Metabolic response of broiler chickens to different doses of Enterococcus faecium ICIS 96 in the diet.

The competitiveness and profitability of the poultry industry in market conditions are hampered by growing problems with the safety and quality of poultry meat. The use of targeted microbial preparations can assist in resolving these problems. Numerous studies of the properties of bacteria of the genus Enterococcus have shown their effectiveness in the practice of poultry farming. The objectives of this study were to identify the effect of various doses of Enterococcus faecium ICIS 96 added to the diet of broiler chickens on their metabolism and to evaluate the productive qualities of the chickens when different doses are used. The experiment was carried out on 72 Cobb-500 cross broiler chickens, divided into 3 groups. Chickens in the first group received a suspension of E. faecium ICIS 96 containing 1 × 108 cells in 1 mL of sterile saline, at a dose of 0.1 mL per 1 kg of live weight. The second group of chickens received a double dose of enterococcus, that is 0.2 mL of the suspension per 1 kg of live weight per day. The results demonstrated that 0.1 mL of a 1 × 108 per mL enterococcus culture suspension in the poultry diet per 1 kg of live weight per day intensified metabolism and increased the live weight of chickens after 40 d of treatment.

La compétitivité et la rentabilité de l'industrie de la volaille dans les conditions du marché sont désavantagées par les problèmes croissants de sécurité et de qualité de la viande de volaille. La solution à ce problème peut être trouvée grâce à l'utilisation de préparations microbiennes ciblées. De nombreuses études sur les propriétés des bactéries du genre Enterococcus suggèrent leur efficacité dans la pratique de l'aviculture. Le but de l'étude est d'évaluer l'effet de différentes doses d'E. faecium ICIS 96 sur le métabolisme et les qualités productives des poulets à griller. L'expérience a été réalisée sur 72 poulets de chair croisés Cobb-500, répartis en 3 groupes. Les poulets du premier groupe expérimental ont reçu une suspension de culture E. faecium ICIS 96, préparée à raison de 1 milliard de cellules microbiennes dans 1 mL de solution saline. Les poulets de chair du deuxième groupe expérimental ont reçu une dose doublée d'entérocoques, soit 0,2 mL d'une suspension de 1 milliard de cellules microbiennes pour 1 kg de poids vif par jour. Les résultats ont démontré que 0,1 mL d'une suspension de culture d'entérocoques dans l'alimentation des volailles pour 1 kg de poids vif par jour intensifiait le métabolisme et, par conséquent, augmentait le poids vif des poulets dans les 40 jours suivant l'ontogenèse post-incubation.(Traduit par Docteur Serge Messier).

Development of DNA aptamers for visualization of glial brain tumors and detection of circulating tumor cells.

Here, we present DNA aptamers capable of specific binding to glial tumor cells in vitro, ex vivo, and in vivo for visualization diagnostics of central nervous system tumors. We selected the aptamers binding specifically to the postoperative human glial primary tumors and not to the healthy brain cells and meningioma, using a modified process of systematic evolution of ligands by exponential enrichment to cells; sequenced and analyzed ssDNA pools using bioinformatic tools and identified the best aptamers by their binding abilities; determined three-dimensional structures of lead aptamers (Gli-55 and Gli-233) with small-angle X-ray scattering and molecular modeling; isolated and identified molecular target proteins of the aptamers by mass spectrometry; the potential binding sites of Gli-233 to the target protein and the role of post-translational modifications were verified by molecular dynamics simulations. The anti-glioma aptamers Gli-233 and Gli-55 were used to detect circulating tumor cells in liquid biopsies. These aptamers were used for in situ, ex vivo tissue staining, histopathological analyses, and fluorescence-guided tumor and PET/CT tumor visualization in mice with xenotransplanted human astrocytoma. The aptamers did not show in vivo toxicity in the preclinical animal study. This study demonstrates the potential applications of aptamers for precise diagnostics and fluorescence-guided surgery of brain tumors.

Genome Sequence of Enterococcus faecium Strain ICIS 96 Demonstrating Intermicrobial Antagonism Associated with Bacteriocin Production.

We report here the complete genome sequence of Enterococcus faecium strain ICIS 96, which was isolated from the feces of a horse. Bacteriological characterization of strain ICIS 96 revealed the absence of pathogenicity factors, while its spectrum of antagonistic activity was found to be broad, having activities associated with both Gram-positive and Gram-negative bacteria. Analysis of the E. faecium ICIS 96 genome revealed five genes associated with antimicrobial activity (enterocin [ent] A, ent B, lactobin A/cerein 7b, and ent L50 A/B). No genes that correlate with human pathogenicity were identified.