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Biokhimiia ; 58(10): 1616-24, 1993 Oct.
Artigo em Russo | MEDLINE | ID: mdl-8268304

RESUMO

The heterogeneity and some properties of DNA-methylases isolated from nonmalignant human thyroid formations--nodular and diffuse goiters--have been studied. Isoelectrofocusing of methylase preparations produced 6-7 distinct activity peaks distinguished by pI, activity towards Ca2+ and Mg2+, sensitivity towards dithiothreitol and capacity to methylase cytosine into mono-, di- and tripyrimidine blocks in vitro. The degree of DNA methylation in vivo depended on the origin of of the goiter. Analysis of several methylase fractions by two-dimensional electrophoresis performed according to O'Farrell revealed the presence of major polypeptides having similar molecular masses (approximately 36 kDa). Besides the major component, all the preparations under study contained 6-10 characteristic minor components. The data obtained are suggestive of a structural and functional heterogeneity of human methylases.


Assuntos
Metilases de Modificação do DNA/metabolismo , Bócio Nodular/enzimologia , Doença de Graves/enzimologia , Glândula Tireoide/enzimologia , Eletroforese em Gel Bidimensional , Humanos , Focalização Isoelétrica , Peso Molecular
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