1.
FEBS Lett
; 311(3): 206-8, 1992 Oct 26.
Artigo
em Inglês
| MEDLINE
| ID: mdl-1356832
RESUMO
The urocanase gene was detected in a clone obtained from a genomic library of white clover. The entire gene has been sequenced and expressed in the pT7-7/E. coli BL 21 (DE 3) system. The deduced sequence of the plant urocanase is 72% homologous with that of the well-characterized urocanase from Pseudomonas putida. The purification procedure, as well as kinetic and electrophoretic behaviour, of the new enzyme are described.