RESUMO
The Beaudette 42 strain of avian infectious bronchitis virus, Sato strain of Newcastle disease virus, and Uchida strain of avian reovirus were passaged in Chinese hamster lung cells (Don), and some properties were examined. The Don-passaged strains showed a difference in replication in Don and chicken embryo kidney cells in one-step growth curve examinations and a partial modification in pathogenicity to chicken embryos; nevertheless, neutralization tests revealed no serological alteration.
Assuntos
Embrião de Galinha/microbiologia , Coronaviridae/patogenicidade , Vírus da Bronquite Infecciosa/patogenicidade , Vírus da Doença de Newcastle/patogenicidade , Reoviridae/patogenicidade , Animais , Linhagem Celular , Cricetinae , Vírus da Bronquite Infecciosa/crescimento & desenvolvimento , Rim , Vírus da Doença de Newcastle/crescimento & desenvolvimento , Reoviridae/crescimento & desenvolvimentoRESUMO
Eight etiologic agents isolated from field cases in Japan were identified as isolates of infectious bronchitis virus by agar-gel diffusion, buoyant-density determination on sucrose-density centrifugation, and morphological study by electron microscope of the purified viruses. In studies of the antigenic relationships of the eight isolates and six known infectious bronchitis viruses, antigenic diversity of these viruses was recognized from the degrees of relatedness using a plaque reduction in the "constant-virus decreasing-serum" method.
