Psychrophilic one- and two-step systems for pre-treatment of winery waste water.

The operation performance of a single and two (in series) laboratory UASB reactors (working volume of 2.7 l, recycle ratio varied from 1:1 to 1:18) treating diluted wine vinasse was investigated under psychrophilic conditions (4-10 degrees C). For a single UASB reactor seeded with granular sludge, the average organic loading rates (OLR) applied were 4.7, 3.7 and 1.7 g COD/l/d (hydraulic retention times (HRTs) were around 1 d) at 9-11, 6-7 and 4-5 degrees C, respectively. The average total COD removal for preacidified vinasse wastewater was around 60% for all the temperature regimes tested. For two UASB reactors in series, the average total COD removal for treatment of non-preacidified wastewater exceeded 70% (the average OLRs for a whole system were 2.2, 1.8 and 1.3 g COD/l/d under HRTs of 2 days at 10, 7 and 4 degrees C, respectively). In situ determinations of kinetic sludge characteristics (Vm and Km) revealed the existence of substantial mass-transfer limitations for the soluble substrates inside the reactor sludge bed. Therefore an application of higher recycle rations is essential for enhancement of UASB pre-treatment under psychrophilic conditions. The produced anaerobic effluents were shown to be efficiently post-treated aerobically--final effluent COD concentrations were around 0.1 g/l.

One- and two-stage upflow anaerobic sludge-bed reactor pretreatment of winery wastewater at 4-10 degreesC.

The operating performance of a single and two (in series) laboratory upflow anaerobic sludge-bed (UASB) reactors (2.7-L working volume, recycle ratio varied from 1:1 to 1:18) treating diluted wine vinasse was investigated under psychrophilic conditions (4-10 degreesC). For a single UASB reactor seeded with granular sludge, the average organic loading rates (OLRs) applied were 4.7, 3.7, and 1.7 g of chemical oxygen demand (COD)/(L.d) (hydraulic retention times [HRTs] were about 1 d) at 9-11, 6 to 7, and 4 to 5 degreesC, respectively. The average total COD removal for preacidified vinasse wastewater was about 60% for all the temperature regimes tested. For two UASB reactors in series, the average total COD removal for treatment of non-preacidified wastewater exceeded 70% (the average OLRs for a whole system were 2.2, 1.8, and 1.3 g of COD/[L.d] under HRTs of 2 d at 10, 7, and 4 degreesC, respectively). In situ determinations of kinetic sludge characteristics (apparent Vm and Km) revealed the existence of substantial mass transfer limitations for the soluble substrates inside the reactor sludge bed. Therefore, application of higher recycle ratios is essential for enhancement of UASB pretreatment under psychrophilic conditions. The produced anaerobic effluents were shown to be efficiently posttreated aerobically: final effluent COD concentrations were about 0.1 g/L. Successful operation of the UASB reactors at quite low temperatures (4-10 degreesC) opens some perspectives for application of high-rate anaerobic pretreatment at ambient temperatures.