RESUMO
The activity of interferon-induced 2',5'-oligoadenylatesynthetase (2',5'-OA-synthetase) in thymocytes of rats simultaneously treated with ethanol and acetic zinc preparation was studied. The 2',5'-OA-synthetase activity was decreased by 75% after daily ethanol administration for 14 days. The changes were less pronounced at the late terms of the experiment. In vitro stimulation of enzyme activity by inducer cycloferon was more pronounced at the late terms of ethanol action. Combined administration of ethanol and acetic zinc increased the 2',5'-OA-synthetase activity (by 81% at 21th day and by 30% at 28th day) in comparison to the group of animals that consumed ethanol only. The zinc preparation augmented the stimulation of enzyme activity induced by cycloferon, the effect was most prominent at the late terms of the ethanol action (at 28th day). Probably, ethanol depresses the synthesis of interferon or disturbs the functioning of 2',5'-oligoadenylate cascade. The acetic zinc amplifies the interferon synthesis.
Assuntos
2',5'-Oligoadenilato Sintetase/metabolismo , Etanol/farmacologia , Timócitos/efeitos dos fármacos , Timo/efeitos dos fármacos , Acetato de Zinco/farmacologia , 2',5'-Oligoadenilato Sintetase/antagonistas & inibidores , Acridinas/farmacologia , Administração Oral , Consumo de Bebidas Alcoólicas/imunologia , Consumo de Bebidas Alcoólicas/metabolismo , Consumo de Bebidas Alcoólicas/patologia , Animais , Animais não Endogâmicos , Indutores de Interferon/farmacologia , Masculino , Cultura Primária de Células , Ratos , Timócitos/enzimologia , Timócitos/imunologia , Timócitos/patologia , Timo/enzimologia , Timo/imunologia , Timo/patologiaRESUMO
The interferon production by rat spleen and thymus lymphocytes in conditions of gastric juice hypoacidity, evoked by 28 days of omeprazole treatment, was studied. It was shown that the spontaneous and induced by PHA and cycloferone interferon titer increases in thymocyte and sleenocyte cell cultures under the action of omeprazole. This effect was more expressed in thymocytes. It might be a result of stimulation of interferon secretion by lymphoid cells. Probably the interferon synthesis intensifies in conditions of hypoacidity. This effect may be considered as the reaction of immune system on inflammation in gastro-intestinal tract.
Assuntos
Interferons/biossíntese , Baço/efeitos dos fármacos , Timócitos/efeitos dos fármacos , Timo/efeitos dos fármacos , Acridinas/farmacologia , Animais , Células Cultivadas , Inibidores Enzimáticos/efeitos adversos , Suco Gástrico/química , Suco Gástrico/efeitos dos fármacos , Concentração de Íons de Hidrogênio , Interferons/metabolismo , Masculino , Omeprazol/efeitos adversos , Fito-Hemaglutininas/farmacologia , Ratos , Baço/metabolismo , Baço/patologia , Estômago/efeitos dos fármacos , Estômago/fisiopatologia , Timócitos/metabolismo , Timócitos/patologia , Timo/metabolismo , Timo/patologiaRESUMO
It was shown that the activity of interferon-induced enzyme 2',5'-oligoadenylate-synthetase is suppressed in rat spleen lymphocytes under the chronic alcohol intoxication. The values of enzyme activity were minimal under the long-term action of etanol (21 day). The combined administration of zinc acetate and etanol to rats causes the increase of enzyme activity, the effect is most expressed on the late stages of alcohol intoxication development.
