Analyzing text recognition from tactually evoked EEG.

Tactual exploration of objects produce specific patterns in the human brain and hence objects can be recognized by analyzing brain signals during tactile exploration. The present work aims at analyzing EEG signals online for recognition of embossed texts by tactual exploration. EEG signals are acquired from the parietal region over the somatosensory cortex of blindfolded healthy subjects while they tactually explored embossed texts, including symbols, numbers, and alphabets. Classifiers based on the principle of supervised learning are trained on the extracted EEG feature space, comprising three features, namely, adaptive autoregressive parameters, Hurst exponents, and power spectral density, to recognize the respective texts. The pre-trained classifiers are used to classify the EEG data to identify the texts online and the recognized text is displayed on the computer screen for communication. Online classifications of two, four, and six classes of embossed texts are achieved with overall average recognition rates of 76.62, 72.31, and 67.62% respectively and the computational time is less than 2 s in each case. The maximum information transfer rate and utility of the system performance over all experiments are 0.7187 and 2.0529 bits/s respectively. This work presents a study that shows the possibility to classify 3D letters using tactually evoked EEG. In future, it will help the BCI community to design stimuli for better tactile augmentation n also opens new directions of research to facilitate 3D letters for visually impaired persons. Further, 3D maps can be generated for aiding tactual BCI in teleoperation.

Pirfenidone inhibits post-traumatic proliferative vitreoretinopathy.

PurposeThe purpose of the study was to evaluate the efficacy and safety of intravitreal pirfenidone for inhibition of proliferative vitreoretinopathy (PVR) in a model of penetrating ocular injury.Patients and methodsPenetrating trauma was induced on the retina of rabbit and treated either with 0.1 ml of phosphate-buffered saline (PBS) or 0.1 ml of 0.5% pirfenidone, and development of PVR was evaluated clinically and graded after 1 month. Histopathology and immunohistochemistry with transforming growth factor beta (TGFß), alpha smooth muscle actin (αSMA), and collagen-1 were performed to assess the fibrotic changes. Expression of cytokines in the vitro-retinal tissues at different time points following pirfenidone and PBS injection was examined by RT-PCR. Availability of pirfenidone in the vitreous of rabbit at various time points was determined by high-performance liquid chromatography following injection of 0.1 ml of 0.5% pirfenidone. In normal rabbit eye, 0.1 ml of 0.5% pirfenidone was injected to evaluate any toxic effect.ResultsClinical assessment and grading revealed prevention of PVR formation in pirfenidone-treated animals, gross histology, and histopathology confirmed the observation. Immunohistochemistry showed prevention in the expression of collagen-I, αSMA, and TGFß in the pirfenidone-treated eyes compared to the PBS-treated eyes. Pirfenidone inhibited increased gene expression of cytokines observed in control eyes. Pirfenidone could be detected up to 48 h in the vitreous of rabbit eye following single intravitreal injection. Pirfenidone did not show any adverse effect following intravitreal injection; eyes were devoid of any abnormal clinical sign, intraocular pressure, and electroretinography did not show any significant change and histology of retina remained unchanged.ConclusionThis animal study shows that pirfenidone might be a potential therapy for PVR. Further clinical study will be useful to evaluate the clinical application of pirfenidone.

Silk fibroin hydrogel as physical barrier for prevention of post hernia adhesion.

BACKGROUND: Adhesion formation remains a major complication following hernia repair surgery. Physical barriers though effective for adhesion prevention in clinical settings are associated with major disadvantages, therefore, needs further investigation. This study evaluates silk fibroin hydrogel as a physical barrier on polypropylene mesh for the prevention of adhesion following ventral hernia repair. STUDY DESIGN: Peritoneal explants were cultured on silk fibroin scaffold to evaluate its support for mesothelial cell growth. Full thickness uniform sized defects were created on the ventral abdominal wall of rabbits, and the defects were covered either with silk hydrogel coated polypropylene mesh or with plain polypropylene mesh as a control. The animals were killed after 1 month, and the adhesion formation was graded; healing response of peritoneum was evaluated by immunohistochemistry with calretinin, collagen staining of peritoneal sections, and expression of PCNA, collagen-I, TNFα, IL6 by real time PCR; and its adverse effect if any was determined. RESULTS: Silk fibroin scaffold showed excellent support for peritoneal cell growth in vitro and the cells expressed calretinin. A remarkable prevention of adhesion formation was observed in the animals implanted with silk hydrogel coated mesh compared to the control group; in these animals peritoneal healing was complete and predominantly by mesothelial cells with minimum fibrotic changes. Expression of inflammatory cytokines decreased compared to control animals, histology of abdominal organs, haematological and blood biochemical parameters remained normal. CONCLUSION: Therefore, silk hydrogel coating of polypropylene mesh can improve peritoneal healing, minimize adhesion formation, is safe and can augment the outcome of hernia surgery.

A novel segmentation approach for noisy medical images using intuitionistic fuzzy divergence with neighbourhood-based membership function.

Medical image segmentation demands higher segmentation accuracy especially when the images are affected by noise. This paper proposes a novel technique to segment medical images efficiently using an intuitionistic fuzzy divergence-based thresholding. A neighbourhood-based membership function is defined here. The intuitionistic fuzzy divergence-based image thresholding technique using the neighbourhood-based membership functions yield lesser degradation of segmentation performance in noisy environment. Its ability in handling noisy images has been validated. The algorithm is independent of any parameter selection. Moreover, it provides robustness to both additive and multiplicative noise. The proposed scheme has been applied on three types of medical image datasets in order to establish its novelty and generality. The performance of the proposed algorithm has been compared with other standard algorithms viz. Otsu's method, fuzzy C-means clustering, and fuzzy divergence-based thresholding with respect to (1) noise-free images and (2) ground truth images labelled by experts/clinicians. Experiments show that the proposed methodology is effective, more accurate and efficient for segmenting noisy images.

Molecular characterization of Echinococcus granulosus of Indian animal isolates on the basis of nuclear and mitochondrial genotype.

Sixty-six isolates of larval stage of Echinococcus granulosus, a known pathogenic parasite of man and animals were collected from cattle, buffalo, sheep, and goats. Single-stranded conformation polymorphism (SSCP) for analysis of variation after denaturation of amplicon of intron of actin II (ACTII) revealed six SSCP phenotypes. Intron portion was analyzed considering introns-early and introns-late theories. Isolates belonging to different conformers were further screened for mitochondrial ATPase subunit 6 (ATP6) and NADH dehydrogenase subunit II (nadII) genotypes. Assignment of each isolate to its specific strain was achieved after comparing with standard genotypes of E. granulosus. Variants deduced by nuclear targets did not match with mitochondrial haplotypes. A possible explanation for this observation can be attributed toward interspecific hybridization since cross-fertilization occurs less frequently in hermaphrodite organisms. A phylogenetic tree drawn on the basis of predicted aminoacid sequence of ATP6 and nadII revealed two distinct clusters i.e. E. granulosus sensu stricto and E. ortleppi/cattle strain (EG5). To the best of our knowledge, this is the first report of genetic characterization of two distinct ATP6 and nadII genotypes of zoonotic importance living in sympatry.

Mutation scan screening of Echinococcus granulosus isolates of Indian origin.

During the present investigation a total of forty Indian animal isolates were screened by single strand conformation polymorphism (SSCP) collected from sheep, goat, cattle and buffalo. The result of the study indicated that nuclear variants of Echinococcus granulosus were present in both small and large ruminants. SSCP phenotypes of AgB, intron of actin II and Hbx-2 have been deduced. Presence of nuclear variants due to mutation of E. granulosus has been discussed depending on hypotheses imparted earlier in literature. High polymophism of AgB demands further investigation because the gene is related with immune evasion and infectivity. This communication reports for the first time the comparative profile of Indian goat, sheep, cattle and buffalo isolates of E. granulosus complex.

Sarcoma-like mural nodules in ovarian mucinous cystadenomas--a report of two cases.

Sarcoma-like mural nodule is a very rare occurrence in a mucinous tumour of the ovary. Two such nodules having morphologically benign features with osteoclastic giant cells, in the wall of mucinous cystadenoma are described. In addition, these nodules exhibited reactive vascular proliferations. The sarcoma-like nodules are associated with a favourable outcome and must be distinguished from other malignant nodules composed of sarcoma or anaplastic carcinoma that may also occur in the wall of mucinous ovarian tumours.

Analysis of differentially expressed genes in hyperthyroid-induced hypertrophied heart by cDNA microarray.

Experiments were carried out to identify the altered genes in hyperthyroid rat heart and their influence on the functions of cardiac myocytes. Chronic treatment of rats with 3,5,3' triiodo-L-thyronine (T3) resulted in a prominent increase in the size of the left ventricle with increased wall thickness and reduced chamber volume leading to concentric cardiac hypertrophy. The heart weight to body weight ratio (HW/BW) in hyperthyroid rats was increased by about 58% over that of normal rats. Using cDNA microarray comprising 588 genes, we compared the differences in mRNA expression of hyperthyroid and normal rat heart. Based on a threshold of greater than 10% change, about 37 genes were found to be regulated by T3. Further analyses by Western blotting, Northern blotting and real-time quantitative RT-PCR of some of the genes confirmed the microarray results. The T3-altered genes encode various types of proteins related to metabolism, matrix and cytoskeletal structures, growth factors, transcription factors, Ca(2+)-channels etc. The physiological significance of one of these altered proteins in hyperthyroid heart, insulin-responsive glucose transporter (GLUT) type 4 (GLUT4), was studied in detail. The expression of GLUT4 was drastically reduced in the ventricular tissues of hyperthyroid heart. Insulin-induced glucose uptake in hyperthyroid cardiomyocytes was reduced significantly, indicating the impaired glucose transport in cardiac cells. Interestingly, a few genes such as GLUT4, cytochrome P450 isoforms, superoxide dismutase (SOD), collagens, matrix metalloproteinases (MMP), tissue inhibitors of matrix metalloproteinases etc. which had not been reported earlier were found to be altered in hyperthyroid heart. Our results show some new aspects of hyperthyroid heart which will be important in assessing the pathophysiology of hypertrophied cardiomyocytes.

Transcatheter therapy of intravisceral bleeding.

Intravisceral bleeding is a life-threatening situation demanding fast and active steps to control, unless it stops spontaneously as in a few lucky patients. A surgical approach is a major intervention with its associated procedural risks when the site is in organs other than the spleen. The transcatheter approach helps in precise location and embolization of the bleeding site; it is a more practical approach that has been known for the past 2 decades to be effective and provide good long-term results. However, the transcatheter approach has been reported in fewer than 200 cases in the literature. Our small series of 43 cases shows initial success of 95% and a fatal early recurrence of only 2.4%. Non-hemorrhagic etiology (sepsis, head injury, etc.) was the major cause of early death (14.6%) among the successfully embolized cases. Long-term follow-up shows recurrence (4.8%) only in the group of chronic etiology. Transcatheter embolization of intravisceral bleeding with Gel-Foam and/or poly-vinyl alcohol particles is a swift, effective and precise method of treatment without major operational hazards.

Response to low-dose interferon in chronic liver disease due to hepatitis B virus infection.

BACKGROUND: Interferon is at present the only effective therapy for chronic hepatitis B virus (HBV) infection. Data regarding its efficacy in India are scant. The present study was undertaken to assess the efficacy of low-dose interferon in chronic liver disease due to HBV infection. METHODS: Twenty four patients with histologic evidence of chronic hepatitis with or without cirrhosis, and persistent elevation of serum aminotransferases and persistent positivity for HBsAg and HBeAg for more than six months, were included. Fourteen patients were treated with interferon alpha-2b, 3 million units thrice weekly for 16 weeks; ten patients who could not afford the drug were followed up as controls without specific therapy. Patients were examined weekly for the first 4 weeks, followed by two weekly for 12 weeks and then every two months. Blood tests for viral markers and liver biochemistry were done at 0, 4, 8, 12, 16 weeks and then at two-month intervals for at least one year after therapy. Patients who cleared HBeAg were followed up for 2.2 (1-4) years for HBsAg clearance. RESULTS: HBeAg clearance occurred in 9 patients (64%) in the interferon group, and in one control patient (p < 0.01). HBsAg clearance occurred in only one patient in the treatment group during follow up of mean 2.4 years. No patient in the control group cleared HBsAg. Patients having high ALT level at the beginning of treatment had significantly higher HBeAg clearance rate (7 of 7) than patients with low ALT levels (2 of 7; p < 0.05). CONCLUSION: Low-dose interferon therapy is effective in attaining HBeAg, but not HBsAg, clearance in chronic HBV infection.

Natural history of severe duodenal ulcer disease.

BACKGROUND: A subset of patients with chronic duodenal ulcer has severe ulcer diathesis in the form of frequent relapses and complications like perforation and hemorrhage. We observed the effect of drug treatment on the natural history of this subset. METHODS: Of 526 patients diagnosed to have chronic duodenal ulcer by endoscopy, 23 patients with severe diathesis were available for long follow-up (mean period 36 months). Each patient was assessed clinically and endoscopically every 2 months for at least 12 months and then every 3 months or when symptomatic. Helicobacter pylori status was assessed during endoscopy. The effect of antisecretory drugs and anti-H. pylori therapy on natural history was determined. RESULTS: Thirteen of 23 patients (56%) had refractory ulcers; six responded to double dose of H2-receptor antagonists (H2RA) for 8 weeks and six to omeprazole 40 mg daily for 4-8 weeks. Of 20 patients (87%) who were H. pylori-positive, 15 completed triple-drug therapy; of these, 10 patients eradicated H. pylori. These 10 patients were followed up for 24 months; there were no ulcer relapses within the first 12 months but 8 of them relapsed between 12 and 24 months (total number of relapses 8). Reinfection with H. pylori occurred in 3 patients. In the other 10 patients who remained H. pylori-positive, there were 19 episodes of ulcer relapse in 7 patients over 24 months, in spite of maintenance therapy with H2RA (p < 0.05). CONCLUSIONS: Refractoriness in patients with severe ulcer disease is usually episodic and amenable to larger doses of omeprazole or H2RA. Anti-H. pylori therapy improves the natural history but its effect in preventing ulcer relapse is short lasting (less than 12 months). Recurrence of infection is a problem in our population.

Cognitive reasoning using fuzzy neural nets.

The paper presents a new model for cognitive reasoning using fuzzy neural nets. The analysis of the proposed model yields guaranteed stability of the temporal fuzzy inferences, derived from the network and conditional stability of the structure of the cognitive map, framed by the arcs of the network. The results arrived at in the paper have been illustrated with reference to a typical weather forecast system.

Collision tumor of gastrointestinal tract.

A rare combination of multiple adenomatous polyps of the colon and carcinoid tumor of the ileum in a 54-year-old man is reported.

Fate of [14C]coumaphos after dermal application to lactating goats as a pour-on formulation.

Two lactating Nubian goats were dermally treated with [14C]coumaphos (O-[3-chloro-4-methyl-2-oxo-2H-benzopyran-7-yl] O,O-diethyl phosphorothioate) as a 4% active ingredient pour-on formulation. Doses were administered, along the dorsal midline from withers to sacrum, at a rate equivalent to 14 mg of coumaphos/kg of body weight. During the 7 days after treatment, an average of less than 0.1, 4.7, and 1% of the administered dose was eliminated in the milk, urine, and feces, respectively. When goats were killed after 7 days, about 45% of the administered radiocarbon remained on the hair and skin, and this consisted almost entirely of intact coumaphos. Residues in selected tissues collected after 7 days were, in every case, less than 1 mg of coumaphos equivalent/kg of tissue, with highest residues in adipose tissue, followed by residues in kidney and liver. In milk, residue amounts plateaued after about 2 days and remained relatively constant at about 0.1 mg of coumaphos equivalent/kg of milk. Residues in adipose tissue and in milk consisted mainly of unmetabolized coumaphos. In urine, most radiocarbon was present as metabolites of coumaphos, but in feces, most radiocarbon was present as the intact parent compound. Coumaphos was absorbed slowly and at a constant rate after dermal application to lactating goats as a pour-on formulation.

Protein losing enteropathy as a sole manifestation of non-Hodgkin's lymphoma.

We describe a patient who presented with oedema and hypoproteinaemia which was proved to be due to protein-losing enteropathy. Extensive gastrointestinal investigations failed to discover any definitive cause. He subsequently developed tuberculous inguinal lymphadenopathy but although treated for tuberculosis the protein-losing state persisted. Later on he developed renal failure due to obstructive nephropathy. Laparotomy was carried out and it disclosed the diagnosis of non-Hodgkin's lymphoma, treatment of which cured the protein-losing state.