1.
Chemistry
; 23(47): 11230-11233, 2017 Aug 22.
Artigo
em Inglês
| MEDLINE
| ID: mdl-28688127
RESUMO
An efficient synthesis of the molybdopterin/molybdenum cofactor (Moco) oxidation product dephospho Formâ A is described that assembles the pteridinone system starting from an iodinated aminopyrazine. The sodium salt of dephospho Formâ A could be purified by precipitation from methanol, which paved the way to the title compound in the 100â mg range. By HPLC, the synthetic material was compared with a sample isolated from a recombinant Moco containing protein. Analysis of dephospho Formâ A is the only method that allows the quantification of the Moco content of crude cell extracts and recombinant protein preparations.