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1.
Polymers (Basel) ; 13(17)2021 Sep 04.
Artigo em Inglês | MEDLINE | ID: mdl-34503043

RESUMO

We report on a new approach toward a laser-assisted modification of biocompatible polydimethylsiloxane (PDMS) elastomers relevant to the fabrication of stretchable multielectrode arrays (MEAs) devices for neural interfacing technologies. These applications require high-density electrode packaging to provide a high-resolution integrating system for neural stimulation and/or recording. Medical grade PDMS elastomers are highly flexible with low Young's modulus < 1 MPa, which are similar to soft tissue (nerve, brain, muscles) among the other known biopolymers, and can easily adjust to the soft tissue curvatures. This property ensures tight contact between the electrodes and tissue and promotes intensive development of PDMS-based MEAs interfacing devices in the basic neuroscience, neural prosthetics, and hybrid bionic systems, connecting the human nervous system with electronic or robotic prostheses for restoring and treating neurological diseases. By using the UV harmonics 266 and 355 nm of Nd:YAG laser medical grade PDMS elastomer is modified by ns-laser ablation in water. A new approach of processing is proposed to (i) activate the surface and to obtain tracks with (ii) symmetric U-shaped profiles and (iii) homogeneous microstructure This technology provides miniaturization of the device and successful functionalization by electroless metallization of the tracks with platinum (Pt) without preliminary sensitization by tin (Sn) and chemical activation by palladium (Pd). As a result, platinum black layers with a cauliflower-like structure with low values of sheet resistance between 1 and 8 Ω/sq are obtained.

2.
Int Microbiol ; 14(3): 163-71, 2011 Sep.
Artigo em Inglês | MEDLINE | ID: mdl-22101414

RESUMO

Laboratory scale continuous-flow-through chambers (flow cells) facilitate the observation of microbes in a controlled, fully hydrated environment, although these systems often do not simulate the environmental conditions under which microorganisms are found. We developed a flow cell that mimics a subsurface groundwater-saturated rock fracture and is amenable to confocal laser scanning microscopy while allowing for the simple removal of the attached biomass. This flow cell was used to investigate the effect of toluene, a representative contaminant for non-aqueous phase liquids, on groundwater-derived biofilms. Reduced average biofilm biomass and thickness, and diminished diversity of amplifiable 16S rRNA sequences were observed for biofilms that developed in the presence of toluene, compared to the biofilms grown in the absence of toluene. The flow cell also allowed the detection of fluorescent protein-labelled cells.


Assuntos
Biofilmes , Citometria de Fluxo/métodos , Água Subterrânea/microbiologia , Pseudomonas putida/genética , Tolueno/efeitos adversos , Microbiologia da Água , Biofilmes/efeitos dos fármacos , Conjugação Genética , Eletroforese em Gel de Gradiente Desnaturante , Citometria de Fluxo/instrumentação , Corantes Fluorescentes/análise , Genes Reporter , Proteínas de Fluorescência Verde/análise , Consórcios Microbianos/efeitos dos fármacos , Microscopia Confocal/métodos , Reação em Cadeia da Polimerase , Pseudomonas putida/metabolismo , Poluentes Químicos da Água/efeitos adversos
3.
Int. microbiol ; 14(3): 163-171, sept. 2011. ilus
Artigo em Inglês | IBECS | ID: ibc-98739

RESUMO

Laboratory scale continuous-flow-through chambers (flow cells) facilitate the observation of microbes in a controlled, fully hydrated environment, although these systems often do not simulate the environmental conditions under which microorganisms are found. We developed a flow cell that mimics a subsurface groundwater-saturated rock fracture and is amenable to confocal laser scanning microscopy while allowing for the simple removal of the attached biomass. This flow cell was used to investigate the effect of toluene, a representative contaminant for non-aqueous phase liquids, on groundwater-derived biofilms. Reduced average biofilm biomass and thickness, and diminished diversity of amplifiable 16S rRNA sequences were observed for biofilms that developed in the presence of toluene, compared to the biofilms grown in the absence of toluene. The flow cell also allowed the detection of fluorescent protein-labelled cells (AU)


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Assuntos
Biofilmes/crescimento & desenvolvimento , Água Subterrânea/microbiologia , Microscopia Confocal/métodos , Adesinas Bacterianas/análise , Poluição de Águas Subterrâneas/análise , Pseudomonas putida/crescimento & desenvolvimento , Microscopia de Fluorescência
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