) producers, by organic acid productivity in liquid culture. Phylogenetic analysis based on the ldhB gene, ribosomal RNA encoding DNA (rDNA) internal transcribed spacer (ITS) sequence, and genome-wide amplified fragment length polymorphism (AFLP) revealed that A. rouxii was grouped into two major clusters as to organic acid accumulation, corresponding to Rhizopus oryzae and Rhizopus delemar. These observations suggest that the species A. rouxii is composed of two distinct types, derived from R. oryzae or R. delemar via domestication in the starters.


Assuntos
Rhizopus/classificação , Análise do Polimorfismo de Comprimento de Fragmentos Amplificados , DNA Fúngico/genética , DNA Ribossômico/genética , Etanol/metabolismo , L-Lactato Desidrogenase/genética , Filogenia , Rhizopus/genética , Rhizopus/metabolismo
4.
Fungal Genet Biol ; 45(12): 1543-51, 2008 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-18854221

RESUMO

In many eukaryotic organisms, the non-homologous end-joining (NHEJ) system is a major pathway for the repair of DNA double-strand breaks (DSBs). DNA ligase IV is a component of the NHEJ system and is strictly required for the NHEJ system in Saccharomyces cerevisiae and in Neurospora crassa. To investigate the functions of DNA Ligase IV in Magnaporthe grisea, we generated deletion mutants of MGLIG4, which encodes a homolog of N. crassa DNA Ligase IV. Mutants (mglig4) showed no defects in asexual or sexual growth, and were fully pathogenic. Compared to the wild-type, mglig4 exhibited weak sensitivity to a DNA-damaging agent, camptothecin. In addition, the frequency of targeted-gene replacement was relatively elevated in mglig4, although this varied in a gene-dependent manner. Surprisingly, non-homologous integration of DNA was frequently observed in mglig4 transformants. Our results demonstrate that MgLig4 is involved in, but not essential for, the NHEJ system in M. grisea.


Assuntos
DNA Ligases/metabolismo , Reparo do DNA , DNA Fúngico/metabolismo , Proteínas Fúngicas/metabolismo , Magnaporthe/enzimologia , Magnaporthe/fisiologia , Antifúngicos/farmacologia , Camptotecina/farmacologia , DNA Ligase Dependente de ATP , DNA Ligases/genética , Proteínas Fúngicas/genética , Deleção de Genes , Magnaporthe/efeitos dos fármacos , Magnaporthe/genética , Filogenia , Recombinação Genética , Homologia de Sequência
5.
Bioresour Technol ; 97(18): 2470-3, 2006 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-16324844

RESUMO

The objectives of this study were to evaluate the fundamental properties of potato pulp and to explore its potential uses. Lactic acid bacteria were the dominant microbes in potato pulp over the season (maximum being 10(8)/g). The water content in potato pulp was approximately 80% in all season. Starch and fiber were the main components of potato pulp, amounting to 80% of the dried matter regardless of the season. The fermentation of potato pulp by Rhizopus oryzae progressed only in the potato pulp extracted at the end of the season. This indicated that the fermentation of potato pulp was influenced by the dates of extraction, and it was assumed that the quality of the components, such as pectin, varied with such dates.


Assuntos
Rhizopus/metabolismo , Solanum tuberosum/microbiologia , Fermentação , Solanum tuberosum/metabolismo , Fatores de Tempo
6.
Curr Genet ; 42(6): 322-31, 2003 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-12612805

RESUMO

We investigated a DNA fragment and its flanking region deleted in the spontaneous Pi-a virulent mutant of Magnaporthe grisea Ina168. A new transposon-like sequence was identified from a region adjacent to the deleted fragment and was named Occan. Occan contained a 2,259-bp ORF interrupted by one 63-bp intron and had both a TA dinucleotide and 77 bp of perfect inverted repeats at both termini, without direct repeats. These features indicated that Occan is a member of the Fot1 family. RT-PCR analysis confirmed the expression of the putative transposase and the presence of an intron. Southern analysis of pulse-field gel electrophoresis-separated chromosomes indicated that Occan was dispersed in all chromosomes of the rice pathogen, Ina168. Copy numbers of Occan were also preserved in a host-specific manner amongst M. grisea isolates. In particular, rice pathogens contained a large number of the element inserted into their genome. Phylogenetic analysis with other known members of the Fot1 family revealed that Occan was dissimilar to any other known elements and it is thus proposed that Occan be separated to a new subfamily.


Assuntos
Elementos de DNA Transponíveis , Magnaporthe/genética , Sequência de Aminoácidos , Sequência de Bases , Dados de Sequência Molecular , Filogenia , Técnica de Amplificação ao Acaso de DNA Polimórfico , Análise de Sequência de DNA , Homologia de Sequência , Transposases/genética
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