[The use of biochemical markers in diagnosis of endometriosis].

The aim of this study was to evaluate Ca 125 II, C-reactive protein (CRP) and serum amyloid A (SAA) and anticardiolipin antibody (aCL) concentrations for the diagnosis of pelvic endometriosis. The study population consisted of 15 women without endometriosis, as confirmed by laparoscopy (group A), and 35 patients with pelvic endometriosis. All of them were diagnosed by laparoscopy or laparotomy. Group B patients were divided into those at stages I and II of the disease (B I/II) and those at stages III and IV (B III/IV). Blood samples were obtained twice during the menstrual cycle: on day 1-3 of the cycle and on day 8-10 of the cycle. Our results claim that the measurement of these molecules may therefore provide a valuable tool in the diagnosis and management of endometriosis.

[Immunocompetent cells in pathogenesis of endometriosis].

The pathogenic mechanisms which lead to development of endometriosis are still unclear. Despite that the immunological mechanisms are not the primary, there is no doubt that they take a part in pathogenesis of the disease. In current review, the influence of the cells of the immune system which are connected with endometriosis is described.

[Changes of activation in T-lymphocyte subpopulations in peripheral blood during pregnancy].

From an immunological point of view during pregnancy the interactions between the steroid sex hormones and immunocompetent cells as a part of the dynamic local and peripheral immune response is of a particular interest. The aim of our study is to investigate the expression of the early activation marker CD69 in T-lymphocytes subpopulation: CD4+ and CD8+ in peripheral blood from pregnant and non-pregnant women. Our data clearly demonstrate an increase of the percentage of activated CD4+ lymphocytes in pregnant women in comparison to non-pregnant and this difference is statistically significant. A similar but not statistically significant dependency is observed in CD8+ and CD69+ lymphocytes. From the obtained results we conclude that during pregnancy the activation of CD4+ lymphocytes is increased, which probably leads to an increased production of cytokines that shifts the immune response to Th2 type which is protective for pregnancy. This could be partly due to the increased levels of progesterone.

[Cell proliferation in endometrium of women with endometriosis]. / Kletuchna proliferatsiia v endometriuma na zheni s endometrioza.

The purpose of this study was to describe the endothelial cell activity of the endometrial tissue in women with and without endometriosis, and find out the diagnostic opportunities for everyday gynecologic practice. There were 30 women with laparoscopic proved endometriosis involved in our study and 27 women with no endometriosis used as a control group. The histological findings were scored after Noyes criteria and classified according to the phase of the menstrual cycle. We found out a significant difference between the two groups, especially during the proliferate phase of the menstrual cycle.