RESUMO
OBJECTIVE: The aim of this study was to investigate whether expanded instantaneous input dynamic ranges (IIDRs) in the Nucleus cochlear implant system benefit speech perception in the laboratory and listening in the real world. DESIGN: Until recently, Nucleus cochlear implants have used an IIDR of approximately 30 dB. In this study, an IIDR of 31 dB was compared with 46 dB and 56 dB in the SPEAR3 research processor with nine adult implant recipients. Subjects were given two, 2-wk blocks of take-home experience with each of the three IIDRs. A single IIDR setting was used in each trial period. During the take-home experience with the expanded IIDRs, subjects used two programs: a standard program (with clinically measured electrode dynamic ranges) and a program with adjusted thresholds (decreased T levels). After each block of take-home experience, speech perception testing was conducted for CNC words in quiet (at 45 dB and 55 dB SPL) and for CUNY sentences in the presence of multi-taker babble. RESULTS: On average, CNC word recognition at low presentation levels was significantly better with the 46 dB and 56 dB IIDRs, compared with the 31 dB IIDR; however, there was no significant difference between the 46 dB and 56 dB IIDR conditions. These benefits were greater for standard programs than for reduced T level programs. For CUNY sentences in babble, group results indicated no significant difference in performance across IIDR. The three IIDRs were rated similarly in real-life listening situations, and two of the subjects expressed tolerance problems with the expanded standard IIDRs. CONCLUSIONS: IIDRs of 46 and 56 dB provided benefit in accessing low-level speech without a decrement in sentence perception in babble. Most subjects accepted the standard, wider IIDR programs in everyday life. No significant differences were found between the 46 dB and 56 dB IIDR programs.
Assuntos
Implantes Cocleares , Núcleo Coclear/cirurgia , Estimulação Acústica/instrumentação , Idoso , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Desenho de Prótese , Ajuste de Prótese , Percepção da Fala , Inquéritos e QuestionáriosRESUMO
The glutathione redox couple is an information-rich redox buffer that interacts with numerous cellular components. To explore the role of glutathione in redox signalling, leaf contents were increased either chemically, by feeding reduced glutathione (GSH), or genetically, by over-expressing the first enzyme of the GSH biosynthetic pathway, gamma-glutamylcysteine synthetase (gamma-ECS). Leaf discs were also fed glutathione disulphide (GSSG), leading to increases in both GSH and GSSG. The effects of increases in GSH were compared with non-specific changes in leaf thiol status induced by feeding dithiothreitol (DTT) or the monothiol beta-mercaptoethanol (beta-ME). Photosynthesis measurements showed that none of the feeding treatments greatly disrupted leaf physiology. Transgenic plants expressing aequorin were used to analyse calcium signatures during the feeding treatments. Calcium release occurred soon after the onset of GSH or GSSG feeding, but was unaffected by DTT or beta-ME. Pathogenesis-related protein 1 (PR-1) was induced both in the gamma-ECS overexpressors and by feeding GSH, but not GSSG. Feeding DTT also induced PR-1. Key transcripts encoding antioxidative enzymes were much less affected, although glutathione synthetase was suppressed by feeding thiols or GSSG. It is concluded that modulation of glutathione contents transmits information through diverse signalling mechanisms, including (i) the establishment of an appropriate redox potential for thiol/disulphide exchange and (ii) the release of calcium to the cytosol.
Assuntos
Sinalização do Cálcio , Regulação da Expressão Gênica de Plantas , Glutationa/farmacologia , Dissulfeto de Glutationa/farmacologia , Oxirredução , Folhas de Planta/metabolismo , Plantas Geneticamente Modificadas , Compostos de Sulfidrila/farmacologiaRESUMO
Calcium signalling is little understood in filamentous fungi largely because easy and routine methods for calcium measurement in living hyphae have previously been unavailable. We have developed the recombinant aequorin method for this purpose. High levels of aequorin expression were obtained in Neurospora crassa, Aspergillus niger and Aspergillus awamori by codon optimization of the aequorin gene. Three external stimuli (mechanical perturbation, hypo-osmotic shock and high external calcium) were found transiently to increase [Ca(2+)](c). Each of the calcium signatures associated with these physico-chemical treatments was unique, suggesting the involvement of three distinct calcium-mediated signal transduction pathways. The fungal calcium channel blocker KP4 inhibited the [Ca(2+)](c) responses to hypo-osmotic shock and high external calcium, but not to mechanical perturbation. The divalent cation chelator BAPTA inhibited [Ca(2+)](c) responses to mechanical perturbation and hypo-osmotic shock. The calcium agonists A23187 and cyclopiazonic acid increased [Ca(2+)](c) levels.
Assuntos
Equorina/genética , Equorina/metabolismo , Aspergillus/metabolismo , Cálcio/metabolismo , Códon , Ácido Egtázico/análogos & derivados , Neurospora crassa/metabolismo , Aspergillus/citologia , Aspergillus/genética , Sequência de Bases , Calcimicina/metabolismo , Bloqueadores dos Canais de Cálcio/metabolismo , Sinalização do Cálcio/fisiologia , Quelantes/metabolismo , Ácido Egtázico/metabolismo , Inibidores Enzimáticos/metabolismo , Indóis/metabolismo , Ionóforos/metabolismo , Dados de Sequência Molecular , Neurospora crassa/citologia , Neurospora crassa/genética , Pressão Osmótica , Proteínas Recombinantes/genética , Proteínas Recombinantes/metabolismo , Alinhamento de Sequência , Estresse MecânicoRESUMO
Plant cell suspension cultures respond to osmotic changes by alterations in levels of free cellular calcium. Using the aequorin recombinant method, we have measured the spatial and temporal characteristics of calcium signatures in the nucleus and the cytosol of BY-2 tobacco suspension cells challenged with hypo- or hyper-osmotic shock. We show here that the nuclear compartment contributes together with the cytosol to produce calcium signal patterns that discriminate hypo- from hyper-osmotic treatments, i.e. turgor from tension. We also demonstrate that calcium responses in the nucleus and the cytosol are differentially modulated by the strength and the nature of hyper-osmotic treatments. We conclude that qualitative and quantitative changes in the parameters of an external stimulus such as osmotic changes are converted into calcium signatures, distinctive in their temporal and subcellular characteristics, involving both the nucleus and the cytosol. Our results illustrate the versatility of calcium signaling in plant cells. In addition to the physiological 'address' of the cell, the compartmentation of the calcium signal is probably an important parameter in encoding response specificity.
Assuntos
Bloqueadores dos Canais de Cálcio/farmacologia , Sinalização do Cálcio/fisiologia , Núcleo Celular/metabolismo , Citosol/metabolismo , Gadolínio/farmacologia , Sinalização do Cálcio/efeitos dos fármacos , Células Cultivadas , Pressão Osmótica , Nicotiana/citologiaRESUMO
Plants exhibit a variety of responses to abiotic stresses that enable them to tolerate and survive adverse conditions. As we learn more about the signalling pathways leading to these responses, it is becoming clear that they constitute a network that is interconnected at many levels. In this article, we discuss the 'cross-talk' between different signalling pathways and question whether there are any truly specific abiotic stress signalling responses.
Assuntos
Proteínas de Arabidopsis , Plantas/metabolismo , Receptor Cross-Talk , Transdução de Sinais , Ácido Abscísico/metabolismo , Sinalização do Cálcio , Temperatura Baixa , Desastres , Regulação da Expressão Gênica de Plantas , Reguladores de Crescimento de Plantas/metabolismo , Fenômenos Fisiológicos Vegetais , Plantas/genética , Regiões Promotoras Genéticas , Proteínas Serina-Treonina Quinases/metabolismo , Especificidade da Espécie , Estresse Mecânico , Fatores de Transcrição/metabolismoRESUMO
Currently, the only behind-the-ear hearing aid that provides a frequency transposition function is the ImpaCt DSR675, recently introduced by AVR Communications Ltd. of Israel. In tests with three hearing-impaired adults, the performance of the ImpaCt aid(s) was compared with that of each subject's own (nontransposing) hearing aids. Recognition of monosyllabic words and medial consonants did not differ significantly between the two types of aids. This suggests that the transposition function of the ImpaCt was not effective at providing these subjects with increased high-frequency speech information, at least for the programmable parameters applied in the experiments. However, the subjects' understanding of sentences in a competing noise was significantly poorer with the ImpaCt than with the subjects' own aids. In that test, the ImpaCt aids were programmed to attenuate parts of the noise. The decreased sentence recognition may have resulted from this program, which effectively reduced the bandwidth of the ImpaCt aids.
Assuntos
Auxiliares de Audição , Transtornos da Audição/terapia , Percepção da Fala/fisiologia , Estimulação Acústica/instrumentação , Idoso , Audiometria de Tons Puros , Limiar Auditivo/fisiologia , Desenho de Equipamento , Humanos , Pessoa de Meia-Idade , Fatores de TempoRESUMO
Cooling-induced 'calcium signatures' were imaged in aequorin-expressing Arabidopsis plants after cold acclimation or growth at ambient temperature. In all tissues, signatures were altered after acclimation. Characterization of the components generating this response indicates that cold acclimation increases cold-induced vacuolar Ca(2+) release, but does not affect the influx of extracellular calcium.
Assuntos
Adaptação Fisiológica , Arabidopsis/fisiologia , Cálcio/metabolismo , Temperatura Baixa , Arabidopsis/metabolismoRESUMO
Little is known about the signalling processes involved in the response of roots to abiotic stresses. The Arabidopsis root is a model system of root anatomy with a simple architecture and is amenable to genetic manipulation. Although it is known that the root responds to cold, drought and salt stress with increases in cytoplasmic free calcium, there is currently no information about the role(s) of the functionally diverse cell types that comprise the root. Transgenic Arabidopsis with enhancer-trapped GAL4 expression in specific cell types was used to target the calcium reporting protein, aequorin, fused to a modified yellow fluorescent protein (YFP). The luminescence output of targeted aequorin enabled in vivo measurement of changes in cytosolic free calcium concentrations ([Ca2+]cyt) in specific cell types during acute cold, osmotic and salt stresses. In response to an acute cold stress, all cell types tested as well as plants constitutively expressing aequorin displayed rapid [Ca2+]cyt peaks. However, there were significant quantitative differences between different cell types in terms of their response to cold stress, osmotic stress (440 mM mannitol) and salt stress (220 mM NaCl), implying specific roles for certain cell types in the detection and/or response to these stimuli. In response to osmotic and salt stress, the endodermis and pericycle displayed prolonged oscillations in cytosolic calcium that were distinct from the responses of the other cell types tested. Targeted expression of aequorin circumvented the technical difficulties involved in fluorescent dye injection as well as the lack of cell specificity of constitutively expressed aequorin, and revealed a new level of complexity in root calcium signalling.
Assuntos
Arabidopsis/fisiologia , Sinalização do Cálcio , Cálcio/metabolismo , Proteínas de Saccharomyces cerevisiae , Equorina/genética , Arabidopsis/citologia , Proteínas de Bactérias/genética , Temperatura Baixa , Citosol/metabolismo , Proteínas de Ligação a DNA , Desastres , Proteínas Fúngicas/genética , Regulação da Expressão Gênica de Plantas , Medições Luminescentes , Proteínas Luminescentes/genética , Concentração Osmolar , Raízes de Plantas/citologia , Raízes de Plantas/fisiologia , Plantas Geneticamente Modificadas , Proteínas Recombinantes de Fusão/biossíntese , Transdução de Sinais , Fatores de Transcrição/genéticaRESUMO
Cold shock and wind stimuli initiate Ca(2+) transients in transgenic tobacco (Nicotiana plumbaginifolia) seedlings (named MAQ 2.4) containing cytoplasmic aequorin. To investigate whether these stimuli initiate Ca(2+) pathways that are spatially distinct, stress-induced nuclear and cytoplasmic Ca(2+) transients and the expression of a stress-induced calmodulin gene were compared. Tobacco seedlings were transformed with a construct that encodes a fusion protein between nucleoplasmin (a major oocyte nuclear protein) and aequorin. Immunocytochemical evidence indicated targeting of the fusion protein to the nucleus in these plants, which were named MAQ 7.11. Comparison between MAQ 7.11 and MAQ 2.4 seedlings confirmed that wind stimuli and cold shock invoke separate Ca(2+) signaling pathways. Partial cDNAs encoding two tobacco calmodulin genes, NpCaM-1 and NpCaM-2, were identified and shown to have distinct nucleotide sequences that encode identical polypeptides. Expression of NpCaM-1, but not NpCaM-2, responded to wind and cold shock stimulation. Comparison of the Ca(2+) dynamics with NpCaM-1 expression after stimulation suggested that wind-induced NpCaM-1 expression is regulated by a Ca(2+) signaling pathway operational predominantly in the nucleus. In contrast, expression of NpCaM-1 in response to cold shock is regulated by a pathway operational predominantly in the cytoplasm.
Assuntos
Cálcio/fisiologia , Calmodulina/genética , Regulação da Expressão Gênica de Plantas , Nicotiana/genética , Nicotiana/metabolismo , Plantas Tóxicas , Transdução de Sinais/fisiologia , Equorina/biossíntese , Equorina/genética , Sequência de Bases , Calmodulina/química , Genes de Plantas , Dados de Sequência Molecular , Plantas Geneticamente Modificadas , Isoformas de Proteínas/química , Isoformas de Proteínas/genética , Proteínas Recombinantes/biossíntese , Proteínas Recombinantes/química , Alinhamento de Sequência , Homologia de Sequência de AminoácidosAssuntos
Inibidores da Enzima Conversora de Angiotensina/economia , Inibidores da Enzima Conversora de Angiotensina/uso terapêutico , Hipertensão/tratamento farmacológico , Hipertensão/economia , Indigência Médica/economia , Adulto , Inibidores da Enzima Conversora de Angiotensina/efeitos adversos , Benzazepinas/economia , Benzazepinas/uso terapêutico , Pressão Sanguínea/efeitos dos fármacos , Humanos , Hipertensão/fisiopatologia , Lisinopril/economia , Lisinopril/uso terapêuticoRESUMO
Cold elicits an immediate rise in the cytosolic free calcium concentration ([Ca2+]c) of plant cells. We have studied the concerted action of the three underlying mechanisms, namely sensing, sensitisation and desensitisation, which become important when plants in the field are subjected to changes in temperature. We applied different regimes of temperature changes with well-defined cooling rates to intact roots of Arabidopsis thaliana expressing the calcium-indicator, aequorin. Our results indicate that temperature sensing is mainly dependent on the cooling rate, dT/dt, whereas the absolute temperature T is of less importance. Arabidopsis roots were found to be sensitive to cooling rates of less than dT/dt = 0.01 degrees C/s. However, at cooling rates below 0.003 degrees C/s (i.e. cooling 10 degrees C in 1 h) there is no detectable [Ca2+]c response at all. At low temperature, the sensitivity of the plant cold-detection system is increased. This in turn produces greater cooling-induced [Ca2+]c elevations. Prolonged or repeated cold treatment attenuates the [Ca2+]c responses to subsequent episodes of cooling.
Assuntos
Arabidopsis/fisiologia , Sinalização do Cálcio , Temperatura Baixa , Raízes de Plantas/fisiologia , Equorina/genética , Equorina/metabolismo , Periodicidade , Proteínas Recombinantes/metabolismoRESUMO
Aluminium, the most abundant metal in the earth's crust, is highly toxic to most plant species. One of the prevailing dogmas is that aluminium exerts this effect by disrupting cellular calcium homeostasis. However, recent research gives strongly conflicting results: aluminium was shown to provoke either an increase or a decrease in cytosolic free calcium concentration ([Ca2+]c). To solve this question, we have adopted a novel approach: [Ca2+]c measurements in intact plant roots as opposed to isolated cells, and the correlative measurements of intracellular and external pH. The results obtained show that plant roots respond to low external pH by a sustained elevation in [Ca2+]c. In the presence of aluminium, this pH-mediated elevation in [Ca2+]c does not occur, therefore any potential calcium-mediated protection against low pH is likely to be irreversibly inhibited. The severity of the inhibitory effect of aluminium on [Ca2+]c depends on the concentration of external calcium, thus perhaps explaining why the effects of aluminium toxicity are ameliorated in calcium-rich soils. It seems possible that a primary toxic effect of aluminium might be to impair calcium-mediated plant defence responses against low pH.
Assuntos
Alumínio/toxicidade , Cálcio/metabolismo , Citosol/metabolismo , Concentração de Íons de Hidrogênio , Equorina/genética , Arabidopsis/metabolismo , Bloqueadores dos Canais de Cálcio/toxicidade , Raízes de Plantas/metabolismo , Plantas Geneticamente Modificadas/metabolismoRESUMO
The sfr mutations, which result in sensitivity to freezing after cold acclimation, define genes that are required for freezing tolerance. We tested plants homozygous for mutations sfr2 to sfr7 for cold-induced gene expression and found that sfr 6 plants were deficient in cold-inducible expression of the genes KIN1, COR15a, and LTI78, which all contain the C repeat/dehydration-responsive element (CRT/DRE) motif in their promoters. Similarly, sfr 6 plants failed to induce KIN1 normally in response to either osmotic stress or the application of abscisic acid. In contrast, cold-inducible expression of genes CBF1, CBF2, CBF3, and ATP5CS1, which lack the CRT/DRE motif, was not affected. The freezing-sensitive phenotype that defines sfr 6 also was found to be tightly linked to the gene expression phenotype. To determine whether the failure of cold induction of CRT/DRE-containing genes in sfr 6 was due to altered low-temperature calcium signaling, cold-induced cytosolic-free calcium ([Ca2+]cyt) elevations were investigated in the sfr 6 mutant, but these were found to be indistinguishable from those of the wild type. We discuss the possibilities that CRT/DRE binding proteins (such as CBF1) require activation to play a role in transcription and that the SFR6 protein is a vital component of their activation.
Assuntos
Aclimatação/genética , Proteínas de Arabidopsis , Arabidopsis/genética , Congelamento , Regulação da Expressão Gênica de Plantas , Mutação , Elementos de Resposta , Fatores de Transcrição , 1-Pirrolina-5-Carboxilato Desidrogenase , Cálcio/metabolismo , Mapeamento Cromossômico , Cruzamentos Genéticos , Proteínas de Ligação a DNA/biossíntese , Oxirredutases atuantes sobre Doadores de Grupo CH-NH/biossíntese , Fosfoproteínas/genética , Proteínas de Plantas/genética , Proteínas Serina-Treonina Quinases/genética , Transdução de Sinais , Transativadores/biossíntese , Transcrição Gênica , Água/metabolismoRESUMO
The cellular responses of plants to numerous extracellular stimuli are mediated by transient elevations in the concentration of cytosolic free calcium ([Ca2+]cyt). We have addressed the question of how cells can use this apparently ubiquitous system to initiate so many specific and appropriate end responses. We show that the pollutant gas ozone elicits a biphasic Ca2+ response in intact Arabidopsis plants and a subsequent increase in expression of the gene encoding the antioxidant defence enzyme glutathione-S-transferase (GST). The second of the two [Ca2+]cyt peaks, but not the first, could be eliminated either by pre-treatment of plants with lanthanum chloride, or by reducing the duration of ozone fumigation. Under these conditions, ozone-induced GST expression was abolished. These data provide a functional dissection of the ozone Ca2+ signalling pathway and indicate that the second ozone-induced [Ca2+]cyt peak provides the necessary information to direct expression of GST.
Assuntos
Cálcio/metabolismo , Ozônio/farmacologia , Arabidopsis/enzimologia , Arabidopsis/metabolismo , Sequência de Bases , Primers do DNA , Regulação Enzimológica da Expressão Gênica/efeitos dos fármacos , Regulação da Expressão Gênica de Plantas/efeitos dos fármacos , Glutationa Transferase/genética , Glutationa Transferase/metabolismo , RNA Mensageiro/genéticaRESUMO
The purpose of this phenomenological study was to describe the lived experience of patient prudence in health care. Prudence has previously been defined as good judgement in setting realistic personal goals and using personal resources to achieve those goals. Audiotaped interviews were conducted with 10 hospitalized adults for whom health care providers had previously recommended life style changes for health reasons. Data were analysed using Colaizzi's method. Seventy-seven significant statements were identified and, from their formulated meanings, seven themes emerged that were integrated into a description of the fundamental structure of prudence. From the patient perspective, prudence in health care is a dynamic phenomenon that involves achieving well-being and self-perpetuation within the context of the patient's world of competing values and is experienced with emotions that range from harmony to fear and depression.
Assuntos
Comportamentos Relacionados com a Saúde , Participação do Paciente , Adulto , Idoso , Feminino , Humanos , Pacientes Internados , Masculino , Pessoa de Meia-Idade , Estados UnidosRESUMO
Environmental stresses commonly encountered by plants lead to rapid transient elevations in cytosolic free calcium concentration ([Ca2+]cyt) (Bush, 1995; Knight et al., 1991). These cellular calcium (Ca2+) signals lead ultimately to the increased expression of stress-responsive genes, including those encoding proteins of protective function (Knight et al., 1996; Knight et al., 1997). The kinetics and magnitude of the Ca2+ signal, or 'calcium signature', differ between different stimuli and are thought to contribute to the specificity of the end response (Dolmetsch et al., 1997; McAinsh and Hetherington, 1998). We measured [Ca2+]cyt changes during treatment with mannitol (to mimic drought stress) in whole intact seedlings of Arabidopsis thaliana. The responses of plants which were previously exposed to osmotic and oxidative stresses were compared to those of control plants. We show here that osmotic stress-induced Ca2+ responses can be markedly altered by previous encounters with either osmotic or oxidative stress. The nature of the alterations in Ca2+ response depends on the identity and severity of the previous stress: oxidative stress pre-treatment reduced the mannitol-induced [Ca2+]cyt response whereas osmotic stress pretreatment increased the [Ca2+]cyt response. Therefore, our data show that different combinations of environmental stress can produce novel Ca2+ signal outputs. These alterations are accompanied by corresponding changes in the patterns of osmotic stress-induced gene expression and, in the case of osmotic stress pre-treatment, the acquisition of stress-tolerance. This suggests that altered Ca2+ responses encode a 'memory' of previous stress encounters and thus may perhaps be involved in acclimation to environmental stresses.
Assuntos
Arabidopsis/fisiologia , Cálcio/metabolismo , Estresse Oxidativo/fisiologia , Transdução de Sinais/fisiologia , Equorina/genética , Equorina/fisiologia , Arabidopsis/efeitos dos fármacos , Clima , Cinética , Manitol/farmacologia , Concentração Osmolar , Plantas Geneticamente Modificadas , Proteínas Recombinantes/biossínteseRESUMO
Hypoosmotic shock treatment increased cytosolic Ca2+ ion concentration ([Ca2+]cyt) in tobacco (Nicotiana tabacum) suspension-culture cells. [Ca2+]cyt measurements were made by genetically transforming these cells to express apoaequorin and by reconstituting the Ca2+-dependent photoprotein, aequorin, in the cytosol by incubation with chemically synthesized coelenterazine. Measurement of Ca2+-dependent luminescence output thus allowed the direct monitoring of [Ca2+]cyt changes. When cells were added to a hypoosmotic medium, a biphasic increase in [Ca2+]cyt was observed; an immediate small elevation (phase 1) was observed first, followed by a rapid, large elevation (phase 2). Phase 1 [Ca2+]cyt was stimulated by the V-type ATPase inhibitor bafilomycin A1. Phase 2 was inhibited by the protein kinase inhibitor K-252a and required the continued presence of the hypoosmotic stimulus to maintain it. Although Ca2+ in the medium was needed to produce phase 2, it was not needed to render the cells competent to the hypoosmotic stimulus. If cells were subject to hypoosmotic shock in Ca2+- depleted medium, increases in luminescence could be induced up to 20 min after the shock by adding Ca2+ to the medium. These data suggest that hypoosmotic shock-induced [Ca2+]cyt elevation results from the activity of a Ca2+ channel in the plasma membrane or associated hypoosmotic sensing components that require Ca2+- independent phosphorylation and a continued stimulus to maintain full activity.
RESUMO
Using Nicotiana plumbaginifolia constitutively expressing the recombinant bioluminescent calcium indicator, aequorin, it has been previously demonstrated that plant cells react to cold-shock by an immediate rise in cytosolic calcium. Such an opportune system has been exploited to address the regulatory pathway involved in the calcium response. For this purpose, we have used protoplasts derived from N. plumbaginifolia leaves that behave as the whole plant but with a better reproducibility. By both immunodetecting cytoskeletal components on membrane ghosts and measuring the relative change in cytosolic calcium, we demonstrate that the organization of the cytoskeleton has profound influences on the calcium response. The disruption of the microtubule meshwork by various active drugs, such as colchicin, oryzalin and vinblastin, leads to an important increase in the cytosolic calcium (up to 400 nM) in cold-shocked protoplasts over control. beta-Lumicolchicin, an inactive analogue of colchicin, is ineffective either on cytoplasmic calcium increase or on microtubule organization. A microfilament disrupting drug, cytochalasin D, exerts a slight stimulatory effect, whereas the simultaneous disruption of microtubule and microfilament meshworks results in a dramatic increase in the calcium response to cold-shock. The results described in the present paper illustrate the role of the intracellular organization and, more specifically, the role of cytoskeleton in controlling the intensity of calcium response to an extracellular stimulus.
Assuntos
Citoesqueleto de Actina/metabolismo , Cálcio/metabolismo , Temperatura Baixa , Microtúbulos/metabolismo , Protoplastos/metabolismo , Citoesqueleto/metabolismo , Citosol/metabolismo , Plantas Tóxicas , NicotianaRESUMO
Changes in cytosolic free calcium concentration ([Ca2+]cyt) in response to mannitol (drought) and salt treatments were detected in vivo in intact whole Arabidopsis seedlings. Transient elevations of [Ca2+]cyt to around 1.5 microM were observed, and these were substantially inhibited by pretreatment with the calcium-channel blocker lanthanum and to a lesser extent, the calcium-chelator EGTA. The expression of three genes, p5cs, which encodes delta(1)-pyrroline-5-carboxylate synthetase (P5CS), the first enzyme of the proline biosynthesis pathway, rab18 and Iti78 which both encode proteins of unknown function, was induced by mannitol and salt treatments. The induction of all three genes by mannitol was inhibited by pretreatment with lanthanum. Salt-induced p5cs, but not rab18 and Iti78, expression was also inhibited by lanthanum. Induction of p5cs by mannitol was also inhibited by the calcium channel-blockers gadolinium and verapamil and the calcium chelator EGTA, further suggesting the involvement of calcium signalling in this response. Mannitol induced greater levels of p5cs gene expression than an isoosmolar concentration of salt, at both relatively high and low concentrations. However, calcium transients were of a similar magnitude and duration in response to both mannitol and isoosmolar concentrations of salt, suggesting that a factor other than calcium is involved in the discrimination between drought and salinity signals in Arabidopsis. In order to gauge the involvement of the vacuole as an intracellular calcium store in the response of Arabidopsis to mannitol, [Ca2+]cyt was measured at the microdomain adjacent to the vacuolar membrane. The results obtained were consistent with a significant calcium release from the vacuole contributing to the overall mannitol-induced [Ca2+]cyt response. Data obtained by using inhibitors of inositol signalling suggested that this release was occurring through IP3-dependent calcium channels.