RESUMO
Objective: To explore the repair effect of tissue engineering for annulus fibrosus (AF) injury in stress-stimulation environment. Methods: Non-adhesive fibrinogen (Fib) representing the repair with non-stress stimulation and adhesive hydrogel of fibrinogen, thrombin and genipin mixture (Fib-T-G) representing the repair with stress stimulation were prepared to repair the AF lesion. The relationship between adhesion and stress stimulation was studied in rheological measurements, tension tests and atomic force microscopy (AFM) experiments. The repair effect of stress stimulation was studied in designed acellular AF scaffold models with fissures and defects. The models were repaired by the two different hydrogels, then implanted subcutaneously and cultured for 21 âd in rats. Histology and qPCR of COL1A1, COL2A1, aggrecan, RhoA, and ROCK of the tissue engineering of the interface were evaluated afterward. Moreover, the repair effect was also studied in an AF fissure model in caudal disc of rats by the two different hydrogels. Discs were harvested after 21 âd, and the disc degeneration score and AF healing quality were evaluated by histology. Result: In interfacial stress experiment, Fib-T-G hydrogel showed greater viscosity than Fib hydrogel (24.67 â± â1.007 vs 459333 â± â169205 âmPa âs). Representative force-displacement and sample modulus for each group demonstrate that Fib-T-G group significantly increased the interfacial stress level and enhanced the modulus of samples, compared with Fib group (P â< â0.01). The Fib-T-G group could better bond the interface to resist the loading strain force with the broken point at 1.11 â± â0.10 âN compared to the Fib group at 0.12 â± â0.08 âN â(P â< â0.01). Focusing on the interfacial healing in acellular AF scaffold model, compared with Fib â+ âMSCs group, the fissure and defect were connected closely in Fib-T-G â+ âMSCs group (P â< â0.01). Relative higher gene expression of COL2A1 and RhoA in Fib-T-G â+ âMSCs group than Fib â+ âMSCs group in AF fissure and AF defect model (P â< â0.05). The immunohistochemistry staining showed more positive staining of COL2A1 and RhoA in Fib-T-G â+ âMSCs group than in Fib â+ âMSCs group in both AF fissure and AF defect models. The degree of disc degeneration was more severe in Fib â+ âMSCs group than Fib-T-G â+ âMSCs group in vivo experiment (11.80 â± â1.11 vs 7.00 â± â1.76, P â< â0.01). The dorsal AF defect in Fib-T-G â+ âMSCs group (0.02 â± â0.01 âmm2) was significantly smaller than that (0.13 â± â0.05 âmm2) in Fib â+ âMSCs group (P â< â0.05). Immunohistochemical staining showed more positive staining of COL2A1 and Aggrecan in Fib-T-G â+ âMSCs group than in Fib â+ âMSCs group. Conclusion: Genipin crosslinked hydrogel can bond the interface of AF lesions and transfer strain force. Stress stimulation maintained by adhesive hydrogel promotes AF healing. The translational potential of this article: We believe the effect of stress stimulation could be concluded through this study and provides more ideals in mechanical effects for further research, which is a key technique for repairing intervertebral disc in clinic. The adhesive hydrogel of Fib-T-G+MSCs has low toxicity and helps bond the interface of AF lesion and transfer strain force, having great potential in the repair of AF lesion.
RESUMO
OBJECTIVE: To evaluate the therapeutic effects of Guliu capsule (GLC) combined with 89Sr in treating metastatic bone tumors. METHODS: On the basis of random sampling and grouping, 50 patients with metastatic bone tumors were selected to receive combined treatment with GLC and (89)Sr with another 50 patients recruited for (89)Sr treatment alone. The therapeutic effect of the therapies were evaluated according to the relief of ostalgia and quality-of life (QOF), local bone metabolism and hematopoietic function of the bone marrow. RESULTS: The efficacy rate of GLC+89Sr and (89)Sr in relieving ostalgia was 94.0% and 76.0%, and the rate of QOF improvement and stabilization was 94.0% and 74.0%, respectively, both showing significant difference between the two groups (P<0.05). The effect of the two treatment protocols on local bone metabolism and their hemotoxicity were also significantly different (P<0.05, P<0.01, respectively). CONCLUSION: Combined treatment with GLC and (89)Sr is effective for metastatic bone tumor and improves the patient's QOF with enhanced ostalgia relief rate and decreased hemotoxicity.
Assuntos
Neoplasias Ósseas/radioterapia , Neoplasias Ósseas/secundário , Medicamentos de Ervas Chinesas/uso terapêutico , Radioisótopos de Estrôncio/uso terapêutico , Adolescente , Adulto , Idoso , Cápsulas , Terapia Combinada , Feminino , Humanos , Masculino , Pessoa de Meia-IdadeRESUMO
OBJECTIVE: To study the effect of chemotherapy assisted with shenqi fuzheng injection (SFI) on senile patients with non-small cell lung cancer (NSCLC). METHODS: One hundred and twenty old patients with NSCLC were treated with NP chemotherapeutic protocol, to the 60 patients in the treated group among them, additional medication of SFI was started one week before the beginning of chemotherapy. The short-term therapeutic efficacy, long-term survival rate, changes on quality of life (QOL) and immune function of patients, and hematological toxicity of therapy were observed. RESULTS: Difference between the two groups on short-term therapeutic effect, 1- and 2-year survival rate showed no significance (P>0.05), but the 3-year survival rate in the treated group was 26.6%, while that in the control group was 11.7%, showed that the former was higher than the latter. After treatment QOL of the treated group was better than that of the control (P<0.05). Besides, the hematological toxicity and affection on immune function of chemotherapy after treatment in the treated group were all lower than those in the control group showing significant difference (P<0.05). CONCLUSION: SFI has definite toxicity relieving effect on chemotherapy in treating senile NSCLC.
