[Research on the association between Borna disease virus infection and the viral encephalitis].

OBJECTIVE: To investigate the infection of Borna disease virus (BDV) in unidentified viral encephalitis patients in Ningxia in order to explore if the nucleotide sequence and amino acid sequence in BDV p24 were homophylic with the overseas standard strain. We also intended to investigate the correlation between BDV infection and the unidentified viral encephalitis patients from Ningxia to lay an experimental basis for etiological diagnosis, prevention and treatment on certain human neuropsychiatric disorders. METHODS: BDV p24 gene fragment was detected by nested reverse transcriptase polymerase chain reaction with fluorescence quantitative PCR (FQ-nRT-PCR) in peripheral blood mononuclear cells (PBMCs). Samples were from 59 unidentified viral encephalitis patients and 60 normal controls. For those positive products, gene sequence and amino acid sequence were then analyzed by BLAST and DNAsist 5.0. RESULTS: The positive rate of the BDV p24 gene fragment in PBMCs from the unidentified viral encephalitis (10.17%) was significantly higher than from the controls (0%) (P < 0.05). Data from the gene sequence on those positive products showed BDV p24 fragment in the patients with unidentified viral encephalitis from Ningxia was homophylic with strain H3915 detected from ill horses (97.67%), as well as with the strain H1766 (96.51%) and strain V (95.35%). However, their amino acid sequences remained the same. CONCLUSION: BDV infection might probably have existed in the unidentified viral encephalitis patients of Ningxia. The gene sequence seemed to be homophylic with that of standard strain H1766 and strain V, especially with strain H3915.

N'-(3-Ethoxy-2-hydroxybenzylidene)-benzenesulfonohydrazide.

There are two mol-ecules in the asymmetric unit of the title compound, C(15)H(16)N(2)O(4)S, both of which are stabilized by an intra-molecular O-H⋯N hydrogen bond. Inter-molecular N-H⋯O hydrogen bonds lead to [101] chains of mol-ecules in the crystal structure.

1-Hydroxymethyl-3,12-dioxa-14-aza-tetracyclo[9.2.1.0.0]tetradecane.

In the title fused-ring compound, C(12)H(13)NO(3), the two five-membered C(3)NO rings both approximate to envelope conformations with C atoms in the flap positions. The OH group of the pendant CH(2)OH unit is disordered over two positions in a 0.528 (5):0.472 (5) ratio. One of the OH groups participates in an O-H⋯N hydrogen bond, generating centrosymmetric dimers in the crystal structure.

Hexaaqua-cobalt(II) bis-[4-(2-hydroxy-benzyl-ideneamino)-benzene-sulfonate].

In the cation of the title compound, [Co(H(2)O)(6)](C(13)H(10)NO(4)S)(2), the Co atom lies on a centre of symmetry and its coordination geometry is octahedral. The crystal structure is stabilized by water-anion O-H⋯O hydrogen bonds. An intra-molecular O-H⋯N hydrogen bond occurs in the anion.

2-Acetyl-3-methyl-pyrazine phenyl-sulfonyl-hydrazone.

In the title compound, C(13)H(14)N(4)O(2)S, the dihedral angle between the aromatic rings is 55.42 (14)°. In the crystal structure, an N-H⋯O hydrogen bond leads to chains of mol-ecules along [001].

4-(Diethyl-amino)salicylaldehyde phenyl-sulfonyl-hydrazone.

In the title compound, C(17)H(21)N(3)O(3)S, the dihedral angle between the aromatic ring planes is 84.2 (2)°. The pendant ethyl groups of the -N(C(2)H(5))(2) group are disordered over two sets of positions in a 0.84 (2):0.16 (2) ratio. The mol-ecular conformation is stabilized by an intra-molecular O-H⋯N hydrogen bond, and inter-molecular N-H⋯O bonds lead to [010] chains in the crystal structure.