RESUMO
A reversed passive hemagglutination test was developed to assay relative concentrations of soluble antigen of Legionnaires disease (Legionella pneumophila serogroup 1) in human urine samples. The test is highly sensitive, being able to detect as little as 0.0002 microgram of total antigen. Preliminary results with this test on serial urine and serum samples from a patient with legionellosis show that measurable amounts of antigen are present in urine during the course of the illness. However, no antigen could be detected in the serum of the patient.
Assuntos
Antígenos de Bactérias/análise , Testes de Hemaglutinação/métodos , Legionella/imunologia , Urina/imunologia , Humanos , Doença dos Legionários/diagnóstico , Doença dos Legionários/imunologiaAssuntos
Cádmio/análise , Rim/análise , Fígado/análise , Adolescente , Adulto , Idoso , Inglaterra , Exposição Ambiental , Feminino , Humanos , Masculino , Pessoa de Meia-Idade , Medicina do Trabalho , EspectrofotometriaRESUMO
Reversed passive hemagglutination was used to assay enterotoxin in culture filtrates and in food samples. With cells tanned and then sensitized with antitoxin globulin and preserved with either formaldehyde or pyruvic aldehyde, as little as 0.0007 mug of enterotoxin was detectable. The results of hemagglutination tests compared well with those obtained by quantitative precipitin tests or by immunodiffusion, but hemagglutination was 50 to 100 times more sensitive than the immunodiffusion technique. In addition, results of the hemagglutination test were available within a few hours, and neither elimination of interfering proteins from food extracts nor concentration of the sample, both of which are necessary for immunodiffusion, was required for this procedure.
