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Int Immunopharmacol ; 2(4): 453-61, 2002 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-11962725

RESUMO

A chimeric macaque/human (PRIMATIZED) anti-CD23 antibody, p6G5G1, demonstrated a strong inhibitory effect on IL-4 and anti-CD40 antibody-stimulated IgE production by human peripheral blood mononuclear cells (PBMCs). RNA analysis by both reverse transcription-polymerase chain reaction (RT-PCR) and Northern blot showed that p6G5G1 inhibited germline Cepsilon RNA synthesis, but had no effect on CD23 mRNA levels. These data suggest that p6G5G1 may inhibit immunoglobulin class switching to IgE through the inhibition of germline Cepsilon RNA synthesis. Early addition of p6G5G1 after stimulation by IL-4 and anti-CD40 was critical for IgE inhibition. In contrast, later addition of p6G5G1 still showed inhibition of increased levels of surface CD23, which is normally upregulated by stimulation with IL-4 and anti-CD40.


Assuntos
Anticorpos Monoclonais/imunologia , Anticorpos Monoclonais/farmacologia , Linfócitos B/efeitos dos fármacos , Regiões Constantes de Imunoglobulina/genética , Imunoglobulina E/genética , Receptores de IgE/imunologia , Transcrição Gênica/efeitos dos fármacos , Animais , Linfócitos B/metabolismo , Citometria de Fluxo , Humanos , Switching de Imunoglobulina/efeitos dos fármacos , Imunoglobulina E/biossíntese , Macaca , RNA Mensageiro/genética , RNA Mensageiro/metabolismo , Receptores de IgE/análise , Receptores de IgE/biossíntese , Receptores de IgE/genética , Reação em Cadeia da Polimerase Via Transcriptase Reversa , Fatores de Tempo
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