RESUMO
A colored medium is described, consisting of dry nutrient agar, 1 percent mannose, and 0.02 percent bromothymol blue. Use of this medium helps accelerate the process of vibrio growth and differentiate mannose-positive vibrios from mannose-negative ones. The described medium is simple to make and use, thus recommending it for bacteriologic laboratories.
Assuntos
Meios de Cultura , Vibrio cholerae/isolamento & purificação , Técnicas BacteriológicasRESUMO
As the result of the serotyping of 1,223 NAG vibrio strains isolated in the Uzbek SSR from the environment and 659 strains isolated from gastroenteritis patients and from vibriocarriers, the serovars of 563 strains (46%) and 420 strains (63%), respectively, were determined. The prevailing serovars were 08, 06, 09, 014, 05, 039, 041, 013, 019 among the strains isolated from the environment and 08, 041, 037, 05, 039, 024, 06 among the strains isolated from humans. The strains belonging to serovars 05, 06, 08, 024, 037, 039, 041 were isolated both from the environment and from humans. The possible role of these strains in the development of acute gastroenteritis and in the formation of vibriocarriership in humans is pointed out.
Assuntos
Enteropatias/etiologia , Vibrioses/etiologia , Vibrio/isolamento & purificação , Doença Aguda , Portador Sadio/microbiologia , Gastroenterite/microbiologia , Humanos , Sorotipagem , Uzbequistão , Vibrio/classificação , Vibrio/patogenicidadeRESUMO
Elective-differentiating solid nutrient media for simultaneous isolation of Vibrioes, Salmonella and Shigella were developed. Antibiotics active against grampostive microflora and dry bile salts inhibiting the growth of Proteus were used as the inhibitors of the growth of the accompanying microflora. The medium was lincomycin and the bile salts may be prepared in a dry form.