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1.
Curr Eye Res ; 39(5): 512-7, 2014 May.
Artigo em Inglês | MEDLINE | ID: mdl-24400913

RESUMO

PURPOSE: To study the effect of acute exposure of rabbit eyes to artificial sunlight in vivo, on the integrity of corneal and conjunctival tissue as well as on the gene expression of the receptor for platelet activating factor (PAFR). METHODS: New Zealand albino rabbits were immobilized opposite a 300 W Osram Ultra-Vitalux® light bulb with an emission radiation spectrum similar to that of normal sunlight at noon, and exposed to ultraviolet B radiation in the range of the reported threshold for corneal damage. Corneal and third eyelid tissue samples were removed from exposed eyes at 2, 6 and 24 h following the end of the exposure to the bulb light and were subsequently processed for histochemical staining and RNA extraction. The gene expression of PAFR was detected with real time reverse transcription polymerase chain reaction. RESULTS: Some epithelial shedding was detected in the corneal tissue as a result of acute exposure to artificial sunlight. In the eyelid conjunctiva, a marked accumulation of eosinophils was noticed, as early as 2 h post-exposure, apparently directed toward the upper part of the epithelial layer. This effect appears to subside by hour 24. No statistically significant changes in gene expression were detected in the corneal tissue, whereas in the third eyelid, PAFR gene expression was significantly induced, most prominently at t = 2 and 6 h post-exposure. CONCLUSION: Acute exposure of rabbit eyes to artificial sunlight induced a marked infiltration of eosinophils into the epithelial layer of the conjunctiva but no gross alterations in the cornea or the third eyelid. The gene expression of PAFR was upregulated, as an effect of light exposure, in the third eyelid but not in the cornea.


Assuntos
Túnica Conjuntiva/efeitos da radiação , Córnea/efeitos da radiação , Iluminação/efeitos adversos , Membrana Nictitante/efeitos da radiação , Glicoproteínas da Membrana de Plaquetas/genética , Receptores Acoplados a Proteínas G/genética , Animais , Túnica Conjuntiva/patologia , Túnica Conjuntiva/fisiologia , Córnea/patologia , Córnea/fisiologia , Eosinófilos/patologia , Eosinófilos/efeitos da radiação , Expressão Gênica/efeitos da radiação , Iluminação/métodos , Membrana Nictitante/patologia , Membrana Nictitante/fisiologia , Estimulação Luminosa/métodos , Coelhos , Luz Solar
2.
Free Radic Res ; 43(4): 385-9, 2009 Apr.
Artigo em Inglês | MEDLINE | ID: mdl-19274593

RESUMO

This study attempted to examine the effect of a functional catalase gene polymorphism, CAT -262C>T, on sodium-lithium countertransport (Na-Li CT) activity, insulin resistance determined as the homeostasis model assessment index (HOMA-IR), blood lipid parameters (cholesterol, triglycerides, low density lipoprotein cholesterol, high density lipoprotein cholesterol, apolipoprotein B, apolipoprotein A-I) and their response to atorvastatin, in previously characterized Greek dyslipidaemic patients and normolipidaemic controls. Putative associations were examined by running univariate analyses with a general linear model, using age, sex, smoking and hypertension as covariates. While no statistically significant associations were detected between the CAT -262C>T polymorphism and either baseline values or their modulation by atorvastatin in the patient group, HOMA-IR values were significantly (p=0.028) lower among CAT -262CC controls compared to their T allele carrier counterparts. A trend towards higher plasma triglyceride values among CAT -262CC genotypes was also detected, in both dyslipidaemic patients and normolipidaemic controls.


Assuntos
Antiporters/sangue , Catalase/genética , Dislipidemias/sangue , Dislipidemias/genética , Ácidos Heptanoicos/farmacologia , Resistência à Insulina/genética , Lipídeos/sangue , Pirróis/farmacologia , Adulto , Alelos , Atorvastatina , Estudos de Casos e Controles , Dislipidemias/tratamento farmacológico , Eritrócitos/efeitos dos fármacos , Eritrócitos/metabolismo , Feminino , Grécia , Humanos , Inibidores de Hidroximetilglutaril-CoA Redutases/farmacologia , Modelos Lineares , Masculino , Pessoa de Meia-Idade , Polimorfismo de Nucleotídeo Único , Estudos Retrospectivos
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