RESUMO
Xanthohumol is a major prenylated, hydrophobic flavonoid found in the female inflorescences of the hop plant (Humulus lupulus L.). In this study, we examined the effects of xanthohumol-rich hop extract containing 17.8% xanthohumol and 12.4% isoxanthohumol on the differentiation and adipogenesis of 3T3L1 cells. We observed that the extract inhibited the differentiation of 3T3L1 cells and intracellular fat droplets via the regulation of adipogenic factors such as the peroxisome proliferator-activated receptor gamma, CCAAT/enhancer binding protein alpha, and adipocyte fatty acid-binding protein.
Assuntos
Adipócitos/citologia , Adipogenia/efeitos dos fármacos , Diferenciação Celular/efeitos dos fármacos , Fibroblastos/citologia , Flavonoides/farmacologia , Humulus/química , Extratos Vegetais/farmacologia , Propiofenonas/farmacologia , Adipócitos/metabolismo , Adipogenia/genética , Animais , Fármacos Antiobesidade , Proteína alfa Estimuladora de Ligação a CCAAT/metabolismo , Linhagem Celular , Proteínas de Ligação a Ácido Graxo/metabolismo , Flavonoides/isolamento & purificação , Metabolismo dos Lipídeos/efeitos dos fármacos , Metabolismo dos Lipídeos/genética , Camundongos , PPAR gama/metabolismo , Extratos Vegetais/isolamento & purificação , Propiofenonas/isolamento & purificação , Xantonas/isolamento & purificação , Xantonas/farmacologiaRESUMO
Xanthohumol is the major prenylated flavonoid of female inflorescences of the hop plant (Humulus lupulus L.) and is a hydrophobic flavonoid. We examined the effects of dietary xanthohumol-rich hop extract in obese rats that was induced by feeding a high-fat diet. Dietary xanthohumol-rich hop extract significantly lowered the body weight gain of these rats compared to rats fed a high-fat diet without the extract. The increase of body weight, liver weight, and triacylglycerol levels in the plasma and liver of the rats fed a high-fat diet was ameliorated by dietary xanthohumol-rich hop extract. Dietary xanthohumol-rich hop extract tended to reduce hepatic fatty acid synthesis through the reduction of hepatic SREBP1c mRNA expression in the rats fed a high-fat diet. The excreted of triacylglycerol into feces also was promoted by dietary xanthohumol-rich hop extract. Plasma adiponectin levels in the rats fed a high-fat diet also tended to be elevated by dietary xanthohumol-rich hop extract. Thus, xanthohumol-rich hop extract may inhibit the increase of body weight, liver weight, and triacylglycerol in the plasma and liver induced by feeding high-fat diet through the regulation of hepatic fatty acid metabolism and inhibition of intestinal fat absorption. Therefore, xanthohumol-rich hop extract may exert preventive function on the increase of body weight and tissue triacylglycerol levels by overnutrition.
