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Sci Rep ; 7: 46150, 2017 04 18.
Artigo em Inglês | MEDLINE | ID: mdl-28418034

RESUMO

Some cell lines retain intrinsic phototransduction pathways to control the expression of light-regulated genes such as the circadian clock gene. Here we investigated the photosensitivity of a Fugu eye, a cell line established from the eye of Takifugu rubripes, to examine whether such a photosensitive nature is present. Microarray analysis identified 15 genes that showed blue light-dependent change at the transcript level. We investigated temporal profiles of the light-induced genes, as well as Cry and Per, under light-dark, constant light (LL), and constant dark (DD) conditions by quantitative RT-PCR. Transcript levels of Per1a and Per3 genes showed circadian rhythmic changes under both LL and DD conditions, while those of Cry genes were controlled by light. All genes examined, including DNA-damage response genes and photolyase genes, were upregulated not only by blue light but also green and red light, implying the contribution of multiple photopigments. The present study is the first to identify a photosensitive clock cell line originating from a marine fish. These findings may help to characterize the molecular mechanisms underlying photic synchronization of the physiological states of fishes to not only daily light-dark cycles but also to various marine environmental cycles such as the lunar or semi-lunar cycle.


Assuntos
Ritmo Circadiano/genética , Ritmo Circadiano/efeitos da radiação , Olho/citologia , Regulação da Expressão Gênica/efeitos da radiação , Luz , Takifugu/genética , Takifugu/fisiologia , Análise de Variância , Animais , Proteínas CLOCK/genética , Proteínas CLOCK/metabolismo , Temperatura Alta , Modelos Biológicos , Análise de Sequência com Séries de Oligonucleotídeos , Filogenia , RNA Mensageiro/genética , RNA Mensageiro/metabolismo , Fatores de Tempo , Regulação para Cima/genética , Regulação para Cima/efeitos da radiação
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