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1.
Anticancer Res ; 30(5): 1653-9, 2010 May.
Artigo em Inglês | MEDLINE | ID: mdl-20592357

RESUMO

BACKGROUND: Malignant tumors metabolize glucose to lactate even in the presence of oxygen (aerobic glycolysis). The metabolic switch from oxidative glycolysis to non-oxidative fermentation of glucose and proteins performed by the tumor cells seems to be associated with TKTL1 and pAkt overexpression. Therefore the aim of the present study was to investigate the expression of TKTL1 and pAkt in human specimens of endometrial cancer as compared to benign endometrium. Additionally, expression of the glucose transporter GLUT1 was also investigated as aerobic glycolysis is associated with an increased need for glucose. MATERIALS AND METHODS: Levels of TKTL1, pAkt, and GLUT1 expression were immunhistochemically evaluated on paraffin embedded biopsy material from 10 benign and 41 malignant endometrial tissue samples. TKTL1 mRNA levels in the endometrial cancer cell lines Ishikawa and HEC-1A were evaluated by RT-PCR. RESULTS: Expression of TKTL1, GLUT1 and pAKT was significantly increased in endometrial carcinomas as compared to benign endometrial tissue. There was a significantly weaker TKTL1 expression in highly differentiated G1 tumors. In the human endometrial cancer cell lines Ishikawa and HEC-1A, TKTL1 mRNA was clearly detectable. CONCLUSION: The levels of TKTL1, GLUT1 and pAKT expression point to the glycolytic phenotype of malignant endometrial tissue. Given the pronounced TKTL1 expression across all different subtypes of endometrial cancer, this protein could serve as a target for future cancer treatments.


Assuntos
Neoplasias do Endométrio/metabolismo , Perfilação da Expressão Gênica , Regulação Neoplásica da Expressão Gênica , Transcetolase/biossíntese , Idoso , Linhagem Celular Tumoral , Feminino , Glucose/metabolismo , Transportador de Glucose Tipo 1/metabolismo , Glicólise , Humanos , Imuno-Histoquímica/métodos , Pessoa de Meia-Idade , Oxigênio/química , RNA Mensageiro/metabolismo
2.
Arch Gynecol Obstet ; 277(2): 175-8, 2008 Feb.
Artigo em Inglês | MEDLINE | ID: mdl-17674013

RESUMO

OBJECTIVE: Lymphoepithelioma-like carcinoma (LELC) is a rare variant of squamous cell carcinoma of the uterine cervix. In contrast to Asian women, Caucasian women have a low prevalence of cervical LELC with no infection of the Epstein-Barr virus (EBV) and sporadic appearances of human papilloma viruses (HPV) in Caucasian women. CASE: We report a 36-year-old female Caucasian patient with LELC of the cervix. RESULT: In contrast to the present studies, our case of LELC of the cervix is not associated with EBV or high-risk HPV appearances. CONCLUSION: This is the first study, that showed no relationship between LELC of the uterine cervix with EBV and high-risk HPV infections in a Caucasian woman.


Assuntos
Carcinoma de Células Escamosas/patologia , Neoplasias do Colo do Útero/patologia , Adulto , Carcinoma de Células Escamosas/cirurgia , Infecções por Vírus Epstein-Barr , Feminino , Humanos , Histerectomia , Excisão de Linfonodo , Metrorragia/etiologia , Infecções por Papillomavirus , Neoplasias do Colo do Útero/cirurgia , População Branca
3.
Int J Gynecol Cancer ; 16(6): 2018-22, 2006.
Artigo em Inglês | MEDLINE | ID: mdl-17177840

RESUMO

Galectin-1, a member of the beta-galactoside-binding family, is widely expressed in epithelial and immune cells. It is involved in several normal and pathologic processes, such as cancer progression, metastasis, and immunobiology. Galectin-1 was found to be overexpressed in various cancer cells and the corresponding benign tissue. Therefore, it has been described as a marker for tumor progression in some malignancies. In the current study, the expression of galectin-1 was examined in 80 formalin-fixed, paraffin-embedded cervical tissues: 20 benign cervical specimen, 20 low-grade squamous intraepithelial lesions (LGSIL), 20 high-grade squamous intraepithelial lesions (HGSIL), and 20 invasive squamous cell carcinomas (ISCC). Immunohistochemical analyses showed that the intensity of the galectin-1 expression on stromal cells next to the transformed cells increased according to the pathologic grade: benign cervical tissue < LGSIL < HGSIL < ISCC (P < 0.001). The epithelial cells were always negative for galectin-1. These results suggest that galectin-1 expression on stromal cells increases with the histopathologic grade of cervical tissues, and it can be concluded that this increase is associated with the progression of cervical neoplasia.


Assuntos
Galectina 1/metabolismo , Regulação Neoplásica da Expressão Gênica , Regulação para Cima , Neoplasias do Colo do Útero/metabolismo , Neoplasias do Colo do Útero/patologia , Progressão da Doença , Feminino , Humanos , Imuno-Histoquímica
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