1.
J Microbiol Methods
; 60(2): 269-73, 2005 Feb.
Artigo
em Inglês
| MEDLINE
| ID: mdl-15590101
RESUMO
A real-time PCR assay is described for detection of the macrolide efflux gene, mef. Following amplification, unambiguous discrimination between the two mef subclasses, mef(A) and mef(E), is easily established using a melting curve analysis. The results of this novel assay were 100% concordant with a conventional PCR-RFLP approach but requires far less hands-on time. Furthermore, the real-time format offers semiquantitative results allowing identification of contaminated cultures and/or DNA preparations.