HISTOLOGICAL AND MORPHOLOGICAL CHANGES IN THE LYMPHOID STRUCTURES OF THE GASTRIC MUCOUS MEMBRANE IN WHITE RATS WITH THE ADMINISTRATION OF SODIUM GLUTAMATE.

OBJECTIVE: The aim: To determine the histological and morphological changes of the lymphoid structures of the stomach in male rats under the influence of oral sodium glutamate at the rate of 15 mg/kg of body weight. PATIENTS AND METHODS: Materials and methods: The scientific experiment was performed on 20 white non-linear male rats of reproductive age (4-5 months). The experimental animals were divided into two groups (10 rats in each group), which were orally received monosodium glutamate at a dose of 15 mg/kg body weight every day. We studied the effect of 2 and 4 weekly administration of monosodium glutamate at a dose of 15 mg/kg body weight, respectively, in the I and II groups of experimental animals (depending on the week of their decapitation). Rats of the control groups (n=10) were injected with a placebo for 2 and 4 weeks, namely 0.5 ml of dechlorinated tap water at room temperature. Intact control animals were also divided into two groups, 5 rats each, depending on the week of decapitation: respectively, III group - decapitation on the 2nd week of the experiment; IV group - decapitation on the 4th week of the experiment. After the experiments were completed, animals were decapitated under light ether anesthesia. According to the purpose of the study, pieces of rat stomach measuring 1.0 x 1.0 cm were taken from the front wall of the bottom of the stomach near the great curvature, cardiac and portal parts of the organ. Histological preparations were examined using a MICROmed SEO SСAN light microscope and a Vision CCD Camera. Morphometric studies were carried out according to the method of S. B. Stefanov, using grids No. 3/16. For electron microscopic examination, pieces of the stomach wall of rats were fixed in a 2.5% solution of glutaraldehyde in a 0.1 M phosphate buffer (pH 7.2-7.4) with subsequent fixation in a 2.0% solution of osmium tetroxide. After dehydration in alcohols and acetone, the material was embedded in eponaraldite. Sections were made on an LKB-8800-III ultramicrotome and studied using a JEM - 100-V microscope. To study the structural components of the lymphoid formations of the mucous membrane of different parts of the stomach of rats, semi-thin sections were made for the purpose of sharpening the blocks, which were stained with methylene blue. RESULTS: Results: The analysis of the obtained data of the conducted experiment indicates that the administration of monosodium glutamate in a dose of 15 mg/kg of body weight to rats already after 14 days leads to an increase in the density and size of the lymphoid structures of the GMM. The number of immunocompetent cells between the fundus of the gastric glands and the muscle plate increases in the diffuse lymphoid tissue of the gastrointestinal tract of rats in all its parts, both in the I and II groups of experimental animals. These changes are most pronounced in the cardiac and portal parts of the stomach. In both groups of experimental animals, the migration of interepithelial lymphocytes, macrophages, plasma cells, and tissue basophils to the surface epithelium increases. In both groups of experimental animals (and the II group of rats), lymphoid nodules and lymphoid pre-nodules of the gastric mucous membrane (GMM) are located between the bottom of the gastric glands and the muscular plate of the GMM. A gradual increase of medium lymphocytes in the GMM was established both in animals of I and II groups, while large lymphocytes increased in almost the same amount in experimental animals of both groups. Similar changes occur in the characteristics of the number of plasma cells, macrophages and tissue basophils in the lymphoid pre-nodules of GMM. CONCLUSION: Conclusions: Administering monosodium glutamate to rats at a dose of 15 mg/kg of body weight for 2 weeks leads to an increase in the density and size of lymphoid structures of the mucous membrane in all parts of the stomach with a predominant increase in the number of immunocompetent cells between the bottom of the gastric glands and the muscle plate. At the same time, more pronounced changes were found in the number of small lymphocytes, which tend to decrease by the 2nd week of the experiment, and vice versa - their density increases by the 4th week of monosodium glutamate administration.

HISTOLOGICAL AND MORPHOLOGICAL CHANGES OF THE VASCULAR BED OF THE THYMUS IN WHITE RATS UNDER THE INFLUENCE OF MONOSODIUM GLUTAMATE.

OBJECTIVE: The aim: To evaluate the effect of 28-day oral administration of MSG at the rate of 30 mg/kg of body weight on histological and morphometric parameters of the vascular bed of the thymus in rats. PATIENTS AND METHODS: Materials and methods: The scientific experiment was conducted on 20 white non-linear rats of reproductive age (4-5 months) weighing from 220 to 280 g, which were divided into two groups (10 rats each). Depending on the term of decapitation, the experimental animals were divided into two groups (10 rats in each group). We studied the effect of 14 and 28 days of MSG administration on the body of rats (I and II groups of experimental rats). The experimental animals were daily orally treated with MSG at a dose of 30 mg/kg body weight, which was dissolved in 0.5 ml of dechlorinated tap water at room temperature. Control rats of III and IV groups (5 rats in each of the control groups) were injected with a placebo (0.5 ml of dechlorinated tap water at room temperature) for 14 and 28 days. Intact animals of III and IV groups were also decapitated on the 14th and 28th days of the experiment, respectively. After the end of the experiment, animals were decapitated under light ether anesthesia. After decapitation, the animals were dissected into the chest cavity to remove the thymus. Histological preparations were studied using a MICROmed SEO SСAN light microscope and a Vision CCD Camera. Morphometric studies were carried out using VideoTest-5.0, KAARA Image Base and Microsoft Excel programs on a personal computer. RESULTS: Results: During the microscopic examination of histological preparations of the retrosternal gland in experimental animals of the 1st group (daily administration of MSG at the rate of 30 mg/kg of body weight for 14 days), it was established that the lumen of the arteries is moderately filled with blood elements. The veins are dilated with a changed shape and filled with blood. The following ultrastructural changes were detected in the experimental animals of group I: the lumen of arteries, arterioles and venules is slightly expanded, the nuclei of endotheliocytes are enlarged, occupy a significant part of the cytoplasm, the karyolem forms intussusceptions. The plasmolemma of the lumenal surface of endotheliocytes forms numerous microvilli. At the same time, organelles in the cytoplasm of endotheliocytes lose their contours. After 28 days of exposure to MSG at a dose of 30 mg/kg of body weight in rats (II group of experimental animals), structural changes in the vascular bed of the thymus worsened. The wall of arteries and arterioles is more thickened and swollen, collagen fibers are stratified. In their lumen, there are many uniform elements attached to the vascular wall and testify to thrombus formation. Perivascular edema is determined. The diameter of hemocapillaries is increased, their basal membrane is swollen. Veins and venules are also dilated, full blood, interendothelial contacts in the vessel wall are dilated, the basement membrane is damaged. This contributes to the diapedesis of blood plasma through the vessel wall, which leads to perivascular edema. CONCLUSION: Conclusions: Administration of MGS to rats at a dose of 30 mg/kg of body weight for 14 days leads to violations of the morphometric indicators of the vascular bed in the thymus, namely, to an increase in the outer and inner diameter of the arteries, an increase in the area of the middle membrane and the lumen of the vessels, which tend to progress with maximum indicators on the 28th day of the experiment. 2. The study of the vascular bed of the thymus against the background of taking MSG in a dose of 30 mg/kg of the weight of rats indicates the most pronounced changes in hemocapillaries, mainly on the 28th day of the experiment, which is manifested by an increase in their outer diameter. In the lumen of the hemocapillaries, deformed erythrocytes are identified, arranged in the type of "coin columns".

DEVELOPMENT AND FORMATION OF THE TOPOGRAPHY OF THE INFERIOR VENA CAVA AND PULMONARY VEINS DURING THE EIGHTH MONTH OF PRENATAL HUMAN ONTOGENESIS.

OBJECTIVE: The aim: To determine the topographic features and morphometric parameters of the pulmonary veins and inferior vena cava in human fetuses during the eighth month of intrauterine development. PATIENTS AND METHODS: Materials and methods: A morphometric and histological research of 25 human fetuses in the eighth month of prenatal ontogenesis (271.0 - 310.0 mm parietal-coccygeal length) was conducted. The topographic features of the inferior vena cava and pulmonary veins during the eighth month of intrauterine development were established, and their length and diameter, as well as the change in the angle of their formation, were morphometrically determined. The formation of the topography of these veins is due to the formation of the chest and abdominal organs and continues throughout the entire fetal period of development. RESULTS: Results: The inferior vena cava is formed at the level of the body of the IV lumbar vertebra in the eighth month of the intrauterine period of human development. The initial part of the trunk of the inferior vena cava obliquely "crosses" the common iliac artery from the left to the right. At this level, the vein is located to the right and slightly behind the aorta. In fetuses of the given age group, the right adrenal gland borders the inferior vena cava only at the level of the lower 2/3 of its medial edge. At the level of the subhepatic section of the inferior vena cava, 4-5 pairs of lumbar veins flow into it. In most cases, the ductus venosus flowed into the inferior vena cava independently between the middle and left hepatic veins. Compared to other sections of the inferior vena cava, the lumen of its diaphragmatic section, which passes through the opening in the dorsal part of the tendinous center of the diaphragm on the right, increases. During this period, it was established that the pulmonary veins lie completely in the heart cavity and are surrounded by a serous membrane. In all fetuses, two pulmonary veins flow from each lung into the left atrium. The level of confluence of the venous ducts of the pulmonary veins and their number varies individually. Pulmonary veins and their ducts lie more superficially and do not repeat the passage of the corresponding bronchi and arteries, lying, as a rule, within the limits of interlobular, intersegmental, and intersubsegmental connective tissue membranes. CONCLUSION: Conclusions: In this age group, changes in venous vessels and complications in the structure of their walls and their ducts continue. The passage and branching of the bronchial tree and arterial branches (starting with the segmental branches) are almost completely identical. A significant increase in the number of small ducts was found in the system of pulmonary veins. There is an increase in the morphometric indicators of the size of the inferior vena cava in comparison with its other parts. In the wall of the pulmonary veins and inferior vena cava, all three tunics are clearly visible, which in structure are close to the definitive one.