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1.
Br J Pharmacol ; 175(10): 1580-1589, 2018 05.
Artigo em Inglês | MEDLINE | ID: mdl-29235092

RESUMO

BACKGROUND AND PURPOSE: Bumetanide has anxiolytic effects in rat models of conditioned fear. As a loop diuretic, bumetanide blocks cation-chloride co-transport and this property may allow bumetanide to act as an anxiolytic by modulating GABAergic synaptic transmission in the CNS. Its potential for the treatment of anxiety disorders deserves further investigation. In this study, we evaluated the possible involvement of the basolateral nucleus of the amygdala in the anxiolytic effect of bumetanide. EXPERIMENTAL APPROACH: Brain slices were prepared from Wistar rats. extracellular recording, stereotaxic surgery, fear-potentiated startle response, locomotor activity monitoring and Western blotting were applied in this study. KEY RESULTS: Systemic administration of bumetanide (15.2 mg·kg-1 , i.v.), 30 min prior to fear conditioning, significantly inhibited the acquisition of the fear-potentiated startle response. Phosphorylation of ERK in the basolateral nucleus of amygdala was reduced after bumetanide administration. In addition, suprafusion of bumetanide (5 or 10 µM) attenuated long-term potentiation in the amygdala in a dose-dependent manner. Intra-amygdala infusion of bumetanide, 15 min prior to fear conditioning, also blocked the acquisition of the fear-potentiated startle response. Finally, the possible off-target effect of bumetanide on conditioned fear was excluded by side-by-side control experiments. CONCLUSIONS AND IMPLICATIONS: These results suggest the basolateral nucleus of amygdala plays a critical role in the anxiolytic effects of bumetanide.


Assuntos
Bumetanida/farmacologia , Condicionamento Clássico/efeitos dos fármacos , Medo/efeitos dos fármacos , Atividade Motora/efeitos dos fármacos , Reflexo de Sobressalto/efeitos dos fármacos , Animais , Masculino , Ratos , Ratos Wistar
2.
Psychopharmacology (Berl) ; 232(20): 3809-19, 2015 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-26282370

RESUMO

RATIONALE: The involvement of glutamate in fear extinction is perhaps the most promising in terms of facilitating clinical interventions for posttraumatic stress disorder (PTSD). This study was aimed at elucidating the possible role of zif268 on the D-cycloserine (DCS) facilitation effect on extinction. OBJECTIVE: We investigated the association between zif268 and the extinction of conditioned fear by using antisense oligodeoxynucleotide (ODN) of zif268 and the fear-potentiated startle paradigm. METHODS: Male adult Wistar rats were injected DCS (15 mg/kg, IP) 15 min prior to the extinction training, administered with antisense or sense ODN (800 pmol) of zif268 and subjected for fear-potentiated startle paradigm (FPS) and Western blot. RESULTS: Either context exposure or cue exposure elevated the expression of zif268 in the basolateral nucleus of the amygdala (BLA) (p < 0.05 and p < 0.01, respectively) compared to the control group. Additionally, zif268 expression in BLA was further elevated by the glutamate NMDA receptor agonist DCS administration. Intra-amygdaloid injection of the antisense ODN of zif268 blocked the facilitation effect of DCS on the extinction of conditioned fear. Subsequent control experiments indicated that administration of vehicle or zif268 sense ODN did not alter the facilitation of DCS and that the blockage effect of zif268 antisense ODN was not due to lasting damage to the amygdala. CONCLUSIONS: Our results suggest that zif268 within the amygdala participates in the DCS facilitation effect on the extinction of conditioned fear.


Assuntos
Tonsila do Cerebelo/metabolismo , Condicionamento Psicológico/fisiologia , Ciclosserina/administração & dosagem , Proteína 1 de Resposta de Crescimento Precoce/biossíntese , Extinção Psicológica/fisiologia , Medo/fisiologia , Tonsila do Cerebelo/efeitos dos fármacos , Animais , Condicionamento Psicológico/efeitos dos fármacos , Agonistas de Aminoácidos Excitatórios/administração & dosagem , Extinção Psicológica/efeitos dos fármacos , Medo/efeitos dos fármacos , Medo/psicologia , Ácido Glutâmico/metabolismo , Injeções Intraventriculares , Masculino , Ratos , Ratos Sprague-Dawley , Ratos Wistar , Receptores de N-Metil-D-Aspartato/metabolismo
3.
PLoS One ; 9(11): e106692, 2014.
Artigo em Inglês | MEDLINE | ID: mdl-25369049

RESUMO

The loop diuretic bumetanide (Bumex) is thought to have antiepileptic properties via modulate GABAA mediated signaling through their antagonism of cation-chloride cotransporters. Given that loop diuretics may act as antiepileptic drugs that modulate GABAergic signaling, we sought to investigate whether they also affect hippocampal function. The current study was performed to evaluate the possible role of NKCC1 on the hippocampal function. Brain slice extracellular recording, inhibitory avoidance, and western blot were applied in this study. Results showed that hippocampal Long-term potentiation was attenuated by suprafusion of NKCC1 inhibitor bumetanide, in a dose dependent manner. Sequent experiment result showed that Intravenous injection of bumetanide (15.2 mg/kg) 30 min prior to the training session blocked inhibitory avoidance learning significantly. Subsequent control experiment's results excluded the possible non-specific effect of bumetanide on avoidance learning. We also found the phosphorylation of hippocampal MAPK was attenuated after bumetanide administration. These results suggested that hippocampal NKCC1 may via MAPK signaling cascade to possess its function.


Assuntos
Anticonvulsivantes/farmacologia , Aprendizagem da Esquiva/efeitos dos fármacos , Hipocampo/efeitos dos fármacos , Potenciação de Longa Duração/efeitos dos fármacos , Membro 2 da Família 12 de Carreador de Soluto/metabolismo , Animais , Bumetanida/farmacologia , Hipocampo/metabolismo , Masculino , Memória de Longo Prazo/efeitos dos fármacos , Proteínas Quinases Ativadas por Mitógeno/metabolismo , Atividade Motora/efeitos dos fármacos , Fosforilação/efeitos dos fármacos , Picrotoxina/farmacologia , Ratos , Ratos Wistar , Receptores de GABA-A/metabolismo , Transdução de Sinais/efeitos dos fármacos , Membro 2 da Família 12 de Carreador de Soluto/química
4.
Int J Neuropsychopharmacol ; 17(12): 1995-2004, 2014 Dec.
Artigo em Inglês | MEDLINE | ID: mdl-24945924

RESUMO

Synthetic glucocorticoid dexamethasone (DEX) is frequently used as a therapeutic agent to lessen the morbidity of chronic lung disease in premature infants. Previous studies suggested that neonatal DEX treatment altered brain development and cognitive function. It has been recognized that the amygdala is involved in emotional processes and also a critical site of neuronal plasticity for fear conditioning. Little is known about the possible long-term adverse effect of neonatal DEX treatment on amygdala function. The present study was aimed to evaluate the possible effect of neonatal DEX treatment on the synaptic function of amygdala in adult rats. Newborn Wistar rats were subjected to subcutaneous tapering-dose injections of DEX (0.5, 0.3 and 0.1 mg/kg) from post-natal day one to three, PN1-PN3. Animals were then subjected to a forced swimming test (FST) and electrophysiological recording aged eight weeks. The results of the FST showed neonatal DEX treatment increased depression-like behaviour in adulthood. After acute stress evoking, the percentage of time spent free floating is significantly increased in the DEX treated group compared with the control animals. Furthermore, neonatal DEX treatment elevated long-term potentiation (LTP) response and the phosphorylation level of MAPK in the lateral nucleus of amygdala (LA). Intracerebroventricular infusion of the MAPK inhibitor, PD98059, showed significant rescue effects including reduced depression-like behaviour and restoration of LTP to within normal range. In conclusion, our results suggested that MAPK signalling cascade in the LA plays an important role in the adverse effect of neonatal DEX treatment on amygdala function, which may result in adverse consequences in adult age, such as the enhancement of susceptibility for a depressive disorder in later life.


Assuntos
Tonsila do Cerebelo/efeitos dos fármacos , Tonsila do Cerebelo/crescimento & desenvolvimento , Transtorno Depressivo/induzido quimicamente , Transtorno Depressivo/fisiopatologia , Glucocorticoides/toxicidade , Tonsila do Cerebelo/fisiopatologia , Animais , Animais Recém-Nascidos , Antidepressivos/farmacologia , Transtorno Depressivo/tratamento farmacológico , Relação Dose-Resposta a Droga , Flavonoides/farmacologia , Potenciação de Longa Duração/efeitos dos fármacos , Potenciação de Longa Duração/fisiologia , Masculino , Proteínas Quinases Ativadas por Mitógeno/antagonistas & inibidores , Proteínas Quinases Ativadas por Mitógeno/metabolismo , Fosforilação/efeitos dos fármacos , Inibidores de Proteínas Quinases/farmacologia , Distribuição Aleatória , Ratos Wistar , Estresse Psicológico/fisiopatologia , Técnicas de Cultura de Tecidos
5.
Neurosci Lett ; 512(2): 109-13, 2012 Mar 23.
Artigo em Inglês | MEDLINE | ID: mdl-22342305

RESUMO

In ray-finned fishes, the lateral (Dl) and medial (Dm) division of the dorsal telencephalon are important in learning and memory formation. Tract-tracing studies revealed that neural connections are formed between these regions via afferent Dl fibers projecting to the Dm. However, research analyzing Dl-Dm synaptic transmission is scant. We have used electrophysiological techniques to assess neurotransmission and synaptic plasticity in projections from the Dl to the Dm in zebrafish. We demonstrate that electrical stimulation of the Dl division evoked a negative field potential in the Dm division that could be inhibited by application of the AMPA/kainate receptor antagonist, CNQX (5µM). Pairs of stimuli, when delivered at brief inter-pulse intervals (IPI), elicited paired pulse facilitation (PPF). Long-term potentiation (LTP), induced through the application of three trains of high frequency stimulation (HFS; 100Hz for 1s), lasted for more than 1h and could be inhibited with DL-AP5 (40µM), an N-methyl-d-aspartate (NMDA) receptor antagonist. Our results suggest that the intratelencephalic connection between Dl and Dm may play an important role in the synaptic plasticity of the zebrafish brain. It also provides a new electrophysiological model for studying the neural mechanisms underlying learning and memory in zebrafish.


Assuntos
Plasticidade Neuronal , Telencéfalo/fisiologia , 2-Amino-5-fosfonovalerato/farmacologia , Animais , Estimulação Elétrica , Potenciais Evocados , Feminino , Potenciação de Longa Duração/efeitos dos fármacos , Masculino , Receptores de N-Metil-D-Aspartato/antagonistas & inibidores , Transmissão Sináptica , Peixe-Zebra
6.
J Agric Food Chem ; 56(16): 6910-3, 2008 Aug 27.
Artigo em Inglês | MEDLINE | ID: mdl-18616261

RESUMO

Resveratrol is a natural polyphenol and possesses many biological functions such as anti-inflammatory activity and protection against atherosclerosis and myocardial infraction. Parkinson's disease is a common progressive neurodegenerative disease. 1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) is the most useful neurotoxin to induce Parkinsonism. The present study was carried out to elucidate the neuroprotective effect and possible mechanism of resveratrol on MPTP-induced striatal neuron loss. Sixty adult Balb/c mice were divided into four groups: sham operation, MPTP treatment (30 mg/kg, i.p.), MPTP combined with resveratrol administration (20 mg/kg, i.v.), and resveratrol treatment alone. Microdialysis and high-performance liquid chromatography were used to analyze dihydroxybenzoic acid (DHBA) that reflected the hydroxyl radical level. In the present study, we found MPTP chronic administration significantly induced motor coordination impairment in mice. After MPTP administration, the hydroxyl radical levels in substantia nigra were also significantly elevated and animals displayed severe neuronal loss. Resveratrol administration significantly protected mice from MPTP-induced motor coordination impairment, hydroxyl radical overloading, and neuronal loss. Our results demonstrated that resveratrol could elicit neuroprotective effects on MPTP-induced Parkinsonism through free radical scavenging.


Assuntos
1-Metil-4-Fenil-1,2,3,6-Tetra-Hidropiridina/administração & dosagem , Sequestradores de Radicais Livres/farmacologia , Neurônios/efeitos dos fármacos , Fármacos Neuroprotetores/farmacologia , Estilbenos/farmacologia , Animais , Ataxia/induzido quimicamente , Ataxia/prevenção & controle , Corpo Estriado/citologia , Força da Mão , Masculino , Camundongos , Camundongos Endogâmicos BALB C , Atividade Motora/efeitos dos fármacos , Doença de Parkinson Secundária/induzido quimicamente , Doença de Parkinson Secundária/prevenção & controle , Resveratrol
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