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1.
J Vet Intern Med ; 33(2): 846-855, 2019 Mar.
Artigo em Inglês | MEDLINE | ID: mdl-30666726

RESUMO

BACKGROUND: Increased incidence of bacterial pneumonia (BP) has been reported in Irish Wolfhounds (IWHs), and recurrence of BP is common. The etiology of recurrent pneumonia in IWHs is largely unknown. OBJECTIVES: To describe clinical findings in IWHs with recurrent BP and investigate possible etiologies. ANIMALS: Eleven affected IWHs, 25 healthy IWHs, 28 healthy dogs of other Sighthound breeds, and 16 healthy dogs of other breeds. METHODS: Prospective cross-sectional observational study. All affected IWHs underwent thorough clinical examinations including thoracic radiographs, thoracic computed tomography, electron microscopic evaluation of ciliary structure, and bronchoscopy and bronchoalveolar lavage fluid (BALF) cytology and culture. Serum and BALF immunoglobulin concentrations were measured using an ELISA method, and peripheral blood lymphocyte subpopulations were analyzed using flow cytometry. Esophageal function was assessed by fluoroscopy (n = 2). RESULTS: Median age of onset was 5.0 years (range, 0.4-6.5 years), and when presented for study, dogs had experienced a median of 5 previous episodes of BP (range, 2-6). The following predisposing factors to BP were detected: focal bronchiectasis (10/11), unilateral (2/9) and bilateral (1/9) laryngeal paralysis, and esophageal hypomotility (2/2). Local or systemic immunoglobulin deficiencies or primary ciliary defects were not detected. CONCLUSIONS AND CLINICAL IMPORTANCE: Recurrent BP affects mostly middle-aged and older IWHs without any evident immune deficit or primary ciliary defects. Focal BE was a frequent finding in affected dogs and likely contributed to the development of recurrent respiratory infections. Laryngeal and esophageal dysfunction identified in a minority of dogs may contribute to recurrent BP.


Assuntos
Líquido da Lavagem Broncoalveolar/imunologia , Doenças do Cão/microbiologia , Pneumonia Bacteriana/veterinária , Animais , Bronquiectasia/veterinária , Líquido da Lavagem Broncoalveolar/citologia , Líquido da Lavagem Broncoalveolar/microbiologia , Estudos Transversais , Doenças do Cão/diagnóstico por imagem , Doenças do Cão/etiologia , Cães , Transtornos da Motilidade Esofágica/diagnóstico por imagem , Transtornos da Motilidade Esofágica/veterinária , Feminino , Imunoglobulinas/análise , Imunoglobulinas/sangue , Subpopulações de Linfócitos , Masculino , Pneumonia Bacteriana/diagnóstico por imagem , Pneumonia Bacteriana/etiologia , Estudos Prospectivos , Radiografia Torácica/veterinária , Tomografia Computadorizada por Raios X/veterinária , Paralisia das Pregas Vocais/veterinária
2.
Am J Vet Res ; 78(11): 1329-1337, 2017 Nov.
Artigo em Inglês | MEDLINE | ID: mdl-29076374

RESUMO

OBJECTIVE To examine whether expression of extracellular matrix metalloproteinase inducer (EMMPRIN) can be detected in equine lungs and whether it correlates with matrix metalloproteinase (MMP)-2 and -9 expression in bronchoalveolar lavage fluid (BALF) of horses with chronic inflammation of the lungs (ie, lower airway inflammation [LAI]). ANIMALS 29 horses with signs of chronic respiratory tract disease, which were classified as the LAI (n = 17) and LAI with respiratory distress (RDLAI [12]) groups, and 15 control horses. PROCEDURES BALF, tracheal aspirate, and blood samples were obtained, and EMMPRIN expression was determined from BALF cells and RBCs by use of western blotting. Activities of MMP-2 and -9 were determined with zymography. RESULTS Expression of EMMPRIN protein was identified in BALF cells of all horses. Expression of EMMPRIN protein was highest for the RDLAI group and was correlated with MMP-2 and -9 protein expression, MMP-9 gelatinolytic activity, and airway neutrophilia. CONCLUSIONS AND CLINICAL RELEVANCE Results suggested that EMMPRIN was involved in the pathophysiologic processes of asthma in horses. However, additional studies of horses and other species are warranted to elucidate the regulation of EMMPRIN expression in asthmatic lungs.


Assuntos
Basigina/biossíntese , Doenças dos Cavalos/enzimologia , Metaloproteinase 2 da Matriz/biossíntese , Metaloproteinase 9 da Matriz/biossíntese , Doenças Respiratórias/veterinária , Animais , Basigina/metabolismo , Western Blotting/veterinária , Líquido da Lavagem Broncoalveolar , Doença Crônica , Feminino , Doenças dos Cavalos/metabolismo , Cavalos , Inflamação , Pulmão/enzimologia , Pulmão/metabolismo , Masculino , Doenças Respiratórias/enzimologia , Doenças Respiratórias/metabolismo
3.
Am J Vet Res ; 76(2): 161-9, 2015 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-25629914

RESUMO

OBJECTIVE: To characterize the expression of monocarboxylate transporters (MCTs) 1 and 4 and the ancillary protein CD147 in the intestinal tract of healthy equids and determine the cellular location of CD147 in the intestinal epithelium. ANIMALS: 12 healthy horses and ponies slaughtered for meat production or euthanized for reasons unrelated to gastrointestinal tract disease. PROCEDURES: The entire gastrointestinal tract was removed from each equid within 45 minutes after slaughter or euthanasia. Tissue samples were obtained from the antimesenteric side of the duodenum, jejunum, ileum, middle part of the cecum, sternal flexure of the ventral colon, pelvic flexure, sternal flexure of the dorsal colon, and descending colon (small colon). Expressions of MCT1, MCT4, and the ancillary protein CD147 were examined in tissue samples from each of the 8 intestinal locations by means of quantitative PCR assay, immunoblotting, and immunohistochemical analyses. RESULTS: Expression of MCT1 was most abundant in the cecum and colonic sites, whereas expression of MCT4 was predominantly in the proximal section of the intestine (small intestinal sites and cecum). Immunohistochemical analysis revealed that MCT1 and CD147 were present in the membranes of enterocytes (in crypts and villi). CONCLUSIONS AND CLINICAL RELEVANCE: The anatomic distribution of MCT1 and MCT4 in the equine intestinal tract determined in this study together with the previous knowledge of the sites of substrate absorption indicated that MCT1 might predominantly contribute to the uptake of short-chain fatty acids in the large intestine and MCT4 might predominantly contribute to the uptake of lactate in the small intestine.


Assuntos
Basigina/metabolismo , Cavalos/metabolismo , Mucosa Intestinal/metabolismo , Transportadores de Ácidos Monocarboxílicos/metabolismo , Animais , Feminino , Imuno-Histoquímica/veterinária , Absorção Intestinal , Masculino
4.
Vet J ; 198(1): 122-6, 2013 Oct.
Artigo em Inglês | MEDLINE | ID: mdl-23838207

RESUMO

The reason why some horses begin an oral stereotypy such as crib-biting is not known. The aim of this study was to measure ghrelin and leptin concentrations in plasma concentrations to determine whether there is a link to crib-biting in horses. Plasma samples (n=3) were collected for plasma leptin and ghrelin assay before and during the morning first feeding in the usual environments of 15 horses with stereotypic crib-biting and 15 matched controls. The crib-biting intensity was scored in three 5-min phases, and a subgroup of verified crib-biters (n=8) was defined as horses that were seen to crib-bite during this study. Plasma leptin concentration (mean and 95% confidence interval [CI]) was lower in horses observed to crib-bite before and after feeding of concentrates (1.2, CI 0.8-1.7 ng/mL and 1.0, CI 0.6-1.7) than in non-crib-biters (2.3, CI 1.6-3.4 and 2.3, CI 1.6-3.4 ng/mL, respectively) and correlated negatively with crib-biting intensity. Crib-biting intensity was significantly higher shortly after feeding than before or 30 min later. Plasma ghrelin concentration was significantly higher before feeding concentrate than before hay feeding or after the concentrate, but did not differ between groups. There was a significant negative correlation between body composition score and plasma ghrelin concentration. These findings suggest that leptin concentrations may be associated with crib-biting behaviour in horses.


Assuntos
Ingestão de Alimentos , Grelina/sangue , Cavalos/fisiologia , Leptina/sangue , Comportamento Estereotipado , Fenômenos Fisiológicos da Nutrição Animal , Animais , Estudos de Casos e Controles , Feminino , Masculino , Estações do Ano
5.
Vet J ; 184(1): 100-4, 2010 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-19237301

RESUMO

A prospective observational study was performed to evaluate whether the plasma concentration of heat shock protein 72 (HSP72) or beta-endorphin is related to clinical signs, blood chemistry, or severity of pain of colic. Seventy-seven horses with colic and 15 clinically healthy controls were studied. The horses were divided into four groups which reflected increasing severity of colic, from normal control horses to horses with mild, moderate and severe colic. Blood samples were collected before any treatment. Packed cell volume (PCV) and plasma HSP72, beta-endorphin, cortisol, adrenocorticotropic hormone (ACTH) and lactate concentrations were measured. Plasma beta-endorphin was related with severity of colic and survival, as well as with plasma cortisol, ACTH and lactate concentrations, heart rate, PCV and pain score. High plasma HSP72 concentration may indicate circulatory deficits, but was not associated with clinical signs of colic. Plasma lactate still seemed to be the most useful single prognostic parameter in horses with colic.


Assuntos
Cólica/veterinária , Proteínas de Choque Térmico HSP72/sangue , Doenças dos Cavalos/sangue , beta-Endorfina/sangue , Hormônio Adrenocorticotrópico/sangue , Animais , Biomarcadores/sangue , Análise Química do Sangue/veterinária , Estudos de Casos e Controles , Cólica/sangue , Cólica/mortalidade , Cólica/patologia , Feminino , Doenças dos Cavalos/mortalidade , Doenças dos Cavalos/patologia , Cavalos , Hidrocortisona/sangue , Ácido Láctico/sangue , Masculino , Dor/sangue , Dor/veterinária , Prognóstico , Estudos Prospectivos , Índice de Gravidade de Doença
6.
Am J Vet Res ; 69(8): 1091-6, 2008 Aug.
Artigo em Inglês | MEDLINE | ID: mdl-18672976

RESUMO

OBJECTIVE: To detect monocarboxylate transporters (MCTs) in canine RBC membranes and to determine the distribution of lactate between plasma and RBCs. SAMPLE POPULATION: Blood samples obtained from 6 purpose-bred Beagles. PROCEDURES: Monocarboxylate transporter isoforms 1, 2, 4, 6, 7, and 8 and CD147 were evaluated in canine RBCs by use of western blot analysis. Lactate influx into RBCs was measured as incorporation of radioactive lactate. RESULTS: 2 MCT isoforms, MCT1 and MCT7, were detected in canine RBC membranes on western blot analysis, whereas anti-MCT2, anti-MCT4, anti-MCT6, and anti-MCT8 antibodies resulted in no signal. No correlation was found between the amount of MCT1 or MCT7 and lactate transport activity, but the ancillary protein CD147 that is needed for the activity of MCT1 had a positive linear correlation with the rate of lactate influx. The apparent Michael is constant for the lactate influx in canine RBCs was 8.8 +/- 0.9mM. Results of in vitro incubation studies revealed that at lactate concentrations of 5 to 15mM, equilibrium of lactate was rapidly obtained between plasma and RBCs. CONCLUSIONS AND CLINICAL RELEVANCE: These results indicated that at least half of the lactate transport in canine RBCs occurs via MCT1, whereas MCT7 may be responsible for the rest, although an additional transporter was not ruled out. For practical purposes, the rapid equilibration of lactate between plasma and RBCs indicated that blood lactate concentrations may be estimated from plasma lactate concentrations.


Assuntos
Eritrócitos/metabolismo , Lactatos/sangue , Animais , Transporte Biológico , Cães , Cinética , Lactatos/farmacocinética , Transportadores de Ácidos Monocarboxílicos/sangue , Transportadores de Ácidos Monocarboxílicos/metabolismo
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