RESUMO
Purified lipopolysaccharide (LPS), isolated from BCG and purified protein derivative of tuberculin (PPD) were used as antigens in an enzyme-linked immunosorbent assay (ELISA) of sera from patients with malignant melanoma undergoing immunotherapy with BCG. It was found that LPS and ELISA are useful tools in the monitoring of humoral immune response against BCG.
Assuntos
Imunoterapia , Lipopolissacarídeos/uso terapêutico , Melanoma/terapia , Tuberculina/administração & dosagem , Formação de Anticorpos , Ensaio de Imunoadsorção Enzimática , Testes de Hemaglutinação , Humanos , Lipopolissacarídeos/análise , Melanoma/imunologiaRESUMO
The hemagglutination antigen isolated from Mycobacterium tuberculosis strain Aoyama B was characterized by immunoelectrophoresis using the International Reference System for Mycobacterial Antigens. It showed two precipitin bands, the major one being identical with antigen 1 (arabinomannan). The minor precipitin band formed a spur with antigen 2 (arabinogalactan), attesting to the presence of common determinant groups. The immunoelectrophoretic analysis of the hemagglutination antigen against concanavalin A also revealed 2 precipitin bands, the major one being identical with the single precipitin band formed between the reference antigen and concanavalin A. The 2 arabinomannans present seemed to differ in molecular weight.
