[A sensitive method for detection of sympathomimetic amines in pentafluorobenzamide form in biological samples using gas chromatography]. / Citlivý prukaz sympatomimetických aminu v biologických vzorcích ve forme pentafluorobenzamidu pomocí plynové chromatografie.

A toxicological method of sensitive and specific confirmation of methamphetamine and other primary and secondary amines in biological samples after extractive perfluorobenzoylation is described. The method is based on the principle of gas chromatography with parallel specific nitrogen detection and electron capture detection. The other alternative is the gas chromatography combined with mass spectrometry in chemical ionization mode. The method described allows the detection of methamphetamine and amphetamine in urine in concentrations below 10 ng/ml.

Gas chromatography of simple phenols in biological fluids.

Acid hydrolysis of phenol conjugates in urine by concentrated H3PO4 followed by extraction of phenols with n-hexane and their acetylation before gas chromatography on columns packed with OV-1 or OV-17 is described. The sensitivity of the method is sufficient to monitor normal levels of phenol and p-cresol or phenol and o-cresol after exposure to benzene or toluene vapours. The detection limit is 1 mg/l. The method can also be used to diagnose acute oral intoxication by phenol or cresols and to estimate its significance in clinical or forensic toxicology. Normal urine levels of phenols and those in different cases of human intoxication are evaluated.

Chlorinated phenols as metabolites of lindane. Evaluation of the degree of conjugation in rat urine.

After lindane was administered to rats in a single oral dose, the time profile of the degree of conjugation of the main phenolic metabolites was evaluated. In all urine samples 2,3-dichlorophenol, 2,4,5- and 2,4,6-trichlorophenol, and 2,3,5,6-tetrachlorophenol were constantly present. 2,3,5,6-Tetrachlorophenol and then 2,4,6-trichlorophenol were the prevalent metabolites in all cases. The degree of conjugation did not correlate with the dissociation constant of individual chlorinated phenols. Phenol conjugation declined with time after administration.

Rapid determination of ethylene glycol at toxic levels in serum and urine.

The rapid gas chromatographic detection and determination of ethylene glycol in biological fluids is described. Phenylboronic acid in acetone was used for the esterification of glycol. The phenylboronates of ethylene glycol and 1,2-propylene glycol are not separated on a packed column of medium polarity (OV-17), but they can be separated on a non-polar column (OV-101). In both instances, 1,3-propylene glycol can be used as an internal standard. The method requires only 100 microliters of serum or urine and is suitable for trace analysis in an emergency toxicological laboratory. The utility of the method is demonstrated on two cases of human intoxication with ethylene glycol.

[Chronic prostatitis and Bechterew's disease]. / Prostatitis chronica und Morbus Bechterew.

A group of patients between 35 and 65 years old with chronic prostatitis were examined for the presence of Becherew's disease. In this connection the New York and Roman criterions for morbus Bechterew were applied. There were found one ankyosing spondylarthritis, one ankylosis of the sacroiliac joint, and 11 times a tentative sacroileitis were stated. Altogether the proved and tentative findings were only 3.68 per cent of all examinations. In our countries the morbus Bechterew is found in 0,21 per cent of the normal population. So the protion of the Bechterew's disease in patients with chronic prostatitis is indeed a little higher than average, but not so frequent as often pretended in recent times. After a second series 58 patients being treated because of Bechterew's disease of different stages and different terms were examined for the possibility of a simultaneously elapsing chronic prostatitis. A chronic prostatitis was found in 38 per cent of these patients which correspondents to the incidence published in literature for the medium-age manhood. Nobody of the test persons had complaints on the part of the urologenital tract.