[Cellular immunity in patients with non-specific inflammatory bowel disease for an extended period after surgery]. / Odpornosc komórkowa u chorych z nieswoistymi chorobami zapalnymi jelit w odleglym czasie po operacji.

The study was aimed at investigating the expression of HLA-DR, CD4, CD8, CD56 surface antigens on peripheral blood lymphocytes in patients with non-specific inflammatory bowel diseases in the period of 1-5 years after the surgical procedure. The percentage of rosette forming T-cells in the theophylline test and skin reaction to mycotic and bacterial antigens were assessed simultaneously. The experiment included group of 12 patients (aged 5-55), treated surgically because of Crohn's disease or ulcerative colitis. The lymphocytes' surface antigens were examined by means of monoclonal antibodies and the APAAP procedure. Significantly increased (p < 0.001) percentage of lymphocytes with HLA-DR surface antigens and significantly decreased (p < 0.002) percentage of lymphocytes with CD8 surface antigens were observed in patients with non-specific inflammatory bowel diseases. In 6 of 12 patients the skin test results were negative. The clinical symptoms, which may suggest the maintaining inflammation of the intestinal mucosa were found in 50% of patients examined after surgical procedure because of Crohn's disease. The results suggest there are significant disturbances of cellular immunoreactivity parameters in patients with non-specific inflammatory bowel diseases. It may be one of the causes, which maintain the chronic inflammation of the intestinal mucosa and favour disease recurrence. The cellular immunoreactivity disturbances demonstrated in this study may be the result of the still persisting intestinal mucosa. Inflammation, in spite of deficiency of synonymous clinical symptoms indicating the disease recurrence.

Changes in the phagocytic cells in children treated with continuous ambulatory peritoneal dialysis.

Children on continuous ambulatory peritoneal dialysis (CAPD) in endstage renal failure are highly exposed to peritonitis. Peritoneal macrophages (PM) and blood neutrophils (PMNC) are the first line of defense against invading microbes. This study was undertaken for assessing surface receptors expression on PM and PMNC and to check their ability to phagocytosis and killing of bacteria. We have found that in spite of the decreased number of PM in dialysate fluid their viability and activity significantly increased during CAPD. Moreover, higher number of PM expressed CD16 and CD35 antigens (FcRIII and C3bR, respectively) in comparison with the results observed at CAPD onset. The number of PMNC expressed of these two antigens in uremic children blood were significantly lower in comparison with healthy control. The number of CD16 positive cells increased under influence of CAPD only temporarily. CAPD caused improvement of phagocytosis and intracellular killing of bacteria by PM but not by PMNC. There is discussed here influence of uremia and CAPD on surface antigens, function of phagocytes as well as renewal of PM during CAPD.

[Selected cellular immunity parameters in patients with Lesniowski-Crohn disease]. / Wybrane parametry odpornosci komórkowej u chorych z choroba Lesniowskiego--Crohna.

The study was aimed at investigating the expression of HLA-DR, CD4, CD8, CD56 and CD16 surface antigens on peripheral blood lymphocytes in patients with Crohn's disease. The percentage of rosette-forming T-cells in theophylline test was assessed simultaneously. The experiment at group consisted of 18 patients (aged 16-66) of long standing Crohn's disease history, being in the remission period and not treated by steroid antiinflammatory drugs. The percentage of lymphocytes with HLA-DR, CD4, CD8, CD56 and CD16 surface antigens was examined by means of the monoclonal antibodies (DAKO), and the APAAP procedure. The immunological test included also an assessment of the number of T-cells forming active (ARFC) and total (TRFC) rosettes in the theophylline test. Significantly increased (p < 0.001) percentage of lymphocytes with HLA-DR surface antigens was observed in patients with Crohn's disease. The percentage of lymphocytes with CD4, CD8, CD56 and CD16 surface antigens did not differ significantly from the control group, although the tendency for increase in the percentage of lymphocytes with CD8 surface antigens was clearly marked. The ratio of lymphocytes with CD4 to CD8 surface antigens and the number of T-cells forming active and total rosettes were significantly lower (p < 0.05) in patients with Crohn's disease. In 10 of 18 patients the number of T-cells forming active and total rosettes was lower than 500 cells in 1 mm3 peripheral blood. In addition particular notice should be given to the fact that no T-cells reaction to theophylline was obtained in patients' group. The results suggest significant cellular immunoreactivity disturbances which may be the result of the persisting intestinal mucosa's inflammation in patients with Crohn's disease being in the remission period. The studies of peripheral blood lymphocytes with HLA-DR surface antigens may have significance in Crohn's disease's clinical monitoring.