[Urogenital Injury in Polytrauma Patients: a Five-year Epidemiological Study]. / Urogenitální poranení u pacientu u polytraumatu: petiletá epidemiologická studie.

INTRODUCTION The authors present an overview of polytrauma patients with urinary tract injuries managed in the Level I trauma centre. The purpose of this study was to evaluate the frequency of injuries to the urinary system, the mechanism of their injury and the method of treatment in the cohort of polytrauma patients treated in the trauma centre. MATERIAL AND METHODS Between 2012 and 2016, a total of 231 polytrauma patients were treated, of whom 47 (20.3%) also suffered a urotrauma. The renal trauma, ureteral trauma, bladder trauma and urethral trauma were classified according to the American Association for the Surgery of Trauma (AAST) scoring scale. RESULTS The non-operative treatment was opted for in 24 patients (86%) with Grade I and II kidney injury, whereas a surgical revision was indicated in line with the European Association of Urology (EAU) Guidelines in 9 patients (100%) with Grade III renal trauma or higher. The bladder trauma was treated non-operatively in two patients (22.2%) with Grade I and II bladder injury and operatively in 7 patients (77.7%) with Grade III injury or higher. After the operative management of urogenital injuries in 18 patients (38%), complications occurred in 7 patients (39%). A total of 7 patients (14.9%) died as a consequence of polytrauma with urinary tract injuries and in 5 of them the death occurred within 30 days of polytrauma, but no urinary tract injury led directly to death of the patient. DISCUSSION Kidneys are the most frequently injured uropoietic organ. A civilian kidney trauma is present in up to 5% of trauma patients and accounts for 24% of abdominal injuries. The computed tomography scanning with intravenous contrast (CT IVU) remains the Gold Standard. In recent decades there was a shift in treating the prevailing majority of patients with a kidney trauma, namely from surgical revision to non-operative treatment. This trend was also observed by the authors in their own cohort. CONCLUSIONS Based on the evaluation of their own cohort of patients, the authors state that the diagnostic algorithm including CT IVU and also UCG in indicated patients are adequate to timely diagnose the urinary tract injuries, including polytraumas. The study confirmed that the most frequent injury to the urinary tract in polytrauma patients is the kidney trauma and that the urinary tract injury is not usually the cause of death in polytraumatized patients. Key words: polytrauma, urotrauma, urogenital organ injury, kidney, urinary bladder, AAST.

Rapid screening of depression and anxiety in cancer patients: Interview validation of emotion thermometers.

BACKGROUND AND OBJECTIVE: This study aimed to determine the degree of depression and anxiety in cancer patients using the Emotion Thermometers (ET) and confirming their clinical usefulness compared to the gold standard interview, as well as determining optimal cut-off values for the appropriate identification of cancer patients' distress. METHODS: We included 238 cancer patients and we used ET (Emotion Thermometers) to screen depression and anxiety and the Beck depression inventory for adults (BDI-II), the Generalized Anxiety Disorder 7-item scale (GAD-7) and the Mini-International Neuropsychiatric Interview (M.I.N.I) was used as the criterial validity standard. RESULTS: The prevalence of anxiety on the M.I.N.I. was 24% and depression was 11%. The optimal value for diagnosis of depression from ET (Dep ET) appears to be > 4.5 (AUC 0.928) against M.I.N.I. Optimal score for anxiety from ET (AnxT ET) compared to GAD according to M.I.N.I. we determined the value of 3.5 (AUC 0.899). To determine the cut off score for distress using from ET (DT), we compared against GAD-7 and BDI-II RS (raw total score) and the most optimal was 4.5 (AUC 0.953). For analysis of the cut off score for quality of life (QoL) against the total sums of all parts of the ET, the value of 14.5 (AUC 0.892) forms the cut off between the negative and the positive clinical finding. CONCLUSIONS: The results of the study support the use of ET as a rapid screening tool for the detection of depression, anxiety and distress in cancer patients.

[The role of contrast-enhanced pulse inversion harmonic imaging (CEUS) and contrast-enhanced computed tomography (CECT) in the preoperative diagnosis of renal lesions]. / Die Rolle von kontrastmittelverstärktem "pulse inversion harmonic imaging" (CEUS) und kontrastmittelverstärkter Computertomographie (CECT) bei der präoperativen Diagnose von renalen Raumforderungen.

OBJECTIVE: The preoperative assessment of structural and functional changes in renal tumors using contrast-enhanced pulse inversion harmonic imaging (CEUS) and contrast-enhanced computed tomography (CECT). MATERIALS AND METHODS: All consecutive patients referred to two tertiary hospitals for surgery on suspicion of a malignant renal lesion, who had been examined under the predefined study protocol using CEUS and CECT, were prospectively included in the study. All renal lesions suspected of being malignant were subjected to histopathological examination. Lesions expected to be benign were followed up according to the study protocol. The accuracy of CEUS and CECT with the final histology or follow-up results and the statistically significant difference between the two imaging techniques was calculated. RESULTS: Over a period of 3 years (2008-2011), 68 of 93 patients examined met the study criteria. The prevalence of malignant tumors in the study was 72%. Fifty four (79%) patients underwent surgery and had a histologically confirmed renal tumor (clear cell carcinoma 45, urothelial papillocarcinoma 4, angiomyolipoma 1, oncytoma 3, xanthogranulomatous pyelonephritis 1) and 14 (21%) patients underwent regular follow-up. Specificity, sensitivity and area under the curve (AUC) reached 57.9%, 98% and 0.779 for CEUS and 52.6%, 98% and 0.753 for CECT. CONCLUSION: The results show that both imaging methods can reliably rule out malignant disease due to absence of enhancement. Taking into consideration that CEUS can be carried out without severe risk or discomfort, it is time to reconsider CEUS as the method of choice for diagnosis, while CECT should be reserved for staging.

[Two synchronous malignancies in a single nephrectomy specimen]. / Zwei synchrone Malignome in einem Nephrektomiepräparat.

We report a case of a 65-year-old man examined for hematuria and neurologic symptoms. Radiologically a tumour of the kidney was suspected, with metastatic involvement of the brain and both lungs. The urologists then performed a cytoreductive nephrectomy.In histopathological examination of the nephrectomy specimen a clear cell renal cell carcinoma (clear cell RCC) was found. However, an additional focus of a high-grade adenocarcinoma was discovered that turned out to be a metastasis of a poor differentiated lung adenocarcinoma. This diagnosis was supported by positive TTF1 and napsin-A as well as good clinical correlations.The article summarizes the differential diagnostic considerations of poor differentiated adenocarcinoma of the kidney, aimed at gross and microscopic morphology, immunohistochemistry and clinical-pathological correlation. A review of literature about a tumour-in-tumour metastasis is included.

Wilms tumor gene 1 (WT1), TP53, RAS/BRAF and KIT aberrations in testicular germ cell tumors.

PURPOSE: Wilms tumor gene 1 (WT1), a zinc-finger transcription factor essential for testis development and function, along with other genes, was investigated for their role in the pathogenesis of testicular germ cell tumors (TGCT). METHODS: In total, 284 TGCT and 100 control samples were investigated, including qPCR for WT1 expression and BRAF mutation, p53 immunohistochemistry detection, and massively parallel amplicon sequencing. RESULTS: WT1 was significantly (p < 0.0001) under-expressed in TGCT, with an increased ratio of exon 5-lacking isoforms, reaching low levels in chemo-naïve relapsed TGCT patients vs. high levels in chemotherapy-pretreated relapsed patients. BRAF V600E mutation was identified in 1% of patients only. p53 protein was lowly expressed in TGCT metastases compared to the matched primary tumors. Of 9 selected TGCT-linked genes, RAS/BRAF and WT1 mutations were frequent while significant TP53 and KIT variants were not detected (p = 0.0003). CONCLUSIONS: WT1 has been identified as a novel factor involved in TGCT pathogenesis, with a potential prognostic impact. Distinct biologic nature of the two types of relapses occurring in TGCT has been demonstrated. Differential mutation rate of the key TGCT-related genes has been documented.

[Carcinoma of the penis]. / Karcinom penisu.

BACKGROUND: Experience with diagnosis and results of the treatment cancer of the penis are comparatively sparse. We present our experience and treatment results of patients with this diagnosis. METHODS AND RESULTS: The group of 286 patients were diagnosed and treated between 1981-2006 at the First Medical School, Charles University, Prague, Department of Urology, District Hospital Ustí nad Labem. and University Hospital Na Bulovce, Department of Urology, Prague. Average age of the patients was 55 year. In 93% of cases the disease originated from an untreated phimosis Squamous cell cancer was found in 91% of the biopsies. Organ preserving surgery was done in 108 patients. Interstitial brachytherapy Ir 192 was first used as organ preserving treatment modality for patients with SCC in Czech Republic. 106 inguinal lymphadenectomies were performed, in 41 cases in a modified form. For the patients with positive lymph nodes, chemotherapy as neoadjuvant or adjuvant treatment approach was indicated. Actinotherapy was indicated minimally. Treatment complications intra- or postoperative were minimal. From our cohort 79.7% patients survived, 39.8% of them survived more than 10 years. CONCLUSIONS: We recommended concentrating patients with this relatively rare disease to the special department with complete diagnostic and treatment care.