Migration of hazardous contaminants from WEEE contaminated polymeric toy material by mouthing.

This study evaluated the migration of brominated flame retardants (BFRs), phosphate flame retardants (PFRs), bisphenols (BPA, BPF), and phthalate ester-based plasticizers from recycled polymeric toy material, containing waste electrical and electronic equipment (WEEE), in artificial saliva simulating 1 h of mouthing. In total 12 parts of 9 different toys were tested in triplicate after confirming WEEE specific contamination. Up to 11 contaminants were detected in saliva from one toy sample. The highest migration rate up to 128 ng/(cm2 x h) was found for BPA followed by bis(2-ethylhexyl) phthalate (DEHP) and diisobutyl phthalate (DIBP) with migration rates up to 25.5 and 8.27 ng/(cm2 x h), respectively. In addition to DecaBDE, which was detected in 3 saliva samples at migration rates between 0.09 and 0.31 ng/(cm2 x h), the decaBDE replacements 2,4,6-tris(2,4,6-tribromophenoxy)-1,3,5-triazine (TTBP-TAZ), decabromodiphenyl ethane (DBDPE), resorcinol bis(diphenyl phosphate) (RDP), and hexabromocyclododecane (HBCDD) were detected as well with comparable migration rates. 2,4,6-tribromphenol (246-TBP) reached migration rates up to 1.15 ng/(cm2 x h) in correspondence to the presence of TTBP-TAZ. Tetrabromobisphenol A (TBBPA), BPA, 246-TBP, DEHP, DIBP and triphenyl phosphate (TPHP) were predominantly observed in saliva with a detection frequency between 50 and 75%. Daily intake (DI) values were calculated for relevant analytes and compared to tolerable daily intake (TDI) values. The highest DI values of 72.4, 14.3, 5.74, 2.28 and 2.09 ng/(kg BW x day), were obtained for BPA, DEHP, DIBP, TBBPA, and TPHP, respectively. None of them exceed the TDI value or respective reference dose (RfD).

Multigeneration toxicity of imidacloprid and thiacloprid to Folsomia candida.

In a recent study, we showed that the springtail Folsomia candida was quite sensitive the neonicotinoid insecticides imidacloprid and thiacloprid. This study aimed at determining the toxicity of both compounds to F. candida following exposure over three generations, in natural LUFA 2.2 standard soil. In the first generation, imidacloprid was more toxic than thiacloprid, with LC50s of 0.44 and 9.0 mg/kg dry soil, respectively and EC50s of 0.29 and 1.5 mg/kg dry soil, respectively. The higher LC50/EC50 ratio suggests that thiacloprid has more effects on reproduction, while imidacloprid shows lethal toxicity to the springtails. In the multigeneration tests, using soil spiked at the start of the first generation exposures, imidacloprid had a consistent effect on survival and reproduction in all three generations, with LC50s and EC50s of 0.21-0.44 and 0.12-0.29 mg/kg dry soil, respectively, while thiacloprid-exposed animals showed clear recovery in the second and third generations (LC50 and EC50 > 3.33 mg/kg dry soil). The latter finding is in agreement with the persistence of imidacloprid and the fast degradation of thiacloprid in the test soil.