RESUMO
In search for new targets for obesity treatment, we have studied the effect of several transcription factors on the conversion of murine preadipocytes from the 3T3-L1 cell line into adipocytes. We have found that knockdown of Prep1 gene expression affects adipogenic differentiation and results in significant increase in the insulin-sensitive glucose carrier Glut4 gene expression.
Assuntos
Adipócitos/metabolismo , Regulação da Expressão Gênica/fisiologia , Transportador de Glucose Tipo 4/metabolismo , Proteínas de Homeodomínio/metabolismo , Transcrição Gênica/fisiologia , Células 3T3-L1 , Animais , Western Blotting , Diferenciação Celular/fisiologia , Técnicas de Silenciamento de Genes , Proteínas de Homeodomínio/genética , Camundongos , Reação em Cadeia da Polimerase , Células-Tronco/metabolismoRESUMO
BACKGROUND: Low adiponectin concentration observed in obese patients is associated with a high risk of metabolic disorders and cardiovascular diseases and could be related to single nucleotide polymorphisms (SNPs) in T-cadherin gene (CDH13). T-cadherin is a receptor for adiponectin and low-density lipoprotein. Aim of this study was to investigate association of CDH13 SNPs with the development of obesity in patients with ischemic heart disease (IHD). RESULTS: We established a statistically significant correlation between the number of minor alleles of rs11646213, rs4783244 and rs12444338 in CDH13 gene with body mass index: patients with smaller number of minor alleles tended to have normal body weight (odds ratio 3.03, 95% confidence interval 1.03-8.87). CONCLUSION: The obtained results are indicative of the cumulative effect of SNPs in CDH13 (rs11646213, rs4783244, rs12444338) on BMI in patients with IHD.
Assuntos
Isquemia Miocárdica , Polimorfismo de Nucleotídeo Único , Alelos , Índice de Massa Corporal , Caderinas , Doença da Artéria Coronariana , Genótipo , Humanos , Lipoproteínas LDL , ObesidadeRESUMO
To enhance stress resistance in pupils is now a very urgent problem. The influence of a method affecting the central nervous system as acquisition of the averaged value of intrinsic respiratory rhythm has been studied. There has been a positive result of using the procedure to increase working capacity during performance by pupils and positive changes in electroencephalographic readings, by optimizing the autonomic provision for their activity.
