The Variation of Selected Physiological Parameters in Elm Leaves (Ulmus glabra Huds.) Infested by Gall Inducing Aphids.

Three aphid species, Eriosoma ulmi (L.), Colopha compressa (Koch) and Tetraneura ulmi (L.) induce distinct gall morphotypes on Ulmus glabra Huds.; opened and closed galls. Because the trophic relationship of aphids and their galls shows that throughout the gall formation aphids can elicit multiple physiological regulations, we evaluated the changes of hydrogen peroxide content (H2O2), cytoplasmic membrane condition, expressed as electrolyte leakage (EL) and concentration of thiobarbituric acid reactive substances (TBARS), as well as, the activity of catalase (CAT), guaiacol peroxidase (GPX) and ascorbate peroxidase (APX) in gall tissues, as well as, in damaged and undamaged parts of galled leaves. All aphid species increased EL from gall tissues and significantly upregulated APX activity in galls and galled leaves. Alterations in H2O2 and TBARS concentrations, as well as GPX and CAT activities, were aphid- and tissue-dependent. The development of pseudo- and closed galls on elm leaves did not have a clear effect on the direction and intensity of the host plant's physiological response. The different modes of changes in H2O2, TBARS, CAT and GPX were found in true galls of C. compressa and T. ulmi. Generally, physiological alterations in new plant tissues were quite different compared to other tissues and could be considered beneficial to galling aphids.

Expression of Thioredoxin/Thioredoxin Reductase System Genes in Aphid-Challenged Maize Seedlings.

Thioredoxins (Trxs) and thioredoxin reductases (TrxRs) encompass a highly complex network involved in sustaining thiol-based redox homeostasis in plant tissues. The purpose of the study was to gain a new insight into transcriptional reprogramming of the several genes involved in functioning of Trx/TrxR system in maize (Zea mays L.) seedlings, exposed to the bird cherry-oat aphid (Rhopalosiphum padi L.) or the rose-grass aphid (Metopolophium dirhodum Walk.) infestation. The biotests were performed on two maize genotypes (susceptible Zlota Karlowa and relatively resistant Waza). The application of real-time qRT-PCR technique allowed to identify a molecular mechanism triggered in more resistant maize plants, linked to upregulation of thioredoxins-encoding genes (Trx-f, Trx-h, Trx-m, Trx-x) and thioredoxin reductase genes (Ftr1, Trxr2). Significant enhancement of TrxR activity in aphid-infested Waza seedlings was also demonstrated. Furthermore, we used an electrical penetration graph (EPG) recordings of M. dirhodum stylet activities in seedlings of the two studied maize varieties. Duration of phloem phase (E1 and E2 models) of rose-grass aphids was about three times longer while feeding in Waza plants, compared to Zlota Karlowa cv. The role of activation of Trx/TrxR system in maintaining redox balance and counteracting oxidative-induced damages of macromolecules in aphid-stressed maize plants is discussed.

Poplar Tree Response to Feeding by the Petiole Gall Aphid Pemphigus spyrothecae Pass.

Pemphigus spyrothecae Pass. which is a member of the subfamily Pemphiginae is one of the gall-inducing aphids that occurs on poplar trees. Phloem feeding of a founding mother on leaf petiole results in the formation of a new organ, i.e., the spiral gall. This study documents aphid development inside the galls during the growing season and the effect of their feeding on leaf architecture and physiology of the host plant. In particular, leaf length, width, and area were measured, as well as hydrogen peroxide (H2O2) content, electrolyte leakage (EL), malondialdehyde (MDA) concentration, and the activity of ascorbate (APX) and guaiacol peroxidase (GPX) were determined in galls and galled leaves. The presence of petiole galls significantly decreased the length, width, and leaf area. Aphid activity increased H2O2 concentration in galls and EL from galls and leaf tissues, which was accompanied by a strong decrease in MDA content and both peroxidase activities, especially in gall tissues. It can be suggested that P. spyrothecae can manipulate physiological machinery of the host plant for its own benefit.

Tachycardia: The hidden cardiovascular risk factor in uncomplicated arterial hypertension.

Early detection and management of elevated blood pressure is crucial in reducing the burden of cardiovascular disease (CVD). The importance of an absolute risk assessment and patient risk stratification has been highlighted in the European hypertension guidelines since 2003. Amongst numerous risk factors influencing patient prognosis, elevated heart rate (HR) has been indicated as important predictor of future risk of hypertension, coronary heart disease, sudden cardiac death, heart failure, CVD, stroke, total cancer and mortality. Given that resting HR can be easily determined in clinical practice and modified by lifestyle changes as well as beta-blocker therapy, it seems reasonable that lowering resting HR should be a potential target to reduce disease burden and premature mortality. However, there is a lack of outcome studies of HR lowering in tachycardia-related hypertension. This review outlines the underlying mechanisms of early course hypertension pathophysiology with the critical role of the sympathetic nervous system activation, the prognostic significance of fast HR and the mechanistic rationale for the use of non-pharmacological approaches and/or highly long-acting cardioselective beta-blockers with some consideration given to betaxolol properties.

In vitro generation of pancreatic ß-cells for diabetes treatment. I. ß-like cells derived from human pluripotent stem cells.

Diabetes mellitus is a chronic disease that affects hundreds of millions of people worldwide. Type 1 diabetes (T1D) is characterized by the lack of pancreatic ß-cells that had been destroyed as a result of an autoimmune response. Therefore, in patients with T1D, the replacement therapy with functional ß-cells derived from extrinsic sources could be a preferable option as compared to insulin treatment. Unfortunately, successful transplantation of whole pancreata or pancreatic islets into patients with diabetes is available only to a fraction of them due to the scarcity of donors. The rapid development of cell reprogramming methods made it possible to generate large numbers of human ß-like cells derived from human embryonic stem cells (hESCs) or human induced pluripotent stem cells (hiPSCs). This review describes the basis of in vitro differentiaton protocols of ß-like cells that mimic changes of the main signaling pathways during the key stages of human and murine pancreas development, which are described first. During the last 15 years it was found that there are no important differences between hESCs and hiPSCs in their differentiation capacities into ß-like cells and the expression profiles of the key transcription factors. The in vitro produced ß-like cells are immature as demonstrated by functional tests in rodents and single-cell transcriptomic and proteomic analyses. After the transplantation of the ß cell progenitors into immunocompromised diabetic mice, a few weeks have to pass before the increased insulin levels in response to glucose load appear. There is a continuous progress in the development of open-type encapsulation devices which allow the vascularization of the transplanted cells and protect them against host's immune cells. The results of the first clinical trial of human partially differentiated endocrine progenitors of ß cells transplanted into patients with T1D will be published in the year 2019. It is hoped that further improvements in the techniques of large-scale generation of the ß-like cells derived from human pluripotent stem cells will bring us closer to their clinical application as a form of cause-directed therapy for people with diabetes.

Physiological Response of Basil Plants to Twospotted Spider Mite (Acari: Tetranychidae) Infestation.

The induction of plant resistance against pests is considered a potential method of controlling mite infestation as it restricts the use of chemical pesticides in herbal crops. Our goal was to investigate whether plant physiological response to mite feeding varied depending on basil cultivar and/or duration of mite infestation. The effect of plant acceptance, mite mortality rate, and changes in physiological parameters: malondialdehyde content (MDA), hydrogen peroxide (H2O2) concentration, and antioxidant enzyme activities, including guaiacol peroxidase (GPX) and catalase (CAT) were examined in this study. Tetranychus urticae Koch (Acari: Tetranychidae) infestation induced oxidative stress in three Ocimum basilicum L. cultivars: 'Sweet basil,' 'Purpurascens,' and 'Fino Verde.' The analysis of mite behavior and alteration in metabolic plant profiles showed different sensitivities of basil cultivars to biotic stress that were dependent on the cultivar and duration of infestation. All basil plants were suitable as host plants for T. urticae, but they varied in the level of susceptibility to mite feeding. O. basilicum 'Fino Verde' was the most suitable host for the twospotted spider mite. In turn, O. basilicum 'Purpurascens' was characterized by the lowest level of susceptibility to T. urticae feeding. The lowest acceptance, the highest mortality of twospotted spider mite individuals as well as decreased levels of H2O2 and MDA, significantly increased GPX activity and low level of CAT activity were recorded in O. basilicum 'Purpurascens' leaves. Research on plant responses to biotic stress can inform breeding cultivars resistant to arthropod attack.

Transcriptional profiling of catalase genes in juglone-treated seeds of maize (Zea mays L.) and wheat (Triticum aestivum L.).

The major aim of the present study was to investigate the influence of juglone (JU; 5-hydroxy-1,4-naphthoquinone) treatments on the expression level of Cat1, Cat2 and Cat3 genes, encoding the respective catalase isozymes in maize (Zea mays L.) and wheat (Triticum aestivum L.) seeds. In parallel, germination efficiency, catalase (CAT) activity and hydrogen peroxide (H2O2) content in juglone-exposed cereal seeds were assessed. Juglone applications significantly stimulated abundance of three target catalase transcripts as well as induced CAT activity and generation of H2O2 in both maize and wheat kernels. Furthermore, germination process of juglone-affected maize seeds was more severe suppressed than in case of wheat kernels. The role of juglone in triggering the oxidative stress as well as antioxidative responses in seeds of the studied model cereal species are discussed.

Tetraneura ulmi (Hemiptera: Eriosomatinae) Induces Oxidative Stress and Alters Antioxidant Enzyme Activities in Elm Leaves.

Gall formation is induced by an insect, which changes normal plant development and results in the formation of a new organ, following distinct stages of metabolic and developmental alterations. Research on mechanisms of recognition and responses to biotic stress may help to understand the interactions between galling aphids and their host plants. In this study, Tetraneura ulmi L. (Hemiptera: Eriosomatinae) galls and Ulmus pumila L. (Rosales: Ulmaceae) leaves were used as a model. Concentrations of hydrogen peroxide (H2O2) and thiobarbituric acid reactive substances, electrolyte leakage, as well as the activity of ascorbate peroxidase, guaiacol peroxidase, and catalase (CAT) were determined in galls and two parts of galled leaves (with and without visible damage). Biochemical analyses were performed at three stages of gall development: initial, fully developed, and mature galls. A slight increment in H2O2 content with a strong enhancement of ascorbate peroxidase and CAT activities were observed in galls and galled leaves in the first stage. In subsequent stages of gall development, a progressing increase in H2O2 production and cell membrane damage was associated with declining antioxidant enzyme activities, especially in gall tissues. The stages of gall development are likely to be part of cell death triggered by aphid feeding. It seems that the gall is the result of a biochemical struggle between the host plant and the gall inducer.

Parasitoid complex associated with the overwintering generation of Swammerdamia pyrella (Lepidoptera: Yponomeutidae) in Poland.

The study was conducted on fruit trees where bands of corrugated cardboard were attached around the trunks of the trees, which were used to catch the larvae of overwintering generation of the rufous-tipped swammerdamia moth, Swammerdamia pyrella (Villers) (Lepidoptera: Yponomeutidae). Twenty-five species of parasitic Hymenoptera have been described from S. pyrella in Poland including the report in this article of seven species belonging to the family of Ichneumonidae (three species) and superfamily Chalcidoidea (four species). The parasitoids Gelis agilis F. (Ichneumonidae), Chrysocharis aquilegiae (Erdös) (Eulophidae), Catolaccus ater (Ratzeburg) (Pteromalidae), and Eupelmus urozonus (Dalman) (Eupelmidae) had not been reported from the host before. Triclistus pallipes Holmgren (Ichneumonidae), Dibrachys cavus Walker (Pteromalidae) had the greatest effect on the natural regulation of S. pyrella population. Parasitization for the wintering cocoons of S. pyrella changed each year, but it was high throughout the study. The contribution of secondary parasitoids was much higher than primary parasitoids.