[Characteristics of the serologic antibacterial response of patients with diphtheria and of bacterial carriers]. / Osobennosti serologicheskogo antibakterial'nogo otveta u bol'nykh difteriei i bakterionositelei.

The enzyme immunoassay system for the detection of specific antibacterial antibodies to Corynebacterium diphtheriae membrane protein (mol. wt. 64 KD) has been used. The physicochemical nature of these antibacterial antibodies has been established and their quantitative characteristics have been determined. The analysis of the dynamics of the formation of antimicrobial IgG- and IgM-antibodies in diphtheria patients (on days 3-28 of the disease) has shown that by the time of convalescence the antibody level increases about 200-fold (p less than 0.0001). The possibility of using the dynamics of the formation of specific antimicrobial IgM-antibodies for the serodiagnosis of carrier state is shown, even if the increase of the level of antimicrobial IgG-antibodies is insufficient for this purpose (not more than twofold).

[Immunomodulating properties of the antigens of the cell walls of nontoxigenic Corynebacterium diphtheriae]. / Immunomoduliruiushchie svoistva antigenov kletochnykh stenok netoksigennykh korinebakterii difterii.

The total protein fraction was obtained by extraction with nonionic detergent NP-40. The fraction composition and molecular parameters were studied with DDS-Na electrophoresis in polyacryl amide gel. Common properties of the Corynebacterium diphtheriae protein fraction 66 kD and an analogous fraction of the cell walls of staphylococci and the measles virus were shown with immunoblotting. Cell-mediated mechanisms of the immune response to contact with antigens of Corynebacterium diphtheriae cell walls were revealed.

[Gamma-cystathionase: the non-ideal gel filtration high performance liquid chromatography. Physico-chemical and immunochemical characteristics of the enzyme]. / Gamma-tsistationaza: vysokoéffektivnaia zhidkostnaia khromatografiia v rezhime neideal'noi gel'-fil'tratsii. Fiziko-khimicheskaia i immunokhimicheskaia kharakteristika fermenta.

The rapid method for gamma-cystathionase purification was developed. It is based on the non-ideal gel filtration HPLC. The isolated homogeneous enzyme was used for immunization and immunosorbent preparation. A monospecific polyclonal antibody was prepared. The substrate specificity of the isolated enzyme was studied.

[Use of immunoenzyme analysis for determining antibacterial antibodies in diphtheria infection]. / Ispol'zovanie immunofermentnogo analiza dlia opredeleniia antibakterial'nykh antitel pri difteriinoi infektsii.

A diagnostic EIA system for the detection of antibacterial antibodies in diphtheria infection has been developed. As antigen, homogeneous membrane protein (mol. wt. 64 KD) obtained from Corynebacterium diphtheriae cell walls has been used. This protein antigen has been prepared with the use of nonionic detergent NP-40.

[Isolation and immunochemical characteristics of soluble membrane proteins from the cell wall of Corynebacterium diphtheria]. / Vydelenie i immunokhimicheskaia kharakteristika rastvorimykh membrannykh belkov kletochnykh stenok korinebakterii difterii.

A method for isolation of immunochemically active proteins from Corynebacterium diphtheria membranes was elaborated. The proteins were solubilized with the nonionic detergent NP-40 and gel-filtered through an Ultrogel AcA-34 column under denaturating conditions. The purified proteins (Mr = 64 kD) were antigenically active in a solid phase radioimmunoassay with human antidiphtheria antibodies.