Micrometric measurement of the density of stained odontoblast processes

The embryological, structural and functional unit of the dentine-pulp complex shares the odontoblast, located in the border of the dentine pulp, with basal nuclei and organelles. The odontoblast process emerges from its apical pole. It is formed by microtubules, microfilaments and vesicles covered by membranes penetrating the dentinal tubules, isolated from the inter-tubular matrix, along the extent of the dentine. The objective of this study was to evaluate the efficacy of three staining techniques: hematoxylin-eosin, periodic acid-Schiff and Schmorl, by staining the process, from beginning to end, and compare the results with the erosion technique. Thirty human teeth were employed in the trial; after their extraction the pulp was fixated, the pieces demineralized in nitric acid at 8%, the collagen filaments eliminated with Type II Collagenase, the tissue was stained, and the measurements were made. The portions with no pulp were prepared with the erosion technique. Results: Comparing the best results obtained by staining with the values obtained with the erosion technique, the former showed lower values. Conclusion: Staining techniques show lower density of the staining processes compared with the dentinal tubules in the erosion technique

Micrometric measurement of the density of stained odontoblast processes

The embryological, structural and functional unit of the dentine-pulp complex shares the odontoblast, located in the border of the dentine pulp, with basal nuclei and organelles. The odontoblast process emerges from its apical pole. It is formed by microtubules, microfilaments and vesicles covered by membranes penetrating the dentinal tubules, isolated from the inter-tubular matrix, along the extent of the dentine. The objective of this study was to evaluate the efficacy of three staining techniques: hematoxylin-eosin, periodic acid-Schiff and Schmorl, by staining the process, from beginning to end, and compare the results with the erosion technique. Thirty human teeth were employed in the trial; after their extraction the pulp was fixated, the pieces demineralized in nitric acid at 8%, the collagen filaments eliminated with Type II Collagenase, the tissue was stained, and the measurements were made. The portions with no pulp were prepared with the erosion technique. Results: Comparing the best results obtained by staining with the values obtained with the erosion technique, the former showed lower values. Conclusion: Staining techniques show lower density of the staining processes compared with the dentinal tubules in the erosion technique

Hábitos alimentarios y experiencia de caries en adultos jóvenes en Rosario Argentina / Food habits and caries experience in young adult from Rosario Argentina

En Rosario la atención odontológica se brinda en ámbitos privados y públicos. Los servicios públicos carecen de programas para evitar la pérdida dentaria en adultos. Nuestro objetivo fue describir el perfil epidemiológico de experiencia de caries y hábitos dietéticos en tres sub-poblaciones de adultos jóvenes. Aplicando una anamnesis estandarizada se investigaron 517 pacientes, ambos sexos, 15-45 años, concurrentes a un servicio privado (19 por ciento) y dos públicos (81 por ciento), uno céntrico y otro marginal. Variables investigadas: consumo de lácteos y alimentos dulces, estado bucal y escolaridad. Se aplicaron pruebas estadísticas t de student, ANOVA y chi-cuadrado. Promedios hallados: cariados 1,7 ±2,0 en privado, 4,1±2,8 en públicos (p=0.0001); ausentes 5,4±5,2 en privado, 6,8±5,4 en públicos (p=0.04); obturados 4,4±4,2 en privado, 1,3±2,2 en públicos (p<0.0001). Nivel de escolaridad: superior en el privado (p<0.0001). Consumo diario de lácteos: mayoría en privados (p<0.001). En los servicios públicos los pacientes reemplazan la leche por el mate y relacionan su ingesta con la gravidez, lactancia o la dejan para los niños. Tipos de golosinas o dulces de riesgo consumidos: en el sector privado fueron panificados tipo facturas, tortas, galletitas y dulces, y en los públicos caramelos blandos, alfajores y dulce de leche. El consumo de alimentos dulces de menor riesgo fue de helados, pastillas, gaseosas y chocolates en los privados y de caramelos duros y chicles con y sin azúcar en los públicos. Si bien un alto número de personas los ingieren a cualquier hora, los pacientes privados fueron minoría, mientras que en su consumo sólo después de una comida éstos fueron mayoría.

[Food habits and caries experience in young adults from Rosario, Argentina]. / Hábitos alimentarios y experiencia de caries en adultos jóvenes en Rosario, Argentina.

Odontological assistance in Rosary City is offered in private and public services. The public ones lack programs to avoid dental loss in adults. Our aim was to describe the caries experience and dietetic habits in three sub-populations of young adults. Applying a standardized anamnesis, 517 patients, both sexes, 15-45 years old, attending to a private service (19%) and two public ones (81%), were investigated. Studied variables: oral health, lacteal and sweet food consumed, and instruction level. Student t-test, ANOVA and chi-square tests were applied. Average decayed teeth were 1.7+/-2.0 in the private service, and 4.1+/-2.8 in the public ones (p<0.0001); missing teeth were 5.4+/-5.2 and 6.8+/-5.4 (p=0.04); and filled teeth were 4.4+/-4.2 and 1.3+/-2.2, respectively (p<0.0001). Dairy foods daily consumption was higher in the private service (p<0.001). Patients in the public services expressed to replace milk by mate, and to relate its consumption with pregnancy and lactation, or to leave it for children. Sweets delicacies consumed in the private sector -considered of risk- were sweet breads, cakes, cookies and sweets, and in the public ones were soft candies, "alfajores" and sweet made with milk. Minor risk sweets consumption in private patients were ice creams, sodas and chocolates, and in the public ones, hard candies and chewing gums, with and without sugar. Although a high number of patients consume delicacies and sweet foods at any hour, private patients were a minority; and they were a majority when considering its ingestion only after a food.