A combined retrograde tracer and GABA-immunocytochemical study of the projection from nucleus interpositus posterior to the posterior lobe C2 zone of the cat cerebellum.

The extent to which the cells of origin of the cerebellar nucleocortical pathway are immunopositive for gamma-aminobutyric acid (GABA) was investigated in four cats using retrograde labelling of nucleocortical neurons in combination with immunocytochemistry. Neurons were retrogradely labelled by injection of fluorescent (coumarin)-tagged latex microspheres into the c2 zone in the rostral part of the paramedian lobule. The zone was identified electrophysiologically by the characteristics of the climbing fibre responses evoked on the cerebellar surface by percutaneous stimulation applied to the left and right forepaws in pentobarbitone-anaesthetized animals. Sections of the cerebellum containing the retrogradely labelled neurons were processed for GABA immunocytochemistry using a fluorescent (rhodamine)-tagged immunoglobulin. When viewed with epifluorescence microscopy and appropriate filter blocks the retrogradely labelled nucleocortical neurons could be visualized in the same sections as the GABA-immunopositive neurons. Almost all of a total of 254 labelled nucleocortical neurons were located in nucleus interpositus posterior, where a total of 711 GABAergic neurons were also found. None of these cells contained coumarin-tagged beads and displayed immunoreactivity for GABA (i.e. none was double-labelled). When compared by area of their cell body, the nucleocortical and GABA-immunopositive neurons appeared to form two partially overlapping populations. The mean cell area of the nucleocortical neurons was 620 +/- 233 microns2 (SD), whereas the GABA-immunopositive neurons were much smaller, with a mean cell area of 220 +/- 115 microns2. The results suggest that GABA does not play a major role in the nucleocortical pathway to the c2 zone of the rostral paramedian lobule of the cat cerebellum.

A study of vasodilator responses evoked by antidromic stimulation of A delta afferent nerve fibers supplying normal and reinnervated rat skin.

Experiments have been carried out on anaesthetized rats to investigate some properties of the vasodilator responses evoked in skin by stimulation of small myelinated, A delta-fibers. Both normal and reinnervated skin in the saphenous nerve field were studied. In unoperated animals it was found that the magnitude and time course of the responses recorded from a particular patch of skin remain stable over a period of hours, but the responses varied in magnitude in different parts of the saphenous nerve territory. A delta-fiber evoked vasodilatation responses recorded from reinnervated skin were generally very similar to those seen in normal skin except that they tend to be smaller. These observations, taken together with previous findings, suggest that this ability of cutaneous A delta-fibers to influence the vasculature in the vicinity of their sensory endings has some physiological significance. Unfortunately, attempts to link these skin blood flow responses with activity of single, identified A delta-fiber units were not successful.

An atlas of glycine- and GABA-like immunoreactivity and colocalization in the cochlear nuclear complex of the guinea pig.

The distribution and colocalization of gamma-aminobutyric acid (GABA)- and glycine-like immunoreactivity in the cochlear nuclear complex of the guinea pig have been studied to produce a light microscopic atlas. The method used was based on post-embedding immunocytochemistry in pairs of 0.5-micron-thick plastic sections treated with polyclonal antibodies against conjugated GABA and glycine respectively. Immunoreactive cells, presumably short axon neurones, predominated in the dorsal cochlear nucleus, with mostly single-GABA-labelled cells in the superficial layer, double-labelled in the middle, and single-glycine-labelled in the deep layers. A few large single-glycine-labelled cells, interpreted as commissural neurons, occurred in the ventral nucleus. Scattered double-labelled cells, probably Golgi cells, were seen in the granule cell domain. Immunolabeled puncta of all three staining categories occurred in large numbers throughout the complex, apposed to somata and in the neuropil, showing a differential distribution onto different types of neuron. Three immunolabeled tracts were noted: the tuberculoventral tract, the commissural acoustic stria, and the trapezoidal descending fibres. Most of the fibres in these tracts were single-labelled for glycine, although in the last mentioned tract single-GABA- and double-labelled fibres were also found. Some of the immunolabeled cell types described here are proposed as the origins of the similarly labelled puncta and fibres on the basis of known intrinsic connections.

Transneuronal effects triggered by saphenous nerve injury on one side of a rat are restricted to neurones of the contralateral, homologous nerve.

Transection and regeneration of the saphenous nerve on one side of a rat reduces the ability of the contralateral saphenous nerve to evoke plasma extravasation following antidromic nerve stimulation. It is proposed that a transneuronal signal--that is a signal that passes from injured neurones on one side of the body to intact ones on the other via the spinal cord--is involved in triggering this response. The present experiments have shown that the influence of this transneuronal signal is restricted to neurones of homologous nerves on the two sides of an animal, i.e. it passes from saphenous nerve neurones on one side to saphenous nerve neurones on the other; its influence does not seem to extend to neurones in other nerves, for example ones in either the ipsi- or contralateral sural nerves.

Anatomy of the cochlear nuclear complex of guinea pig.

The cyto- and fibre-architecture of the cochlear nuclear complex of the guinea-pig has been studied in serial sections using Nissl, Golgi and combined cell-myelin staining of normal material, and a silver degeneration method after cochlear ablation. The nuclear subdivisions and major cell types can be recognised on the basis of those found in the cat, but there are some differences between the two species in the precise distribution and morphology of the neurons. The rostrodorsal part of the anteroventral cochlear nucleus (AVCN) contains predominantly spherical bushy cells, but these cannot be readily divided into large and small types as in the cat. Globular bushy cells are seen in the caudal region of the AVCN, but the majority occur in the posteroventral cochlear nucleus (PVCN), in an area extending from the nerve root right up to the boundary of the dorsal cochlear nucleus (DCN). The octopus cells constitute a distinct region in the most dorsomedial part of the PVCN underneath the DCN. Giant cells are seen scattered around the nerve root region. Multipolar and small cells are seen throughout the non-granular regions of the ventral cochlear nucleus (VCN) except for the octopus cell area, but occur mainly in the more rostral regions of the PVCN. Small cells occur in greatest abundance in the thin cap area at the dorsal edge of the VCN below a superficial granule cell layer. The latter covers the dorsolateral surface of the VCN, and a lamina of granule cells partially separates the PVCN from the DCN. The DCN can be divided into four layers. The outermost molecular layer (layer 1) is separated from the deeper regions by a prominent layer of granule cells (layer 2) which also contains the pyramidal cells. Molecular layer stellate cells are seen in layer 1 and a staggered row of cartwheel neurons is found at the boundary between layers 1 and 2. Layer 3 contains the basal dendrites of the pyramidal cells and some small (vertical) cells, and is innervated by the descending branches of the cochlear nerve. The deepest layer 4, which contains multipolar cells and giant cells, does not appear to receive this direct cochlear input.