[Synergism and interference in the combined biological action of ionizing and SHF radiation]. / Sinergizm i interferentsiia pri sochetannom biologicheskom deistvii ioniziruiushchikh i SVCh-izluchenii.

In experiments with 933 (CBA x C57Bl)F1 mice it has been found that with any variant of alternation and sequence of single ionizing and SHF irradiation with lethal doses, the radiation affection caused by gamma quanta is aggravated. Preirradiation with gamma or SHF radiation ameliorates the severity of the affection caused by the subsequent SHF radiation. Parenteral injection of colloidal sulfur 60 min before SHF radiation causes a 2-5-fold increase in the survival rate of exposed and protected mice as compared to unprotected animals.

[Purified sulfur as an agent to relieve the side effects in the radiation therapy of cervical cancer]. / Ochishchennaia sera kak sredstvo oslableniia pobochnykh reaktsii pri luchevoi terapii raka sheiki matki.

The theoretical basis of this work is the development of autosensitization in exposure to ionizing radiation and well-known desensitizing action of sulphuric agents. To reduce clinical manifestations of a reaction to combined radiotherapy 34 women with diagnosis of cervical cancer (stages I and II) were given 0.5-1 g of purified sulphur mixed with 0.25 g of glucose in the morning every 2-3 hours before irradiation, per os; 24 patients received placebo (a mixture of egg powder with glucose), in 21 patients no protective means were used. All 79 patients were given unified adjuvant therapy (polyvitamins, leukogens, folic acid) and diet No 15. A significant decrease in the reaction to therapeutic irradiation was noted in the study group. No side-effects were observed.

[The immuno-microbiological status of mice after partial exposure to x-rays and the possibility of its correction using immunoglobulin]. / Immunomikrobiologicheskii status myshei pri partsial'nom rentgenovskom obluchenii i vozmozhnost' ego korrektsii s pomoshch'iu immunoglobulina.

Partial X-irradiation of the hind part of mouse body with a dose of 12 Gy (LD15/30) was shown to produce a pronounced harmful effect on the clinico-hematologic and immuno-microbiological characteristics that could be coped with by a single subcutaneous injection of homologous immunoglobulin (200 mg/kg) 2 h following irradiation.

[Sorption of bacteria by blood cells as an indicator of the reaction of animals to the action of different doses of gamma rays]. / Sorbtsiia bakterii kletkami krovi kak pokazatel' reaktsii zhivotnykh na gamma-vozdeistvie v raznykh dozakh.

In experiments with mice, guinea pigs, and dogs, a study was made of the sorptive capacity of peripheral blood cells after different gamma-radiation doses with reference to living Escherichia coli cells. The sorptive capacity of blood cells was inhibited in exposed animals the inhibition being maximum during the first 3 days following irradiation. Homologous immunoglobulin administered to mice 24 h before irradiation prevented the diminution of the sorptive capacity of cells and stimulated it during the first week following irradiation.

[Methods of enhancing the radioprotective efficacy of serum immunoglobulin]. / Nekotorye sposoby povysheniia protivoluchevoi éffektivnosti immunoglobulina krovi.

In this work the authors propose three ways of increasing the radioprotective efficiency of immunoglobulin: in vitro irradiation with gamma-quanta (60Co, 20,000 Gy), increasing the time of storage of whole blood (raw material for immunoglobulin production) up to 5-7 days at +4 degrees C, and the application of immunoglobulin with antibiotics. In experiments in vitro it was first shown that a combination of immunoglobulin with gentamicin exerted a pronounced bactericidal effect on E. coli. The effect was absent with immunoglobulin and markedly less pronounced with gentamicin used separately.

[Effect of homologous immunoglobulin with normal anti-tissue antibodies on the development of strontium-90-induced osteosarcomas in rats]. / Vliianie gomologichnogo immunoglobulina s normal'nymi protivotkanevymi antitelami na razvitie u krys osteosarkom, indutsirovannykh strontsiem-90.

In experiments on Wistar rats it was shown that homologous immunoglobulin (a single dose of 25 mg/kg), with normal antitissue antibodies, subcutaneously injected in the following manner: a single injection 90 days or 6 months, or double injection 3 and 6 months after intraperitoneal administration of strontium 90 (11.1 kBq/kg) reduces osteosarcoma occurrence and increases the average lifetime of animals.

[New data on the biological action of normal immunoglobulins]. / Novye dannye o biologicheskom deistvii normal'nykh immunoglobulinov.

Experiments on 2,520 CBA mice (CBA X X C57BL) F1 nice have shown that the injection of homologous serum immunoglobulins (obtained from intact and blood-stimulated animals), made 2 hours after gamma irradiation from a 60Co source, prevents the development of intestinal dysbacteriosis and endogenous infection. The injection of mouse and human immunoglobulins to nonirradiated mice improved their resistance to experimental infection with Escherichia coli live culture, increased the expression of receptors to the Fc-fragments of IgG in peritoneal macrophages and enhanced the physical working capacity of the animals. The preparations containing normal antitissular antibodies have proved to be particularly effective. In mice, rabbits and dogs the preparations under test have produced no changes in the general state of the animals, no local reactions and no disturbances in the cardiovascular activity.

[Effect of homologous serum globulin preparations on the intestinal automicroflora of mice after irradiation]. / Issledovanie vliianiia preparatov gomologichnykh syvorotochnykh globulinov na automikrofloru kishechnika myshei posle oblucheniia.

In experiments on 4000 noninbred and CBA mice the influence of the injections of alpha-, beta- and gamma-globusin, IgG and IgM, obtained from the sera of hemostimulated and intact mice, on the intestinal microflora after irradiation has been studied. The experiments have revealed that 3 subcutaneous or intraperitoneal injections of 1 mg per mouse, made 2, 24 and 48 hours after the irradiation of the animals with gamma-rays in a dose of 700 r, considerably reduce the intensity of the accumulation of opportunistic bacteria in the small and large intestines, commonly occurring in irradiated animals. A decrease in the number of lactobacteria is less pronounced. The preparations of globulin and IgG obtained from hemostimulated mice, i.e. enriched with normal tissue antibodies, have proved to be most effective.

[Comparative radioprotective efficacy of native and irradiated in vitro immunoglobulins from horse serum]. / Sravnitel'naia protivoluchevaia éffektivnost' nativnogo i obluchennogo in vitro immunoglobulinov syvorotki krovi loshadei.

The therapeutic application of native and in vitro exposed (20000 Gy immunoglobulins of horse blood serum increased the survival of irradiated (LD75-95) animals, normalized the quantitative and qualitative status of the small intestine microflora and prevented enterobacteria from penetrating the internal organs. The irradiated preparations were more active than native ones.

[Further study of the nature and characteristics of the rosette formation phenomenon by a modified Jerne's method]. / Dal'neishee izuchenie prirody i osobennostei iavleniia bliashkoobrazovaniia pri modifitsirovannom metode Erne.

Experiments were conducted on 105 mongrel female albino mice weighing 18--22 g (intact, irradiated in a dose of 700 r with gamma-rays 60Co or immunized with SRBC) and on intact hybrids F1, CBAxC57BL. Phase contract microscopy showed autoimmune hemolysis plaques to be large or irregular and to posses a girdle made up of deformed and lysed erythrocytes and to contain stroma "ghosts" of the latter around the central cell. Plaques obtained in hypertensive medium were always round and the bordering girdle consisted of intact erythrocytes, whereas the plaque filed was homogeneous or contained granules. The concentration of sodium, potassium, calcium, and magnesium atoms in the suspension fluid of the preparations failed to exceed their content in the cells (as demonstrated by Jerne's method modified by the authors on a mixture of spleen cells with autologous blood). Addition of antiglobulin serum into the preparation on a slide in a 1:5 dilution suppressed the formation of autoimmune plaques, and in a dilution of 1:50--increased it considerably.