RESUMO
We studied in vitro effects of recombinant interleukin-5, interleukin-3, and eotaxin on programmed death of eosinophils from healthy donors and patients with non-Hodgkin's lymphomas associated with severe blood eosinophilia. Interleukin-5 and eotaxin produced the most potent antiapoptotic effect on eosinophils from healthy donors. In patients with non-Hodgkin's lymphomas, spontaneous apoptosis in eosinophilic leukocytes was low and remained unchanged during incubation with recombinant proteins.
Assuntos
Apoptose/efeitos dos fármacos , Quimiocinas CC/farmacologia , Eosinófilos/efeitos dos fármacos , Interleucina-3/farmacologia , Interleucina-5/farmacologia , Proteínas Recombinantes/farmacologia , Adolescente , Adulto , Estudos de Casos e Controles , Células Cultivadas , Quimiocinas CC/genética , Quimiocinas CC/metabolismo , Eosinofilia/sangue , Eosinófilos/citologia , Feminino , Humanos , Interleucina-3/genética , Interleucina-3/metabolismo , Interleucina-5/genética , Interleucina-5/metabolismo , Linfoma não Hodgkin/sangue , Masculino , Pessoa de Meia-Idade , Proteínas Recombinantes/metabolismoRESUMO
The researchers evaluated the condition of lipid peroxidation and antioxidant protection system together with biochemical, immunological and morphological values in 117 patients with chronic hepatitis (CH) in virus replication phase (28 patient had CH type B, 66--CH type C and 28--CH type B+C), and estimated the importance of their interrelations to the prediction of disease outcomes.
Assuntos
Antioxidantes/metabolismo , Hepatite B Crônica/metabolismo , Hepatite C Crônica/metabolismo , Peroxidação de Lipídeos , Adolescente , Adulto , Idoso , Feminino , Hepatite B Crônica/patologia , Hepatite C Crônica/patologia , Humanos , Masculino , Pessoa de Meia-Idade , Valor Preditivo dos Testes , Resultado do TratamentoRESUMO
AIM: To reveal the structural features of the red blood cell membrane in patients with prolonged persistence of hepatitis B virus (HBV) and hepatitis C virus (HCV). MATERIALS AND METHODS: 61 patients with moderate and mild chronic viral hepatitis B and C were examined. A control group comprised 22 healthy donors. Fluorescence of the red blood cell membrane was performed, by using the following probes: pyrene, phenylnaphthylamine, 1-anilinonaphthalene-8-sulfonate. The activity of the ion-transporting enzyme Na+, K(+)-ATPase was determined. RESULTS: Increases in the microviscosity of the lipid bilayer, periprotein lipid environment, as well as the structural modification of the surface layers of the membrane were found in patients with chronic hepatitis B and C. The activity of the membrane-bound enzyme Na+, K(+)-ATPase was statistically significantly decreased as compared with that in healthy donors. CONCLUSION: In HBV and HCV infection, the impairments of the red blood system are highly diversified: they involve bone marrow compartment of an erythron, as earlier shown, and its peripheral one. Molecular disorganization of the red blood cell membrane fails to present with obvious red blood cell dysfunctions. Despite the multifactorial property of their occurrence, impairments of the red blood system are mediated and corrected by many regulatory mechanisms at different organizational levels and may be compensated.
